Journal: PLoS ONE

Article Title: Inflammatory response of mesenchymal stromal cells after in vivo exposure with selected trauma-related factors and polytrauma serum

doi: 10.1371/journal.pone.0216862

Figure Lengend Snippet: (A) Analysis of cytokines in serum from healthy volunteers (control; n = 5), in AB-serum (n = 1) and in serum from polytrauma (PT) patients (n = 5). Mean and SD are shown. Cytokine levels in serum from PT patients were compared to cytokine levels in serum from healthy volunteers. Statistical significance was assessed via student's t-test (with correction for multiple comparisons), * P < 0.05. IL6: interleukin 6; IL10: interleukin 10; CXCL1: C-X-C motif chemokine ligand 1; CCL2: C-C motif chemokine ligand 2. (B) Effect of serum from PT patients on proliferation of MSC. Three MSC preparations and five individual sera from PT patients were used (randomized combination). Serum was collected from PT patients 0/4/12/24/48/120/240 h after admission to hospital. MSC cell count was determined by fluorescence intensity and compared to cell growth in Alpha-MEM without (negative control) or with supplement (positive control).

Article Snippet: The PTCH consisted of 10 ng/ml IL1B (PeproTech, Hamburg, Germany), 10 ng/ml IL6 (BIOMOL, Hamburg, Germany), 500 ng/ml C3a, 100 ng/ml C5a (both Calbiochem, Darmstadt, Germany), 60 ng/ml HMGB1, 300 ng/ml THBD, 450 pg/ml THPO.

Techniques: Control, Cell Counting, Fluorescence, Negative Control, Positive Control

Journal: PLoS ONE

Article Title: Inflammatory response of mesenchymal stromal cells after in vivo exposure with selected trauma-related factors and polytrauma serum

doi: 10.1371/journal.pone.0216862

Figure Lengend Snippet: Left column: Cells were cultured in medium supplemented with 20% AB-serum with or without (-, three biological replicates, 2 technical replicates) addition of trauma factors (interleukin 1 beta (IL1B, n = 3), polytrauma cocktail high (PTCH, n = 3), polytrauma cocktail low (PTCL, n = 3)) for 6 h or 24 h. Statistical significance was assessed via two-way ANOVA (with correction for multiple comparisons). Right column: cells were cultured in medium supplemented with 20% AB-serum (three biological replicates, 2 technical replicates) or serum from polytrauma patients (PTS) taken at certain times (0 h, 4 h, 12 h, 24 h, 48 h, 120 h, 240 h; three different MSC preparations and sera from five different patients were used for each point of time) for 6 h. Cytokine levels of cell culture supernatant from PTS-treated MSC were compared to cytokine levels of cell culture supernatant from AB-serum-treated MSC. Statistical significance was assessed via student's t-test (with correction for multiple comparisons), * P < 0.05. IL6: interleukin 6; IL10: interleukin 10; VEGFA: vascular endothelial growth factor-A; CXCL1: C-X-C motif chemokine ligand 1; CCL2: C-C motif chemokine ligand 2.

Article Snippet: The PTCH consisted of 10 ng/ml IL1B (PeproTech, Hamburg, Germany), 10 ng/ml IL6 (BIOMOL, Hamburg, Germany), 500 ng/ml C3a, 100 ng/ml C5a (both Calbiochem, Darmstadt, Germany), 60 ng/ml HMGB1, 300 ng/ml THBD, 450 pg/ml THPO.

Techniques: Cell Culture