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Characterization of sEVs and PKH67‐labelled sEVs uptake. The size distribution of sEVs by NTA from the (A) static (collected at D27‐30), (B) orbital shaker (collected at D9–12) and (C) Organoid‐on‐a‐Chip (collected at D27–30) were determined. (D–F) sEVs STEM images from (D) static (at D18–21), (E) orbital shaker (D27–30), (F) Organoid‐on‐a‐Chip (D9–12). (G) The enrichment of sEVs marker Alix and the low abundance of non‐sEV marker Calnexin were detected with Western blotting. (H, I) Uptake of PKH67‐labelled sEVs by <t>U87</t> <t>Glioblastoma</t> cells was demonstrated (scale bar 200 µm). Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01 compared to the control group.
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Characterization of sEVs and PKH67‐labelled sEVs uptake. The size distribution of sEVs by NTA from the (A) static (collected at D27‐30), (B) orbital shaker (collected at D9–12) and (C) Organoid‐on‐a‐Chip (collected at D27–30) were determined. (D–F) sEVs STEM images from (D) static (at D18–21), (E) orbital shaker (D27–30), (F) Organoid‐on‐a‐Chip (D9–12). (G) The enrichment of sEVs marker Alix and the low abundance of non‐sEV marker Calnexin were detected with Western blotting. (H, I) Uptake of PKH67‐labelled sEVs by <t>U87</t> <t>Glioblastoma</t> cells was demonstrated (scale bar 200 µm). Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01 compared to the control group.
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Characterization of sEVs and PKH67‐labelled sEVs uptake. The size distribution of sEVs by NTA from the (A) static (collected at D27‐30), (B) orbital shaker (collected at D9–12) and (C) Organoid‐on‐a‐Chip (collected at D27–30) were determined. (D–F) sEVs STEM images from (D) static (at D18–21), (E) orbital shaker (D27–30), (F) Organoid‐on‐a‐Chip (D9–12). (G) The enrichment of sEVs marker Alix and the low abundance of non‐sEV marker Calnexin were detected with Western blotting. (H, I) Uptake of PKH67‐labelled sEVs by <t>U87</t> <t>Glioblastoma</t> cells was demonstrated (scale bar 200 µm). Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01 compared to the control group.
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Characterization of sEVs and PKH67‐labelled sEVs uptake. The size distribution of sEVs by NTA from the (A) static (collected at D27‐30), (B) orbital shaker (collected at D9–12) and (C) Organoid‐on‐a‐Chip (collected at D27–30) were determined. (D–F) sEVs STEM images from (D) static (at D18–21), (E) orbital shaker (D27–30), (F) Organoid‐on‐a‐Chip (D9–12). (G) The enrichment of sEVs marker Alix and the low abundance of non‐sEV marker Calnexin were detected with Western blotting. (H, I) Uptake of PKH67‐labelled sEVs by <t>U87</t> <t>Glioblastoma</t> cells was demonstrated (scale bar 200 µm). Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01 compared to the control group.
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Characterization of sEVs and PKH67‐labelled sEVs uptake. The size distribution of sEVs by NTA from the (A) static (collected at D27‐30), (B) orbital shaker (collected at D9–12) and (C) Organoid‐on‐a‐Chip (collected at D27–30) were determined. (D–F) sEVs STEM images from (D) static (at D18–21), (E) orbital shaker (D27–30), (F) Organoid‐on‐a‐Chip (D9–12). (G) The enrichment of sEVs marker Alix and the low abundance of non‐sEV marker Calnexin were detected with Western blotting. (H, I) Uptake of PKH67‐labelled sEVs by U87 Glioblastoma cells was demonstrated (scale bar 200 µm). Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01 compared to the control group.

Journal: Developmental Neurobiology

Article Title: Molecular and Proteomic Profiling of Human Cerebral Organoid‑Derived Small Extracellular Vesicles

doi: 10.1002/dneu.70062

Figure Lengend Snippet: Characterization of sEVs and PKH67‐labelled sEVs uptake. The size distribution of sEVs by NTA from the (A) static (collected at D27‐30), (B) orbital shaker (collected at D9–12) and (C) Organoid‐on‐a‐Chip (collected at D27–30) were determined. (D–F) sEVs STEM images from (D) static (at D18–21), (E) orbital shaker (D27–30), (F) Organoid‐on‐a‐Chip (D9–12). (G) The enrichment of sEVs marker Alix and the low abundance of non‐sEV marker Calnexin were detected with Western blotting. (H, I) Uptake of PKH67‐labelled sEVs by U87 Glioblastoma cells was demonstrated (scale bar 200 µm). Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01 compared to the control group.

Article Snippet: Human U87 glioblastoma cells (American Type Culture Collection) were cultured in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin.

Techniques: Marker, Western Blot, Control

Effects sEVs obtained from static, orbital shaker and Organoid‐on‐a‐Chip conditions on U87 glioblastoma cells. (A) MTT assay for viability of U87 Glioblastoma for static, orbital shaker, and Organoid‐on‐a‐Chip sEVs. (B, C) Representative Ki67/DAPI immunofluorescence images of U87 glioblastoma cells following sEV treatment and quantification of Ki67–DAPI signal overlap using the Manders coefficient. (D, E) Migration of U87 Glioblastoma cells upon sEV treatment was observed by scratch assay up to 48 h. Scale bar 400 µm. (F, G) Diameter of spheres formed on day 2 and day 4 of sEVs treated U87 Glioblastoma by hanging drop sphere formation assay were quantified. Scale bar 200 µm. Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01 compared to the control group.

Journal: Developmental Neurobiology

Article Title: Molecular and Proteomic Profiling of Human Cerebral Organoid‑Derived Small Extracellular Vesicles

doi: 10.1002/dneu.70062

Figure Lengend Snippet: Effects sEVs obtained from static, orbital shaker and Organoid‐on‐a‐Chip conditions on U87 glioblastoma cells. (A) MTT assay for viability of U87 Glioblastoma for static, orbital shaker, and Organoid‐on‐a‐Chip sEVs. (B, C) Representative Ki67/DAPI immunofluorescence images of U87 glioblastoma cells following sEV treatment and quantification of Ki67–DAPI signal overlap using the Manders coefficient. (D, E) Migration of U87 Glioblastoma cells upon sEV treatment was observed by scratch assay up to 48 h. Scale bar 400 µm. (F, G) Diameter of spheres formed on day 2 and day 4 of sEVs treated U87 Glioblastoma by hanging drop sphere formation assay were quantified. Scale bar 200 µm. Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01 compared to the control group.

Article Snippet: Human U87 glioblastoma cells (American Type Culture Collection) were cultured in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin.

Techniques: MTT Assay, Immunofluorescence, Migration, Scratch Assay, Tube Formation Assay, Control