Journal: BMC ophthalmology

Article Title: Proteomic analysis of retinal pigment epithelium cells after exposure to UVA radiation.

doi: 10.1186/s12886-019-1151-9

Figure Lengend Snippet: Fig. 3 Identification of the differentially expressed proteins between the control and UVA irradiated ARPE-19 cells by 2-DE. a ARPE-19 cells irradiated with 10 J/cm2 UVA, followed by harvesting the cells and cell lysates. A total of 300 μg protein was then subjected to 2-DE and visualized by CBR staining. PDQuest image analysis software (Bio-Rad) was employed to explore differential expression through differential protein spotting. b Validation of identified selected proteins from 2-DE by western blot analysis. Some identified proteins include GRP78, CALR, PDI, NPM, Hsp27, ATP synthase, GAPDH, and PRDX1. β-actin was used as the loading control

Article Snippet: We purchased antibodies against Heat shock protein 27 (Hsp27), nucleophosmin (NPM), ATP synthase, and GAPDH from Epitomics (Burlingame, CA, USA) and antibodies against glucose-related protein 78 (GRP78), calreticulin (CALR), protein disulfide isomerase (PDI), cleaved-ATF6, ATF4, and PRDX1 antibodies were purchased from ProteinTech Group (Chicago, IL, USA).

Techniques: Control, Irradiation, Staining, Software, Quantitative Proteomics, Biomarker Discovery, Western Blot

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Asiaticoside Combined With Carbon Ion Implantation to Improve the Biocompatibility of Silicone Rubber and to Reduce the Risk of Capsule Contracture

doi: 10.3389/fbioe.2022.810244

Figure Lengend Snippet: Effect of asiaticoside on TGF-β1-induced transformation of fibroblasts into myofibroblasts and collagen deposition. Immunofluorescence staining of human fibroblasts on silicone rubber (SR) and carbon silicone rubber (C-SR) with Col-1A1 and α-SMA (100X).

Article Snippet: The primary antibodies were as follows: Smad2/3 (1:500, Affinity, United States), vimentin (1:500, Proteintech, China), α-SMA (1:500, Proteintech, China), Col-1A1 (1:500, Novus, United States), TGF-β1 (1:500, Affinity, United States), TβRI (1:500, Abcam, United States), TβRII (1:500, Abcam, United States), Smad2/3 (1:500, Affinity, United States), p-Smad2/3 (1:500, Affinity, United States), and β-actin (1:1,000, Abcam, United States).

Techniques: Transformation Assay, Immunofluorescence, Staining

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Asiaticoside Combined With Carbon Ion Implantation to Improve the Biocompatibility of Silicone Rubber and to Reduce the Risk of Capsule Contracture

doi: 10.3389/fbioe.2022.810244

Figure Lengend Snippet: Effect of asiaticoside on transformation of fibroblasts into myofibroblasts, collagen deposition and changes in the TGF-β/Smad signaling pathway in vivo . (A) Representative Western blot analysis of TGF-β1, TβRI, TβRII, Col-1A1, Smad2/3, p-Smad2/3 and α-SMA. One representative blot of three experiments is presented. β-actin was used as loading control. (B–G) Protein signals in A were determined by densitometric analysis of Western blotting using ImageJ software (NIH). Data are reported as the mean ± standard error ( n = 3, ∗ p < 0.05).

Article Snippet: The primary antibodies were as follows: Smad2/3 (1:500, Affinity, United States), vimentin (1:500, Proteintech, China), α-SMA (1:500, Proteintech, China), Col-1A1 (1:500, Novus, United States), TGF-β1 (1:500, Affinity, United States), TβRI (1:500, Abcam, United States), TβRII (1:500, Abcam, United States), Smad2/3 (1:500, Affinity, United States), p-Smad2/3 (1:500, Affinity, United States), and β-actin (1:1,000, Abcam, United States).

Techniques: Transformation Assay, In Vivo, Western Blot, Control, Software

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Asiaticoside Combined With Carbon Ion Implantation to Improve the Biocompatibility of Silicone Rubber and to Reduce the Risk of Capsule Contracture

doi: 10.3389/fbioe.2022.810244

Figure Lengend Snippet: Effect of asiaticoside on the transformation of fibroblasts into myofibroblasts, collagen deposition and fibroblast proliferation in vivo . Immunohistochemical staining of tissue around silicone rubber (SR) and carbon silicone rubber (C-SR) with α-SMA, vimentin, PCNA and COL-1A1 (100X).

Article Snippet: The primary antibodies were as follows: Smad2/3 (1:500, Affinity, United States), vimentin (1:500, Proteintech, China), α-SMA (1:500, Proteintech, China), Col-1A1 (1:500, Novus, United States), TGF-β1 (1:500, Affinity, United States), TβRI (1:500, Abcam, United States), TβRII (1:500, Abcam, United States), Smad2/3 (1:500, Affinity, United States), p-Smad2/3 (1:500, Affinity, United States), and β-actin (1:1,000, Abcam, United States).

Techniques: Transformation Assay, In Vivo, Immunohistochemical staining, Staining

Journal: RSC Advances

Article Title: Thymoquinone protects against cardiac damage from doxorubicin-induced heart failure in Sprague-Dawley rats

doi: 10.1039/c8ra00975a

Figure Lengend Snippet: Fig. 4 Protein levels of TGF-b, Smad3, collagen I, collagen III, and a-SMA in cardiac tissue were determined by western blotting. Immunoblotting for TGF-b, Smad3, collagen I, collagen III, and a-SMA in cardiac tissues. Bar graph showing quantification of TGF-b, Smad3, collagen I, collagen III, and a-SMA protein expression. Data are given as the mean SEM; n ¼ 3 in each group. *p < 0.05 vs. DOX group.

Article Snippet: Primary antibodies against TGF-b (rabbit anti-TGF-b antibody, 1 : 1000; Proteintech, Wuhan, China), Smad3 (rabbit anti-Smad antibody, 1 : 1000; Proteintech), collagen I (rabbit anti-collagen I antibody, 1 : 1000; Proteintech), collagen III (rabbit anticollagen III antibody, 1 : 1000; Proteintech), a-SMA (rabbit anti-a-SMA antibody, 1 : 1000; Proteintech), P53 (rabbit antiP53 antibody, 1 : 1000; Proteintech), bcl-2(rabbit anti-bcl-2 antibody, 1 : 1000; Proteintech), anti-b-actin (1 : 1000; Cell Signaling Technology).

Techniques: Western Blot, Expressing

Journal: Acta Neuropathologica Communications

Article Title: C-terminal and full length TDP-43 specie differ according to FTLD-TDP lesion type but not genetic mutation

doi: 10.1186/s40478-019-0755-x

Figure Lengend Snippet: The amino acid sequence of TDP-43 from N terminus (1) to C terminus (414). The highlighted regions are the peptides used to generate the polyclonal antibodies (MC2079, MC2085 and pTDP 409/410)

Article Snippet: In all 24 cases we performed serial sectioning and TDP-43 immunohistochemistry with four different TDP-43 antibodies: phosphorylated TDP-43 (pTDP-43) antibody (pS409/410, 1:5000 mouse monoclonal, Cosmo Bio Co., LTD) that recognizes TDP-43 with phosphorylated epitopes; anti TDP-43 antibody that recognizes a neoepitope in the C terminal fragment of cleaved TDP-43(cTDP-43) (MC2085, 1:2500 rabbit polyclonal, gift from Leonard Petrucelli, Mayo Clinic) [ ]; anti TDP-43 antibody that recognizes an epitope in the amino terminus (nTDP-43) (MC1079; 1:2500 rabbit polyclonal, gift from Leonard Petrucelli, Mayo Clinic) [ ] and a rabbit polyclonal anti-TDP-43 antibody (ProteinTech Group; Chicago IL; dilution 1:3000) which is a commercially available antibody.

Techniques: Sequencing