Journal: International Journal of Molecular Sciences

Article Title: Apheresis Platelet Rich-Plasma for Regenerative Medicine: An In Vitro Study on Osteogenic Potential

doi: 10.3390/ijms22168764

Figure Lengend Snippet: PRP dilution in the culture medium, and corresponding GFs concentration, used to treat HOB-01-C1 cells. Data are expressed as percentage volume in the culture medium for PRP dilution and mean ± SD for GFs concentration.

Article Snippet: The day after, the culture medium was replaced with an osteogenic medium composed as follows: MEM ALPHA MEDIUM (EUROCLONE SpA, Milan, Italy) supplemented with 0.1% Human Serum AB (EUROCLONE SpA, Milan, Italy), 200 mM Glutamine, 1%, Streptomycin/Penicillin, 100 μM ascorbic acid, 2.5 mM β-glycerolphosphate and 40 μg/mL L-proline.

Techniques: Concentration Assay

Journal: Stem Cells International

Article Title: Expression of CD24 in Human Bone Marrow-Derived Mesenchymal Stromal Cells Is Regulated by TGF β 3 and Induces a Myofibroblast-Like Genotype

doi: 10.1155/2016/1319578

Figure Lengend Snippet: IPA upstream regulator analysis. Molecules resulting in similar changes in the pattern of gene expression as the overexpression of CD24 or the knockdown of CD24 are designated with high activational z -score values. Listed are those molecules with a z -score higher than 2 while exhibiting opposing algebraic signs in the CD24 Up and CD24 Down Groups.

Article Snippet: Alternative culture medium to verify whether CD24 expression was not an artifact induced by FGF-2 or FBS consisted of alpha-medium (F0925, Biochrom), 10% AB-Human Serum (Life Technologies, Carlsbad, California, USA), 1% (100 U/mL/100 μ g/mL) penicillin/streptomycin, and 1% glutamine [ ].

Techniques: Gene Expression, Over Expression, Knockdown, Infection, Membrane, Activation Assay

Journal: Stem Cells International

Article Title: Expression of CD24 in Human Bone Marrow-Derived Mesenchymal Stromal Cells Is Regulated by TGF β 3 and Induces a Myofibroblast-Like Genotype

doi: 10.1155/2016/1319578

Figure Lengend Snippet: Expression of alpha smooth muscle actin. Intracellular immunocytochemical staining of alpha smooth muscle actin after overexpression of CD24 (CD24, left column) or truncated CD34 as control (CD34, right column). Alpha smooth muscle actin: staining of alpha smooth muscle actin (green), and nuclear staining (blue) show that in hBMSCs overexpressing CD24 reactivity for alpha smooth muscle actin increases relative to control cells (Scale 50 μ m). CD24: staining of CD24 (green), and nuclear staining (blue) verify that transduction rate of CD24 is nearly complete (Scale 100 μ m). CD34: staining of CD34 (green), and nuclear staining (blue) verify that control cells were transduced efficiently with truncated CD34 (Scale 100 μ m). Secondary antibody control: staining control with secondary antibody only (green) and nuclear staining (blue) (Scale 50 μ m).

Article Snippet: Alternative culture medium to verify whether CD24 expression was not an artifact induced by FGF-2 or FBS consisted of alpha-medium (F0925, Biochrom), 10% AB-Human Serum (Life Technologies, Carlsbad, California, USA), 1% (100 U/mL/100 μ g/mL) penicillin/streptomycin, and 1% glutamine [ ].

Techniques: Expressing, Staining, Over Expression, Control, Transduction