γklotho Search Results


γklotho  (R&D Systems)
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A soluble form of αKlotho downregulates SOCE without affecting Orai1/STIM1. a Representative trace showing the effect of the <t>Klotho</t> family on SOCE. Flag-Orai1 and YFP-STIM1 co-transfected with Klotho isoforms: αKlotho (KLA), βKlotho (KLB), or γKlotho (KLG) in HEK293FT cells. Empty vector (pEF1 vector) used as transfection control. b Quantification of peak SOCE values is expressed as mean ± SEM ( n = 55–173 each group). c Immunoblotting showing transfection of Klotho isoforms and Flag-tagged Orai1 and YFP-tagged STIM1. d Quantitative real-time PCR for relative mRNA expression of Orais and STIMs in HEK293FT cells. e Representative trace of endogenous SOCE in HEK293FT cells transiently expressing full-length (KLFL) and secreted (KLΔTM) form of αKlotho. Empty vector (pEF1 vector) was used as a transfection control (Vector). f Summary of the SOCE in panel e ( n = 123–186 each group). g Effect of αKlotho (KLFL and KLΔTM) on endogenous Orai1 and STIM1 protein expression in HEK293FT cells. **Denotes p < 0.01. Data were analyzed by one-way ANOVA ( b left panel in d and f ) and t test (right panel in d)
γklotho, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A soluble form of αKlotho downregulates SOCE without affecting Orai1/STIM1. a Representative trace showing the effect of the Klotho family on SOCE. Flag-Orai1 and YFP-STIM1 co-transfected with Klotho isoforms: αKlotho (KLA), βKlotho (KLB), or γKlotho (KLG) in HEK293FT cells. Empty vector (pEF1 vector) used as transfection control. b Quantification of peak SOCE values is expressed as mean ± SEM ( n = 55–173 each group). c Immunoblotting showing transfection of Klotho isoforms and Flag-tagged Orai1 and YFP-tagged STIM1. d Quantitative real-time PCR for relative mRNA expression of Orais and STIMs in HEK293FT cells. e Representative trace of endogenous SOCE in HEK293FT cells transiently expressing full-length (KLFL) and secreted (KLΔTM) form of αKlotho. Empty vector (pEF1 vector) was used as a transfection control (Vector). f Summary of the SOCE in panel e ( n = 123–186 each group). g Effect of αKlotho (KLFL and KLΔTM) on endogenous Orai1 and STIM1 protein expression in HEK293FT cells. **Denotes p < 0.01. Data were analyzed by one-way ANOVA ( b left panel in d and f ) and t test (right panel in d)

Journal: Pflugers Archiv

Article Title: Soluble αKlotho downregulates Orai1-mediated store-operated Ca 2+ entry via PI3K-dependent signaling

doi: 10.1007/s00424-020-02510-1

Figure Lengend Snippet: A soluble form of αKlotho downregulates SOCE without affecting Orai1/STIM1. a Representative trace showing the effect of the Klotho family on SOCE. Flag-Orai1 and YFP-STIM1 co-transfected with Klotho isoforms: αKlotho (KLA), βKlotho (KLB), or γKlotho (KLG) in HEK293FT cells. Empty vector (pEF1 vector) used as transfection control. b Quantification of peak SOCE values is expressed as mean ± SEM ( n = 55–173 each group). c Immunoblotting showing transfection of Klotho isoforms and Flag-tagged Orai1 and YFP-tagged STIM1. d Quantitative real-time PCR for relative mRNA expression of Orais and STIMs in HEK293FT cells. e Representative trace of endogenous SOCE in HEK293FT cells transiently expressing full-length (KLFL) and secreted (KLΔTM) form of αKlotho. Empty vector (pEF1 vector) was used as a transfection control (Vector). f Summary of the SOCE in panel e ( n = 123–186 each group). g Effect of αKlotho (KLFL and KLΔTM) on endogenous Orai1 and STIM1 protein expression in HEK293FT cells. **Denotes p < 0.01. Data were analyzed by one-way ANOVA ( b left panel in d and f ) and t test (right panel in d)

Article Snippet: Primary antibodies were used following as: Orai1 (HPA016583, ATLAS antibodies, Stockholm, Sweden), STIM1 (11565-1-AP, ProteinTech Group Inc., Chicago, IL, USA), GFP (ab137687, Abcam, Cambridge, UK), αKlotho (clone KM2076, KAL-KO603, Cosmo Bio Co., Ltd., Tokyo, Japan), βKlotho (GTX45558, Gene Tex, Inc., Irvine, CA), γKlotho (AF5984-SP, R&D Systems, Minneapolis, MN, USA), Flag-HRP (A8592, Sigma-Aldrich, St. Louis, MO, USA), β-actin (ab6276, abcam, Cambridge, UK), p-Akt Ser407 (#9271), p-Akt Thr308 (#2965), and Akt (#9272) were provided from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Transfection, Plasmid Preparation, Control, Western Blot, Real-time Polymerase Chain Reaction, Expressing