Journal:

Article Title: Altered pharmacology and GABA-A receptor subunit expression in dorsal midline thalamic neurons in limbic epilepsy

doi: 10.1016/j.nbd.2008.09.023

Figure Lengend Snippet: Distribution of α1, β2/3, γ2, of the GABAAR, and GAD-IR in thalamus captured at low magnification (2x, NA = 0.1) to demonstrate different intensity of staining at different areas. The white arrows point to PV and the white arrowheads point to MD. The α1, β2/3, γ2, and GAD-IR were expressed in most thalamic nuclei, and the entire thalamus appeared uniformly stained for these subunits, except for the reticular nucleus and the habenula or hippocampus (A–D). The staining was diffuse, and individual neurons could not be readily distinguished, except that of α1-IR in hippocampal dentate gyrus (A). The scale bar in figure A is 200 µm, and also applies to B, C and D.

Article Snippet: Antibodies and image acquisition The antibodies against the GABAAR subunit α1 (1–16) and γ2 (1–33) were obtained from Alomone labs (Israel).

Techniques: Staining

Journal:

Article Title: Altered pharmacology and GABA-A receptor subunit expression in dorsal midline thalamic neurons in limbic epilepsy

doi: 10.1016/j.nbd.2008.09.023

Figure Lengend Snippet: High magnification (63X) images obtained with laser scanning confocal microscopy reveal the diffuse distribution of GABAAR α1, β2/3, and γ2 subunits and GAD65 immunoreactivity in control MD. The distribution of IR of α1 (A), β2/3 (B), and γ2 (C) subunits was similar and found to be present as many small diffuse clusters and some large clusters. The immunoreactivity of GAD65 (D) was mainly found as large intense clusters. The scale bar in figure A is 7 µm.

Article Snippet: Antibodies and image acquisition The antibodies against the GABAAR subunit α1 (1–16) and γ2 (1–33) were obtained from Alomone labs (Israel).

Techniques: Confocal Microscopy