Journal: Sensors (Basel, Switzerland)

Article Title: An Aptamer-Based Biosensor for Direct, Label-Free Detection of Melamine in Raw Milk

doi: 10.3390/s18103227

Figure Lengend Snippet: A schematic representation of the design of an aptamer-DNAzyme conjugated biosensor for microarray screening. The designed biosensor comprises an aptamer region (red), a DNAzyme region (blue), an internal loop region (cyan), two block sequences (gray) for the aptamer and the DNAzyme, and a linker region, immobilized on the microarray electrode.

Article Snippet: The 1200 unique sequences, 1180 for sensor screening, and 20 as control sequences, were randomly located on the 12K ElectraSense microarray chip purchased from Recenttec K.K. (Tokyo, Japan), with ten replicates.

Techniques: Microarray, Blocking Assay

Journal: Frontiers in Microbiology

Article Title: Transcriptional Changes Underlying Elemental Stoichiometry Shifts in a Marine Heterotrophic Bacterium

doi: 10.3389/fmicb.2012.00159

Figure Lengend Snippet: Schematic of microarray data analysis . Probe counts are shown in italicized font and gene counts are in bold font. (A) Significant Analysis of Microarray (SAM) was used to analyze the microarray data (pairwise SAM comparisons with 5,000 permutations and a false discovery rate ≤ 2%). From these results, probes significantly different in all three comparisons to the other treatments (that is, falling into the three-way overlap region of each Venn diagram) were selected for further analyses. (B) From the normalized fluorescence values, the fold-change for each probe was calculated as the median value between all limitation-to-excess pairwise comparisons and genes whose fold-change was ≥ 3 were retained.

Article Snippet: The CustomArray 12 K microarray slides for R. pomeroyi DSS-3 used in this study were described in detail by Bürgmann et al. ( ).

Techniques: Microarray, Fluorescence