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tanespimycin 17 aag  (MedChemExpress)
96
MedChemExpress tanespimycin 17 aag
BBR did not inhibit Hsp90 and HDACs in SW480 cells. (A) The binding effect between BBR (100 μM) and Hsp90 was detected by the thermal stabilization-based CESTA method at 48–76 °C after 30 min of incubation at room temperature. (B) Hsp90 protein expression was quantified and the data are presented as means ± S.D. of three independent experiments. (C) Western blot analysis of Hsp70, IKKβ and EGFR in BBR treated SW480 cells after 24 h incubation. The Hsp90 <t>inhibitor</t> <t>17-AAG</t> was included as positive control. (D) Western blot analysis of HDAC proteins in BBR treated SW480 cells after 24 h incubation.
Tanespimycin 17 Aag, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BBR did not inhibit Hsp90 and HDACs in SW480 cells. (A) The binding effect between BBR (100 μM) and Hsp90 was detected by the thermal stabilization-based CESTA method at 48–76 °C after 30 min of incubation at room temperature. (B) Hsp90 protein expression was quantified and the data are presented as means ± S.D. of three independent experiments. (C) Western blot analysis of Hsp70, IKKβ and EGFR in BBR treated SW480 cells after 24 h incubation. The Hsp90 inhibitor 17-AAG was included as positive control. (D) Western blot analysis of HDAC proteins in BBR treated SW480 cells after 24 h incubation.

Journal: Pharmaceutical Biology

Article Title: Berberine regulates the Notch1/PTEN/PI3K/AKT/mTOR pathway and acts synergistically with 17-AAG and SAHA in SW480 colon cancer cells

doi: 10.1080/13880209.2020.1865407

Figure Lengend Snippet: BBR did not inhibit Hsp90 and HDACs in SW480 cells. (A) The binding effect between BBR (100 μM) and Hsp90 was detected by the thermal stabilization-based CESTA method at 48–76 °C after 30 min of incubation at room temperature. (B) Hsp90 protein expression was quantified and the data are presented as means ± S.D. of three independent experiments. (C) Western blot analysis of Hsp70, IKKβ and EGFR in BBR treated SW480 cells after 24 h incubation. The Hsp90 inhibitor 17-AAG was included as positive control. (D) Western blot analysis of HDAC proteins in BBR treated SW480 cells after 24 h incubation.

Article Snippet: Tanespimycin (17-AAG) and Vorinostat (SAHA) were from Medchem Express (Monmouth Junction, NJ).

Techniques: Binding Assay, Incubation, Expressing, Western Blot, Positive Control

BBR enhanced the anti-proliferative activity of 17-AAG and SAHA, and the combination of the three has more potent synergistic effect on SW480 cells. The incubation time of SW480 cells and the test drugs was 24 h. (A) SW480 cells were exposed to specified concentrations of 17-AAG for 24 h in the presence of 0–2 µM of BBR. (B) The CI values of BBR and 17-AAG combinations. (C) SW480 cells were exposed to specified concentrations of SAHA for 24 h in the of 0–2 µM of BBR. (D) The CI values of BBR and SAHA combinations. (E) SW480 cells were treated with specified concentrations of 17-AAG and SAHA in the presence of 0–2 µM of BBR. (F) The CI values for BBR, 17-AAG and SAHA combinations. The above data are all presented as the means ± S.D. of three independent experiments. * p < 0.05, ** p < 0.01 when comparing with 17-AAG, SAHA and both 17-AAG and SAHA treatment in the absence of BBR.

Journal: Pharmaceutical Biology

Article Title: Berberine regulates the Notch1/PTEN/PI3K/AKT/mTOR pathway and acts synergistically with 17-AAG and SAHA in SW480 colon cancer cells

doi: 10.1080/13880209.2020.1865407

Figure Lengend Snippet: BBR enhanced the anti-proliferative activity of 17-AAG and SAHA, and the combination of the three has more potent synergistic effect on SW480 cells. The incubation time of SW480 cells and the test drugs was 24 h. (A) SW480 cells were exposed to specified concentrations of 17-AAG for 24 h in the presence of 0–2 µM of BBR. (B) The CI values of BBR and 17-AAG combinations. (C) SW480 cells were exposed to specified concentrations of SAHA for 24 h in the of 0–2 µM of BBR. (D) The CI values of BBR and SAHA combinations. (E) SW480 cells were treated with specified concentrations of 17-AAG and SAHA in the presence of 0–2 µM of BBR. (F) The CI values for BBR, 17-AAG and SAHA combinations. The above data are all presented as the means ± S.D. of three independent experiments. * p < 0.05, ** p < 0.01 when comparing with 17-AAG, SAHA and both 17-AAG and SAHA treatment in the absence of BBR.

Article Snippet: Tanespimycin (17-AAG) and Vorinostat (SAHA) were from Medchem Express (Monmouth Junction, NJ).

Techniques: Activity Assay, Incubation