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Physicochemical and mechanical characterization of the hydrogel formulations. A) Schematic illustration of the preparation of GA–Zn 2+ /BP@SilMA and incorporation of osteogenic bone microtissues (BO). B) Photographs of the hydrogel precursor before and after ultraviolet. C) Scanning electron microscopy (SEM) images of SilMA, GA–Zn 2+ @SilMA, GA–Zn 2+ /BP@SilMA, and GA–Zn 2+ /BP/BO@SilMA hydrogels. D) <t>Energy-dispersive</t> <t>X-ray</t> spectroscopy (EDS) elemental maps of C, O, P, and Zn in the composite hydrogel. E) Fourier-transform infrared spectroscopy (FTIR) spectra of the different hydrogel formulations. F) X-ray <t>diffraction</t> (XRD) patterns of the different hydrogel formulations. G) Atomic force microscopy (AFM) force–separation curves. H) Zn 2+ release profile. I) Degradation behavior of the hydrogel. J) Young's modulus of the different hydrogel formulations. Data are presented as mean ± SD. *p < 0.05 and ****p < 0.0001.
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Physicochemical and mechanical characterization of the hydrogel formulations. A) Schematic illustration of the preparation of GA–Zn 2+ /BP@SilMA and incorporation of osteogenic bone microtissues (BO). B) Photographs of the hydrogel precursor before and after ultraviolet. C) Scanning electron microscopy (SEM) images of SilMA, GA–Zn 2+ @SilMA, GA–Zn 2+ /BP@SilMA, and GA–Zn 2+ /BP/BO@SilMA hydrogels. D) <t>Energy-dispersive</t> <t>X-ray</t> spectroscopy (EDS) elemental maps of C, O, P, and Zn in the composite hydrogel. E) Fourier-transform infrared spectroscopy (FTIR) spectra of the different hydrogel formulations. F) X-ray <t>diffraction</t> (XRD) patterns of the different hydrogel formulations. G) Atomic force microscopy (AFM) force–separation curves. H) Zn 2+ release profile. I) Degradation behavior of the hydrogel. J) Young's modulus of the different hydrogel formulations. Data are presented as mean ± SD. *p < 0.05 and ****p < 0.0001.
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Physicochemical and mechanical characterization of the hydrogel formulations. A) Schematic illustration of the preparation of GA–Zn 2+ /BP@SilMA and incorporation of osteogenic bone microtissues (BO). B) Photographs of the hydrogel precursor before and after ultraviolet. C) Scanning electron microscopy (SEM) images of SilMA, GA–Zn 2+ @SilMA, GA–Zn 2+ /BP@SilMA, and GA–Zn 2+ /BP/BO@SilMA hydrogels. D) <t>Energy-dispersive</t> <t>X-ray</t> spectroscopy (EDS) elemental maps of C, O, P, and Zn in the composite hydrogel. E) Fourier-transform infrared spectroscopy (FTIR) spectra of the different hydrogel formulations. F) X-ray <t>diffraction</t> (XRD) patterns of the different hydrogel formulations. G) Atomic force microscopy (AFM) force–separation curves. H) Zn 2+ release profile. I) Degradation behavior of the hydrogel. J) Young's modulus of the different hydrogel formulations. Data are presented as mean ± SD. *p < 0.05 and ****p < 0.0001.
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Physicochemical and mechanical characterization of the hydrogel formulations. A) Schematic illustration of the preparation of GA–Zn 2+ /BP@SilMA and incorporation of osteogenic bone microtissues (BO). B) Photographs of the hydrogel precursor before and after ultraviolet. C) Scanning electron microscopy (SEM) images of SilMA, GA–Zn 2+ @SilMA, GA–Zn 2+ /BP@SilMA, and GA–Zn 2+ /BP/BO@SilMA hydrogels. D) Energy-dispersive X-ray spectroscopy (EDS) elemental maps of C, O, P, and Zn in the composite hydrogel. E) Fourier-transform infrared spectroscopy (FTIR) spectra of the different hydrogel formulations. F) X-ray diffraction (XRD) patterns of the different hydrogel formulations. G) Atomic force microscopy (AFM) force–separation curves. H) Zn 2+ release profile. I) Degradation behavior of the hydrogel. J) Young's modulus of the different hydrogel formulations. Data are presented as mean ± SD. *p < 0.05 and ****p < 0.0001.

Journal: Materials Today Bio

Article Title: Hydrogel-integrated osteogenic microtissues promote repair of infected intervertebral defects through sequential immunomodulation

doi: 10.1016/j.mtbio.2026.103657

Figure Lengend Snippet: Physicochemical and mechanical characterization of the hydrogel formulations. A) Schematic illustration of the preparation of GA–Zn 2+ /BP@SilMA and incorporation of osteogenic bone microtissues (BO). B) Photographs of the hydrogel precursor before and after ultraviolet. C) Scanning electron microscopy (SEM) images of SilMA, GA–Zn 2+ @SilMA, GA–Zn 2+ /BP@SilMA, and GA–Zn 2+ /BP/BO@SilMA hydrogels. D) Energy-dispersive X-ray spectroscopy (EDS) elemental maps of C, O, P, and Zn in the composite hydrogel. E) Fourier-transform infrared spectroscopy (FTIR) spectra of the different hydrogel formulations. F) X-ray diffraction (XRD) patterns of the different hydrogel formulations. G) Atomic force microscopy (AFM) force–separation curves. H) Zn 2+ release profile. I) Degradation behavior of the hydrogel. J) Young's modulus of the different hydrogel formulations. Data are presented as mean ± SD. *p < 0.05 and ****p < 0.0001.

Article Snippet: Energy-dispersive X-ray spectroscopy (EDS, Sigma 360, ZEISS, Germany) mapping was used to examine the distribution of C, O, Zn, and P.Fourier transform infrared spectroscopy(FTIR, Nicolet iS50, Thermo Fisher Scientific, USA) was performed with 32 scans in the range of 4000–400 cm −1 at a resolution of 4 cm −1 ; X-ray diffraction patterns (XRD, SmartLab SE, Rigaku Corporation, Japan) were recorded from 5° to 90°.

Techniques: Scanning Electron Microscopy, Energy Dispersive X-Ray, Spectroscopy, Fourier Transform Infrared Spectroscopy, Microscopy