Journal: iScience

Article Title: Mechanical activation of PIEZO1 drives AP-1–dependent PTGS2/PGE2 signaling and corneal inflammation

doi: 10.1016/j.isci.2026.115396

Figure Lengend Snippet: Mechanical stretching activates AP-1 signaling via the PIEZO1-Ca 2+ pathway (A) Flow cytometry measurement of the mean fluorescence intensity of Ca 2+ in cells from different treatment groups (Control, Stretch, Stretch+GsMTx4) using Fluo-4 AM probe staining. (B) Quantitative analysis of the mean fluorescence intensity of Ca 2+ in the Control, Stretch, and Stretch+GsMTx4 groups ( n = 3). (C) Analysis of calcium concentration in the control, stretch, and stretch+GsMTx4 groups (μg/mL, n = 4). (D) Real-time imaging of calcium-fluorescent live cells under different treatment conditions (Con, Con+Yoda1, and Ca 2+ free+Yoda1 groups) (scale bars, 77 μm). (E and F) Mean fluorescence intensity curves for the Con, Con+Yoda1, and Ca 2+ free+Yoda1 groups over time (black arrows indicate the starting points for the addition of different drugs), with a total recording time of 10 min ( n = 6). (G) Western blotting analysis was performed to detect the expression of phosphorylated AP-1 proteins in the Control, Stretch, and Stretch+ Ca 2+ free groups, with β-actin serving as a loading control. (H, I) Relative protein levels of p -JUN and p -FOS were quantified ( n = 3). Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 (one-way ANOVA).

Article Snippet: Yoda1 , MedChemExpress , CAT#HY-18723.

Techniques: Flow Cytometry, Fluorescence, Control, Staining, Concentration Assay, Imaging, Western Blot, Expressing

Journal: iScience

Article Title: Mechanical activation of PIEZO1 drives AP-1–dependent PTGS2/PGE2 signaling and corneal inflammation

doi: 10.1016/j.isci.2026.115396

Figure Lengend Snippet: Activation of PIEZO1 regulates human corneal fibroblast function through the Ca 2+ /PTGS2 axis (A) Changes in PTGS2 mRNA levels following Yoda1 treatment (10 μM) ( n = 3). (B and C) Immunofluorescence staining and quantification of mean fluorescence intensity for PTGS2 protein in the control, Yoda1, and Yoda1+Ca 2+ free groups ( n = 3; scale bars, 50 μm). (D) Cell scratch assay for the Con, Yoda1 (10 μM), and Yoda1+Celecoxib (10 μM) groups (scale bars, 50 μm). (E) Quantitative analysis of scratch area (n = 3–5). (F) Immunofluorescence staining of COL1 protein in the Con, stretch, and stretch+celecoxib groups (scale bars, 50 μm). (G) Quantification of COL1 fluorescence intensity ( n = 4). (H) EdU fluorescent staining of the Con, stretch, and stretch+celecoxib groups (scale bars, 100 μm). (I) Quantitative analysis of EdU mean fluorescence intensity ( n = 5). Data are presented as mean ± SD. ∗∗ p < 0.01 and ∗∗∗ p < 0.001, ns = not significant (Student’s t test or one-way ANOVA).

Article Snippet: Yoda1 , MedChemExpress , CAT#HY-18723.

Techniques: Activation Assay, Immunofluorescence, Staining, Fluorescence, Control, Wound Healing Assay

Journal: iScience

Article Title: Ether phospholipids modulate somatosensory responses by tuning multiple receptor functions in Drosophila

doi: 10.1016/j.isci.2026.115209

Figure Lengend Snippet: ePLs modify dPIEZO activity in Drosophila cells (A–C) Representative Fura-2 imaging data for dPIEZO in control S2R+ cells and cells supplemented with 100 μM 18-AG. A, Typical ratiometric images before (Basal, at 60 s) and after the application of 10 μM Yoda1 (Yoda1, at 360 s) or 5 μM ionomycin (Ionomycin, at 540 s) in dPIEZO expressing cells (left) and dPIEZO expressing cells supplemented with 100 μM 18-AG (right). B, C, Representative Ca 2+ level traces in dPIEZO expressing cells (B), and 18-AG supplemented dPIEZO expressing cells (C). Red traces indicate Yoda1 responding cells (Δratio > 2). (D, E) Proportion of cells responding to Yoda1 (Δratio > 2) (D) and maximum Δratio response to Yoda1 normalized by the ionomycin response (E) in mock-transfected cells (control; n = 10, 18-AG; n = 10) and dPIEZO expressing cells (control, n = 10, 18-AG, n = 10). Each point represents a biological replicate; 25–40 cells were analyzed in each assay. Data are presented as mean ± SEM. ∗ p < 0.05 and ∗∗ p < 0.01; Tukey’s test. (F, G) Representative traces of patch-clamp recordings of mechanical stimuli-evoked dPIEZO activation without (F) or with (G) 100 μM 18-AG. (H) Quantification of the peak current density. Data are presented as mean ± SEM. ∗∗ p < 0.01; Mann-Whitney U test. (I) Proportion of responding cells (current size > 5 pA).

Article Snippet: Yoda1 , Tocris , 5586.

Techniques: Activity Assay, Imaging, Control, Expressing, Transfection, Patch Clamp, Activation Assay, MANN-WHITNEY