10g11 Search Results


10g11  (Progen Biotechnik)
90
Progen Biotechnik 10g11
Cell–cell adhesion via E-cadherin and <t>desmoglein-2</t> occurs by a cooperative mechanism in both E-variants and TPA-treated R-variants. Monolayers of HCT-8/R1 cells ( R ) were treated with 250 ng/ml TPA as indicated in Materials and Methods. Monolayers of HCT-8/E8 cells ( E ) were not treated with TPA here ( b and d ), although analysis of TPA-treated E-variants yielded essentially identical data (not shown). Both R- and E-variants were analyzed for cell aggregation (increasing particle volume) either after 0 min (broken lines in a and b ; peak positions indicated by 0-min arrows in c and d ) or after 30 min of incubation at 37°C (full lines in all panels). Aggregation for 30 min by R-variants, which were not treated with TPA, yielded curves coinciding with the 0-min curves (not shown). Other curves depicted correspond with the following antibody treatments (see also Materials and Methods). The mAb MB-2 against human E-cadherin (O) was used at an effective concentration (dilution 1:20; a and b ), or at a more ineffective concentration (1:200; c and d , with in c only peak position indicated by an arrow). The mAb <t>10G11</t> against desmoglein-2 (□) was also used at an effective concentration (1:2; a and b ), or at an ineffective concentration (1:8; c and d , with in d only peak position indicated by an arrow). These two antibodies, both at the lower concentrations, were also combined as indicated by filled circles (•) in c and d. Upon such combination, a clear-cut synergistic inhibition of cell aggregation was observed for both cell types.
10g11, supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90/100 stars
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anti-plvap antibody 10g11.3.1  (Genentech inc)
90
Genentech inc anti-plvap antibody 10g11.3.1
Cell–cell adhesion via E-cadherin and <t>desmoglein-2</t> occurs by a cooperative mechanism in both E-variants and TPA-treated R-variants. Monolayers of HCT-8/R1 cells ( R ) were treated with 250 ng/ml TPA as indicated in Materials and Methods. Monolayers of HCT-8/E8 cells ( E ) were not treated with TPA here ( b and d ), although analysis of TPA-treated E-variants yielded essentially identical data (not shown). Both R- and E-variants were analyzed for cell aggregation (increasing particle volume) either after 0 min (broken lines in a and b ; peak positions indicated by 0-min arrows in c and d ) or after 30 min of incubation at 37°C (full lines in all panels). Aggregation for 30 min by R-variants, which were not treated with TPA, yielded curves coinciding with the 0-min curves (not shown). Other curves depicted correspond with the following antibody treatments (see also Materials and Methods). The mAb MB-2 against human E-cadherin (O) was used at an effective concentration (dilution 1:20; a and b ), or at a more ineffective concentration (1:200; c and d , with in c only peak position indicated by an arrow). The mAb <t>10G11</t> against desmoglein-2 (□) was also used at an effective concentration (1:2; a and b ), or at an ineffective concentration (1:8; c and d , with in d only peak position indicated by an arrow). These two antibodies, both at the lower concentrations, were also combined as indicated by filled circles (•) in c and d. Upon such combination, a clear-cut synergistic inhibition of cell aggregation was observed for both cell types.
Anti Plvap Antibody 10g11.3.1, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-plvap antibody 10g11.3.1/product/Genentech inc
Average 90 stars, based on 1 article reviews
anti-plvap antibody 10g11.3.1 - by Bioz Stars, 2026-02
90/100 stars
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10g11 (desmoglein 2) antibody  (Cymbus Biotechnology)
90
Cymbus Biotechnology 10g11 (desmoglein 2) antibody
Cell–cell adhesion via E-cadherin and <t>desmoglein-2</t> occurs by a cooperative mechanism in both E-variants and TPA-treated R-variants. Monolayers of HCT-8/R1 cells ( R ) were treated with 250 ng/ml TPA as indicated in Materials and Methods. Monolayers of HCT-8/E8 cells ( E ) were not treated with TPA here ( b and d ), although analysis of TPA-treated E-variants yielded essentially identical data (not shown). Both R- and E-variants were analyzed for cell aggregation (increasing particle volume) either after 0 min (broken lines in a and b ; peak positions indicated by 0-min arrows in c and d ) or after 30 min of incubation at 37°C (full lines in all panels). Aggregation for 30 min by R-variants, which were not treated with TPA, yielded curves coinciding with the 0-min curves (not shown). Other curves depicted correspond with the following antibody treatments (see also Materials and Methods). The mAb MB-2 against human E-cadherin (O) was used at an effective concentration (dilution 1:20; a and b ), or at a more ineffective concentration (1:200; c and d , with in c only peak position indicated by an arrow). The mAb <t>10G11</t> against desmoglein-2 (□) was also used at an effective concentration (1:2; a and b ), or at an ineffective concentration (1:8; c and d , with in d only peak position indicated by an arrow). These two antibodies, both at the lower concentrations, were also combined as indicated by filled circles (•) in c and d. Upon such combination, a clear-cut synergistic inhibition of cell aggregation was observed for both cell types.
10g11 (Desmoglein 2) Antibody, supplied by Cymbus Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/10g11 (desmoglein 2) antibody/product/Cymbus Biotechnology
Average 90 stars, based on 1 article reviews
10g11 (desmoglein 2) antibody - by Bioz Stars, 2026-02
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10g11-9h10 dvd-ig protein  (Nature Biotechnology)
90
Nature Biotechnology 10g11-9h10 dvd-ig protein
Cell–cell adhesion via E-cadherin and <t>desmoglein-2</t> occurs by a cooperative mechanism in both E-variants and TPA-treated R-variants. Monolayers of HCT-8/R1 cells ( R ) were treated with 250 ng/ml TPA as indicated in Materials and Methods. Monolayers of HCT-8/E8 cells ( E ) were not treated with TPA here ( b and d ), although analysis of TPA-treated E-variants yielded essentially identical data (not shown). Both R- and E-variants were analyzed for cell aggregation (increasing particle volume) either after 0 min (broken lines in a and b ; peak positions indicated by 0-min arrows in c and d ) or after 30 min of incubation at 37°C (full lines in all panels). Aggregation for 30 min by R-variants, which were not treated with TPA, yielded curves coinciding with the 0-min curves (not shown). Other curves depicted correspond with the following antibody treatments (see also Materials and Methods). The mAb MB-2 against human E-cadherin (O) was used at an effective concentration (dilution 1:20; a and b ), or at a more ineffective concentration (1:200; c and d , with in c only peak position indicated by an arrow). The mAb <t>10G11</t> against desmoglein-2 (□) was also used at an effective concentration (1:2; a and b ), or at an ineffective concentration (1:8; c and d , with in d only peak position indicated by an arrow). These two antibodies, both at the lower concentrations, were also combined as indicated by filled circles (•) in c and d. Upon such combination, a clear-cut synergistic inhibition of cell aggregation was observed for both cell types.
10g11 9h10 Dvd Ig Protein, supplied by Nature Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/10g11-9h10 dvd-ig protein/product/Nature Biotechnology
Average 90 stars, based on 1 article reviews
10g11-9h10 dvd-ig protein - by Bioz Stars, 2026-02
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cd49b clone 10g11  (Sanquin)
90
Sanquin cd49b clone 10g11
Cell–cell adhesion via E-cadherin and <t>desmoglein-2</t> occurs by a cooperative mechanism in both E-variants and TPA-treated R-variants. Monolayers of HCT-8/R1 cells ( R ) were treated with 250 ng/ml TPA as indicated in Materials and Methods. Monolayers of HCT-8/E8 cells ( E ) were not treated with TPA here ( b and d ), although analysis of TPA-treated E-variants yielded essentially identical data (not shown). Both R- and E-variants were analyzed for cell aggregation (increasing particle volume) either after 0 min (broken lines in a and b ; peak positions indicated by 0-min arrows in c and d ) or after 30 min of incubation at 37°C (full lines in all panels). Aggregation for 30 min by R-variants, which were not treated with TPA, yielded curves coinciding with the 0-min curves (not shown). Other curves depicted correspond with the following antibody treatments (see also Materials and Methods). The mAb MB-2 against human E-cadherin (O) was used at an effective concentration (dilution 1:20; a and b ), or at a more ineffective concentration (1:200; c and d , with in c only peak position indicated by an arrow). The mAb <t>10G11</t> against desmoglein-2 (□) was also used at an effective concentration (1:2; a and b ), or at an ineffective concentration (1:8; c and d , with in d only peak position indicated by an arrow). These two antibodies, both at the lower concentrations, were also combined as indicated by filled circles (•) in c and d. Upon such combination, a clear-cut synergistic inhibition of cell aggregation was observed for both cell types.
Cd49b Clone 10g11, supplied by Sanquin, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd49b clone 10g11/product/Sanquin
Average 90 stars, based on 1 article reviews
cd49b clone 10g11 - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


Cell–cell adhesion via E-cadherin and desmoglein-2 occurs by a cooperative mechanism in both E-variants and TPA-treated R-variants. Monolayers of HCT-8/R1 cells ( R ) were treated with 250 ng/ml TPA as indicated in Materials and Methods. Monolayers of HCT-8/E8 cells ( E ) were not treated with TPA here ( b and d ), although analysis of TPA-treated E-variants yielded essentially identical data (not shown). Both R- and E-variants were analyzed for cell aggregation (increasing particle volume) either after 0 min (broken lines in a and b ; peak positions indicated by 0-min arrows in c and d ) or after 30 min of incubation at 37°C (full lines in all panels). Aggregation for 30 min by R-variants, which were not treated with TPA, yielded curves coinciding with the 0-min curves (not shown). Other curves depicted correspond with the following antibody treatments (see also Materials and Methods). The mAb MB-2 against human E-cadherin (O) was used at an effective concentration (dilution 1:20; a and b ), or at a more ineffective concentration (1:200; c and d , with in c only peak position indicated by an arrow). The mAb 10G11 against desmoglein-2 (□) was also used at an effective concentration (1:2; a and b ), or at an ineffective concentration (1:8; c and d , with in d only peak position indicated by an arrow). These two antibodies, both at the lower concentrations, were also combined as indicated by filled circles (•) in c and d. Upon such combination, a clear-cut synergistic inhibition of cell aggregation was observed for both cell types.

Journal: The Journal of Cell Biology

Article Title: Protein Kinase C Activation Upregulates Intercellular Adhesion of α-Catenin–negative Human Colon Cancer Cell Variants via Induction of Desmosomes

doi:

Figure Lengend Snippet: Cell–cell adhesion via E-cadherin and desmoglein-2 occurs by a cooperative mechanism in both E-variants and TPA-treated R-variants. Monolayers of HCT-8/R1 cells ( R ) were treated with 250 ng/ml TPA as indicated in Materials and Methods. Monolayers of HCT-8/E8 cells ( E ) were not treated with TPA here ( b and d ), although analysis of TPA-treated E-variants yielded essentially identical data (not shown). Both R- and E-variants were analyzed for cell aggregation (increasing particle volume) either after 0 min (broken lines in a and b ; peak positions indicated by 0-min arrows in c and d ) or after 30 min of incubation at 37°C (full lines in all panels). Aggregation for 30 min by R-variants, which were not treated with TPA, yielded curves coinciding with the 0-min curves (not shown). Other curves depicted correspond with the following antibody treatments (see also Materials and Methods). The mAb MB-2 against human E-cadherin (O) was used at an effective concentration (dilution 1:20; a and b ), or at a more ineffective concentration (1:200; c and d , with in c only peak position indicated by an arrow). The mAb 10G11 against desmoglein-2 (□) was also used at an effective concentration (1:2; a and b ), or at an ineffective concentration (1:8; c and d , with in d only peak position indicated by an arrow). These two antibodies, both at the lower concentrations, were also combined as indicated by filled circles (•) in c and d. Upon such combination, a clear-cut synergistic inhibition of cell aggregation was observed for both cell types.

Article Snippet: The following mouse monoclonal antibodies were used: antibodies HECD-1 (Takara Biochemicals, Otsu, Japan) and MB2 ( ) both functionally blocking human E-cadherin, 1G5 against α-catenin ( Becton Dickinson , San Jose, CA), anti–β-catenin against β-catenin (Transduction Laboratories, Lexington, KY), anti-pp120 against p120 cas (Transduction Laboratories), PG 5.1 against plakoglobin and DG 3.10 against desmoglein (both from Cymbus Bioscience Ltd., Southampton, UK), 10G11 against desmoglein-2 (Progen, Heidelberg, Germany), DP 2.15 against desmoplakin I and II ( Boehringer Mannheim , Mannheim, Germany), hVIN-1 against vinculin ( Sigma Immunochemicals, St. Louis, MO), and htr-5 against the human TNF-receptor p55 ( ).

Techniques: Incubation, Concentration Assay, Inhibition

No major effect on E-cadherin, but formation of typical tight junctions and spot desmosomes upon TPA treatment of α-catenin–negative variants of HCT-8. Cells were double stained for E-cadherin ( a–c ) and occludin ( d–f ), or stained for desmoglein-2 ( g–i ). Each panel comprises an extended depth view by overlaying several confocal X-Y sections ( top ) and confocal X-Z sections of the same field ( bottom ). Cells analyzed were: epithelioid HCT-8/E8 cells ( a , d , g ), untreated HCT-8/R1 cells ( b , e , h ), and TPA-treated (250 ng/ml for 3 h) HCT-8/R1 cells ( c , f , i ). Bar, 14 μm.

Journal: The Journal of Cell Biology

Article Title: Protein Kinase C Activation Upregulates Intercellular Adhesion of α-Catenin–negative Human Colon Cancer Cell Variants via Induction of Desmosomes

doi:

Figure Lengend Snippet: No major effect on E-cadherin, but formation of typical tight junctions and spot desmosomes upon TPA treatment of α-catenin–negative variants of HCT-8. Cells were double stained for E-cadherin ( a–c ) and occludin ( d–f ), or stained for desmoglein-2 ( g–i ). Each panel comprises an extended depth view by overlaying several confocal X-Y sections ( top ) and confocal X-Z sections of the same field ( bottom ). Cells analyzed were: epithelioid HCT-8/E8 cells ( a , d , g ), untreated HCT-8/R1 cells ( b , e , h ), and TPA-treated (250 ng/ml for 3 h) HCT-8/R1 cells ( c , f , i ). Bar, 14 μm.

Article Snippet: The following mouse monoclonal antibodies were used: antibodies HECD-1 (Takara Biochemicals, Otsu, Japan) and MB2 ( ) both functionally blocking human E-cadherin, 1G5 against α-catenin ( Becton Dickinson , San Jose, CA), anti–β-catenin against β-catenin (Transduction Laboratories, Lexington, KY), anti-pp120 against p120 cas (Transduction Laboratories), PG 5.1 against plakoglobin and DG 3.10 against desmoglein (both from Cymbus Bioscience Ltd., Southampton, UK), 10G11 against desmoglein-2 (Progen, Heidelberg, Germany), DP 2.15 against desmoplakin I and II ( Boehringer Mannheim , Mannheim, Germany), hVIN-1 against vinculin ( Sigma Immunochemicals, St. Louis, MO), and htr-5 against the human TNF-receptor p55 ( ).

Techniques: Staining

Relocalization of desmosomal proteins to cell–cell contacts upon TPA treatment of α-catenin–negative variants of HCT-8. Cells were stained for plakoglobin ( a , b , and c ), desmoglein-2 ( d , e , and f ), or desmoplakin ( g , h , and i ). In the untreated epithelioid variant HCT-8/E8, desmosomal proteins were localized at the cell–cell contacts ( a , d , and g ). HCT-8/R1 cultures were either untreated ( b , e , and h ) or treated for 3 h with 250 ng/ml TPA ( c , f , and i ). Similar observations were done for other R-variants of HCT-8. Bar, 10 μm.

Journal: The Journal of Cell Biology

Article Title: Protein Kinase C Activation Upregulates Intercellular Adhesion of α-Catenin–negative Human Colon Cancer Cell Variants via Induction of Desmosomes

doi:

Figure Lengend Snippet: Relocalization of desmosomal proteins to cell–cell contacts upon TPA treatment of α-catenin–negative variants of HCT-8. Cells were stained for plakoglobin ( a , b , and c ), desmoglein-2 ( d , e , and f ), or desmoplakin ( g , h , and i ). In the untreated epithelioid variant HCT-8/E8, desmosomal proteins were localized at the cell–cell contacts ( a , d , and g ). HCT-8/R1 cultures were either untreated ( b , e , and h ) or treated for 3 h with 250 ng/ml TPA ( c , f , and i ). Similar observations were done for other R-variants of HCT-8. Bar, 10 μm.

Article Snippet: The following mouse monoclonal antibodies were used: antibodies HECD-1 (Takara Biochemicals, Otsu, Japan) and MB2 ( ) both functionally blocking human E-cadherin, 1G5 against α-catenin ( Becton Dickinson , San Jose, CA), anti–β-catenin against β-catenin (Transduction Laboratories, Lexington, KY), anti-pp120 against p120 cas (Transduction Laboratories), PG 5.1 against plakoglobin and DG 3.10 against desmoglein (both from Cymbus Bioscience Ltd., Southampton, UK), 10G11 against desmoglein-2 (Progen, Heidelberg, Germany), DP 2.15 against desmoplakin I and II ( Boehringer Mannheim , Mannheim, Germany), hVIN-1 against vinculin ( Sigma Immunochemicals, St. Louis, MO), and htr-5 against the human TNF-receptor p55 ( ).

Techniques: Staining, Variant Assay

TPA induces exposition of desmoglein-2 molecules at the cell surface in α-catenin–negative variants of HCT-8. Monolayers of the epithelioid HCT-8/E8 ( E ) and of the round cell variant HCT-8/R1 ( R ) were treated for the indicated times with 250 ng/ml TPA, and then labeled with NHS-biotin for 30 min and extracted with lysis buffer. Biotinylated proteins were purified by adsorbing to avidin-agarose beads and analyzed by Western blotting using antibodies to E-cadherin (HECD-1) or desmoglein (DG 3.10).

Journal: The Journal of Cell Biology

Article Title: Protein Kinase C Activation Upregulates Intercellular Adhesion of α-Catenin–negative Human Colon Cancer Cell Variants via Induction of Desmosomes

doi:

Figure Lengend Snippet: TPA induces exposition of desmoglein-2 molecules at the cell surface in α-catenin–negative variants of HCT-8. Monolayers of the epithelioid HCT-8/E8 ( E ) and of the round cell variant HCT-8/R1 ( R ) were treated for the indicated times with 250 ng/ml TPA, and then labeled with NHS-biotin for 30 min and extracted with lysis buffer. Biotinylated proteins were purified by adsorbing to avidin-agarose beads and analyzed by Western blotting using antibodies to E-cadherin (HECD-1) or desmoglein (DG 3.10).

Article Snippet: The following mouse monoclonal antibodies were used: antibodies HECD-1 (Takara Biochemicals, Otsu, Japan) and MB2 ( ) both functionally blocking human E-cadherin, 1G5 against α-catenin ( Becton Dickinson , San Jose, CA), anti–β-catenin against β-catenin (Transduction Laboratories, Lexington, KY), anti-pp120 against p120 cas (Transduction Laboratories), PG 5.1 against plakoglobin and DG 3.10 against desmoglein (both from Cymbus Bioscience Ltd., Southampton, UK), 10G11 against desmoglein-2 (Progen, Heidelberg, Germany), DP 2.15 against desmoplakin I and II ( Boehringer Mannheim , Mannheim, Germany), hVIN-1 against vinculin ( Sigma Immunochemicals, St. Louis, MO), and htr-5 against the human TNF-receptor p55 ( ).

Techniques: Variant Assay, Labeling, Lysis, Purification, Avidin-Biotin Assay, Western Blot

TPA does not modify the detergent solubility of E-cadherin, β-catenin, plakoglobin, and desmoglein-2. Monolayers of HCT-8/E8 ( E ) and HCT-8/R1 ( R ) were either untreated or treated with 250 ng/ml TPA for 3 h as indicated. Detergent (2.5% NP-40) soluble and insoluble fractions were prepared and analyzed by Western blotting using antibodies to the antigens indicated at the right.

Journal: The Journal of Cell Biology

Article Title: Protein Kinase C Activation Upregulates Intercellular Adhesion of α-Catenin–negative Human Colon Cancer Cell Variants via Induction of Desmosomes

doi:

Figure Lengend Snippet: TPA does not modify the detergent solubility of E-cadherin, β-catenin, plakoglobin, and desmoglein-2. Monolayers of HCT-8/E8 ( E ) and HCT-8/R1 ( R ) were either untreated or treated with 250 ng/ml TPA for 3 h as indicated. Detergent (2.5% NP-40) soluble and insoluble fractions were prepared and analyzed by Western blotting using antibodies to the antigens indicated at the right.

Article Snippet: The following mouse monoclonal antibodies were used: antibodies HECD-1 (Takara Biochemicals, Otsu, Japan) and MB2 ( ) both functionally blocking human E-cadherin, 1G5 against α-catenin ( Becton Dickinson , San Jose, CA), anti–β-catenin against β-catenin (Transduction Laboratories, Lexington, KY), anti-pp120 against p120 cas (Transduction Laboratories), PG 5.1 against plakoglobin and DG 3.10 against desmoglein (both from Cymbus Bioscience Ltd., Southampton, UK), 10G11 against desmoglein-2 (Progen, Heidelberg, Germany), DP 2.15 against desmoplakin I and II ( Boehringer Mannheim , Mannheim, Germany), hVIN-1 against vinculin ( Sigma Immunochemicals, St. Louis, MO), and htr-5 against the human TNF-receptor p55 ( ).

Techniques: Solubility, Western Blot