Journal: Frontiers in Oncology

Article Title: ZNF25 as an immunotherapy target: pan-cancer biomarker potential and mechanistic exploration in glioma

doi: 10.3389/fonc.2026.1631383

Figure Lengend Snippet: ZNF25 knockdown inhibits proliferation and migration of A172 and U87-MG Cells. (A) The silencing efficiency of siRNAs for ZNF25 knockdown in A172 cells detected by RT-qPCR. (B) The silencing efficiency of siRNAs for ZNF25 knockdown in U87-MG cells detected by RT-qPCR. (C) ZNF25 protein levels in A172 cells were analyzed by Western blotting at 72 hours post-transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (D) ZNF25 protein levels in U87-MG cells were analyzed by Western blotting at 72 hours post-transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (E) Bar graph showing the quantification of protein expression in A172 cells. The band intensities from Western blot analysis were quantified using ImageJ software. Data are presented as mean ± SD (n=3 independent experiment, Student’s t-test). (F) Bar graph showing the quantification of protein expression in U87-MG cells. The band intensities from Western blot analysis were quantified using ImageJ software. Data are presented as mean ± SD (n=3 independent experiment, Student’s t-test). (G–H) Cell viability was determined by CCK-8 assay in A172 and U87-MG cells after transfection with si ZNF25 and siNC. Data are presented as the mean ± SD of at least three independent experiments compared with the siNC group at the corresponding time point. (I) Representative microscopic images of the wound area at 36h after scratching in A172 cells transfected with si ZNF25 and siNC. Scale bar, 400 μm. (J) Quantitative analysis of the wound closure rate of A172 cells at 36h post-transfection with si ZNF25 and siNC. (K) Representative microscopic images of the wound area at 36h after scratching in U87-MG cells transfected with si ZNF25 and siNC. Scale bar, 400μm. (L) Quantitative analysis of wound closure rate in U87-MG cells at 36h post-transfection, with si ZNF25 and siNC. Data are shown as mean ± SD from n = 6 independent experiments. Statistical significance was determined by Student’s t-test. (* P <0.05, ** P< 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: Glioma cells U87-MG were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and A172 were purchased from Xiamen Immocell Biotechnology Co. Ltd.

Techniques: Knockdown, Migration, Quantitative RT-PCR, Western Blot, Transfection, Control, Expressing, Software, CCK-8 Assay

Journal: Frontiers in Oncology

Article Title: ZNF25 as an immunotherapy target: pan-cancer biomarker potential and mechanistic exploration in glioma

doi: 10.3389/fonc.2026.1631383

Figure Lengend Snippet: Functional enrichment analysis and molecular verification of ZNF25 knockdown. (A) The volcano plot visualized all identified DEGs in U87-MG cells, which were screened by the criteria of adjusted P-value (padj) < 0.05 and ∣log 2 FC∣>1. (B) Top 10 enriched GO terms across all categories. (C) Top 10 enriched KEGG pathways. (D) Western blot analysis of p-AKT protein levels in A172 and U87-MG cells at 72h after transfection with si ZNF25 and siNC. GAPDH was used as a loading control. (E) Bar graph showing the quantification of protein expression in U87-MG cells corresponding to (D) . Data are presented as mean ± SD (n = 3 independent experiment, Student’s t-test). (* P < 0.05, ** P < 0.01).

Article Snippet: Glioma cells U87-MG were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and A172 were purchased from Xiamen Immocell Biotechnology Co. Ltd.

Techniques: Functional Assay, Knockdown, Western Blot, Transfection, Control, Expressing