Journal: PLOS One

Article Title: Molecular changes, histopathology, and ultrasonic vocalization acoustic profiles of systemically dehydrated rats

doi: 10.1371/journal.pone.0322187

Figure Lengend Snippet: Schematic of the timeline used for the acclimation period (A1-A5), the behavioral conditioning period (B1-B5) and the experimental period (E1-E5). Information regarding the dehydration protocol, ultrasonic vocalization (USV) recording, and tissue collection is listed in each period. Image created with BioRender.com.

Article Snippet: The classification parameters were adapted from previously published protocols and the Metris USV categorization guidelines [ ].

Techniques:

Journal: Neurotrauma Reports

Article Title: Early-Life Mild Traumatic Brain Injury Alters Neurodevelopment and Behavior in Mice

doi: 10.1089/neur.2025.0016

Figure Lengend Snippet: Study design and timelines: (A) Post-natal day 7–8 pups were subjected to mild weight drop injuries using the Head Trauma Contusion Device for Rat and Mouse (RWD). (B) Mice were randomized to one of four groups including sham (no weight drop, n = 63), 0.01 J mTBI (10 g +10 cm drop, n = 26), 0.02 J mTBI (10 g + 20 cm drop or 20 g + 10 cm drop, n = 78), or 0.04 J mTBI (20 g + 20 cm drop, n = 38). Within the 0.02 J mTBI paradigm, both weight drop variables were controlled by equally randomizing mice to receive either double the weight ( n = 39) or double the height of weight drop ( n = 39) compared with the 0.01 J mTBI paradigm. (C) Weight gain (grams) from day of sham/TBI surgery to 5 dpi. Data are presented as mean ± standard deviation. dpi, days post injury; IHC, immunohistochemistry; J, joules; mTBI, mild traumatic brain injury; pnd, post-natal day; TBI, traumatic brain injury; USV, ultrasonic vocalization; WB, Western blotting.

Article Snippet: Isolation-induced USVs were recorded for 6 min to examine communication behavior post-injury using Avisoft Bioacoustics USV recording equipment and software (Avisoft-RECORDER, version 4.2) by removing the pup from their dam and placing each mouse individually in a sound-attenuating chamber (Med Associates Inc., ENV-022MD-27).

Techniques: Standard Deviation, Immunohistochemistry, Western Blot

Journal: Neurotrauma Reports

Article Title: Early-Life Mild Traumatic Brain Injury Alters Neurodevelopment and Behavior in Mice

doi: 10.1089/neur.2025.0016

Figure Lengend Snippet: Functional consequences of mTBI: Ultrasonic vocalizations. (A) Schematic depicting workflow for USV recordings: (1) Sham or mTBI pups were recorded for isolation-induced USV calls for 6 min, (2) USV waveforms were either accepted or rejected as background noise, and (3) typology call classification was performed, and call types were sorted as either “one-syllable” or “multisyllable.” (B) Total number of USV calls produced per group in males (N, sham: 15, 0.02 J mTBI: 13, 0.04 J mTBI: 10, outliers: 2 [1 in number total calls, 1 in latency analysis]). (C) Latency to produce the first USV call per group in males. (D) Total number of USV calls produced per group in females (N, sham: 10, 0.02 J mTBI: 12, 0.04 J mTBI: 14, outliers: 1 [in latency analysis]). (E) . Latency to produce the first USV call per group in females. Kruskal–Wallis test with Dunn’s post-hoc comparisons: * p < 0.05. (B–E) Male mTBI mice made similar number of overall calls and displayed similar latency to first call as sham males. However, female mTBI mice made less calls compared with sham females. (F) Percentage of “one-syllable” type calls and (G) “multisyllable” type calls out of total calls in males. (H) Percentage of “one-syllable” type and (I) “multisyllable” type calls out of total calls in females. One-way ANOVA with Tukey’s multiple comparisons test: # p < 0.1, * p < 0.05. Data are presented as mean ± standard deviation. (F–I) Male 0.04 J mTBI mice made less “one-syllable” and more “multisyllable” type calls compared with 0.02 J mTBI and sham male mice, whereas female mTBI mice did not differ in the types of calls produced compared with female sham mice. ANOVA, analysis of variance; dpi, days post injury; J, joules; mTBI, mild traumatic brain injury; pnd, post-natal day; USV, ultrasonic vocalization.

Article Snippet: Isolation-induced USVs were recorded for 6 min to examine communication behavior post-injury using Avisoft Bioacoustics USV recording equipment and software (Avisoft-RECORDER, version 4.2) by removing the pup from their dam and placing each mouse individually in a sound-attenuating chamber (Med Associates Inc., ENV-022MD-27).

Techniques: Functional Assay, Isolation, Produced, Standard Deviation