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HiMedia Laboratories
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IonOptix
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Rolf C Hagen Inc
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Barents Group LLC
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Rolf C Hagen Inc
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Thermocare Inc
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Millar Inc
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Cryogenic Control Systems
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LiCONiC US Inc
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Image Search Results
Journal: Cell Biochemistry and Function
Article Title: Electrically stimulated in vitro heart cell mimic of acute exercise reveals novel immediate cellular responses to exercise: Reduced contractility and metabolism, but maintained calcium cycling and increased myofilament calcium sensitivity
doi: 10.1002/cbf.3847
Figure Lengend Snippet: Comparison of intracellular myofilament Ca 2+ sensitivity after protocols that mimic acute aerobic endurance exercise in cardiomyocytes by applying 30 min trains of 16 ms pulse‐width twitch‐stimulations at 3 Hz (acute exercise, EX) or control (CON) at 0.3 Hz in culture incubators (Krebs solution, 37°C, pH 7.3, CaCl 2 1.8 mM). (A) Permeabilization of cardiomyocytes over a range of saponin concentrations ([saponin]) in the presence of free Ca 2+ (Ca 2+ ‐ethylene glycol tetraacetic acid (CaEGTA), whereas absence of free Ca 2+ (EGTA) indicates nontoxic [saponin]; * p < .05 versus baseline (no saponin) ( n = 5 animals). (B) Myofilament Ca 2+ sensitivity over 0.03 µM (10% CaEGTA)–2.40 µM (90% CaEGTA) free Ca 2+ range to determine [Ca 2+ ] that induces contraction ( n = 5 animals); insert shows more detailed assessment in the range 0.40 µM (60% CaEGTA)–1.07 µM (80% CaEGTA); note x ‐axis has linear scale ( n = 5 animals). (C) Index of myofilament Ca 2+ sensitivity in acute exercise and control after 30 min stimulation, measured by proportion of contracted/relaxed cardiomyocytes; * p < .05 versus CON ( n = 8 animals). (D) Index of myofilament Ca 2+ sensitivity in acute exercise and control after 30 min stimulation, measured by proportion of contracted cardiomyocytes/total cells; * p < .05 versus CON ( n = 8 animals). (E) Free [Ca 2+ ] that evoked half‐maximal effect (EC 50 ) after acute exercise and control ( n = 8 animals).
Article Snippet: Acute exercise mimic of 3 Hz or control of 0.3 Hz was therefore applied as trains of 30 V, 16ms pulse‐width field twitch‐stimulations for 30 min in Krebs solution, 37°C, pH 7.3, CaCl 2 1.8 mM in
Techniques: Comparison, Control