Review





Similar Products

triptolide  (Enamine Ltd)
99
Enamine Ltd triptolide
Triptolide, supplied by Enamine Ltd, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/triptolide/Triptolide/custom%40en300-19745751%4042515771
Average 99 stars, based on 1 article reviews
triptolide - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
1000x stock concentration  (MedChemExpress)
95
MedChemExpress 1000x stock concentration
1000x Stock Concentration, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/triptolide/Triptolide/bio_rxiv__64898__2026__05__24__727549-348-3-9
Average 95 stars, based on 1 article reviews
1000x stock concentration - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
triptolide  (Thermo Fisher)
94
Thermo Fisher triptolide
Causes of replication fork processivity differences across the genome. ( A ) Metaplot of TrAEL-seq read count in DLD-1 cells averaged across genes ± 100 kb. Genes are stratified for transcriptional activity based on PRO-seq into 0%–40%, 40–70%, 70%–90%, and 90%–100% categories. Profiles were normalized individually to make background read counts as close as possible. ( B ) Plots of total TrAEL-seq read count at increasing distance from replication Initiation Zones, stratified for nascent transcription level by PRO-seq. Analysis was performed as in Fig. , but the genomic windows included were filtered to remove the top 25%, 50%, or 75% of regions based on PRO-seq read count. ( C ) Total TrAEL-seq read count at increasing distance from replication Initiation Zones in untreated DLD-1 cells or cells treated for 2 h with 100 µM DRB or for 4 h with 3 µM <t>triptolide.</t> ( D ) Metaplot of TrAEL-seq read count over genes ± 100 kb as in panel (A) in DLD-1 cells ± DRB and <t>triptolide</t> (datasets as in panel (C), data is an average of the two biological replicates shown). ( E ) Total TrAEL-seq read count at increasing distance from replication Initiation Zones in untreated DLD-1 cells and cells treated for with 1 µM Cerelasertib for 24 h. ( F ) Total TrAEL-seq read count at increasing distance from replication Initiation Zones in untreated PC9 cells and cells treated for 24 h with 16 nM palbociclib.
Triptolide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/triptolide/Triptolide/pmc12988324-38-32-33
Average 94 stars, based on 1 article reviews
triptolide - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
triptolide  (MedChemExpress)
95
MedChemExpress triptolide
Causes of replication fork processivity differences across the genome. ( A ) Metaplot of TrAEL-seq read count in DLD-1 cells averaged across genes ± 100 kb. Genes are stratified for transcriptional activity based on PRO-seq into 0%–40%, 40–70%, 70%–90%, and 90%–100% categories. Profiles were normalized individually to make background read counts as close as possible. ( B ) Plots of total TrAEL-seq read count at increasing distance from replication Initiation Zones, stratified for nascent transcription level by PRO-seq. Analysis was performed as in Fig. , but the genomic windows included were filtered to remove the top 25%, 50%, or 75% of regions based on PRO-seq read count. ( C ) Total TrAEL-seq read count at increasing distance from replication Initiation Zones in untreated DLD-1 cells or cells treated for 2 h with 100 µM DRB or for 4 h with 3 µM <t>triptolide.</t> ( D ) Metaplot of TrAEL-seq read count over genes ± 100 kb as in panel (A) in DLD-1 cells ± DRB and <t>triptolide</t> (datasets as in panel (C), data is an average of the two biological replicates shown). ( E ) Total TrAEL-seq read count at increasing distance from replication Initiation Zones in untreated DLD-1 cells and cells treated for with 1 µM Cerelasertib for 24 h. ( F ) Total TrAEL-seq read count at increasing distance from replication Initiation Zones in untreated PC9 cells and cells treated for 24 h with 16 nM palbociclib.
Triptolide, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/triptolide/Triptolide/10__1016_slash_j__clnves__2026__100076-143-16-20
Average 95 stars, based on 1 article reviews
triptolide - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
triptolide  (Baoji Titanium Industry Co Ltd)
86
Baoji Titanium Industry Co Ltd triptolide
Causes of replication fork processivity differences across the genome. ( A ) Metaplot of TrAEL-seq read count in DLD-1 cells averaged across genes ± 100 kb. Genes are stratified for transcriptional activity based on PRO-seq into 0%–40%, 40–70%, 70%–90%, and 90%–100% categories. Profiles were normalized individually to make background read counts as close as possible. ( B ) Plots of total TrAEL-seq read count at increasing distance from replication Initiation Zones, stratified for nascent transcription level by PRO-seq. Analysis was performed as in Fig. , but the genomic windows included were filtered to remove the top 25%, 50%, or 75% of regions based on PRO-seq read count. ( C ) Total TrAEL-seq read count at increasing distance from replication Initiation Zones in untreated DLD-1 cells or cells treated for 2 h with 100 µM DRB or for 4 h with 3 µM <t>triptolide.</t> ( D ) Metaplot of TrAEL-seq read count over genes ± 100 kb as in panel (A) in DLD-1 cells ± DRB and <t>triptolide</t> (datasets as in panel (C), data is an average of the two biological replicates shown). ( E ) Total TrAEL-seq read count at increasing distance from replication Initiation Zones in untreated DLD-1 cells and cells treated for with 1 µM Cerelasertib for 24 h. ( F ) Total TrAEL-seq read count at increasing distance from replication Initiation Zones in untreated PC9 cells and cells treated for 24 h with 16 nM palbociclib.
Triptolide, supplied by Baoji Titanium Industry Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/triptolide/triptolide/10__2147_slash_ijn__s567165-48-0-7
Average 86 stars, based on 1 article reviews
triptolide - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier

Image Search Results


Causes of replication fork processivity differences across the genome. ( A ) Metaplot of TrAEL-seq read count in DLD-1 cells averaged across genes ± 100 kb. Genes are stratified for transcriptional activity based on PRO-seq into 0%–40%, 40–70%, 70%–90%, and 90%–100% categories. Profiles were normalized individually to make background read counts as close as possible. ( B ) Plots of total TrAEL-seq read count at increasing distance from replication Initiation Zones, stratified for nascent transcription level by PRO-seq. Analysis was performed as in Fig. , but the genomic windows included were filtered to remove the top 25%, 50%, or 75% of regions based on PRO-seq read count. ( C ) Total TrAEL-seq read count at increasing distance from replication Initiation Zones in untreated DLD-1 cells or cells treated for 2 h with 100 µM DRB or for 4 h with 3 µM triptolide. ( D ) Metaplot of TrAEL-seq read count over genes ± 100 kb as in panel (A) in DLD-1 cells ± DRB and triptolide (datasets as in panel (C), data is an average of the two biological replicates shown). ( E ) Total TrAEL-seq read count at increasing distance from replication Initiation Zones in untreated DLD-1 cells and cells treated for with 1 µM Cerelasertib for 24 h. ( F ) Total TrAEL-seq read count at increasing distance from replication Initiation Zones in untreated PC9 cells and cells treated for 24 h with 16 nM palbociclib.

Journal: Nucleic Acids Research

Article Title: Multiplexed TrAEL-seq captures DNA replication dynamics in mammalian cells

doi: 10.1093/nar/gkag212

Figure Lengend Snippet: Causes of replication fork processivity differences across the genome. ( A ) Metaplot of TrAEL-seq read count in DLD-1 cells averaged across genes ± 100 kb. Genes are stratified for transcriptional activity based on PRO-seq into 0%–40%, 40–70%, 70%–90%, and 90%–100% categories. Profiles were normalized individually to make background read counts as close as possible. ( B ) Plots of total TrAEL-seq read count at increasing distance from replication Initiation Zones, stratified for nascent transcription level by PRO-seq. Analysis was performed as in Fig. , but the genomic windows included were filtered to remove the top 25%, 50%, or 75% of regions based on PRO-seq read count. ( C ) Total TrAEL-seq read count at increasing distance from replication Initiation Zones in untreated DLD-1 cells or cells treated for 2 h with 100 µM DRB or for 4 h with 3 µM triptolide. ( D ) Metaplot of TrAEL-seq read count over genes ± 100 kb as in panel (A) in DLD-1 cells ± DRB and triptolide (datasets as in panel (C), data is an average of the two biological replicates shown). ( E ) Total TrAEL-seq read count at increasing distance from replication Initiation Zones in untreated DLD-1 cells and cells treated for with 1 µM Cerelasertib for 24 h. ( F ) Total TrAEL-seq read count at increasing distance from replication Initiation Zones in untreated PC9 cells and cells treated for 24 h with 16 nM palbociclib.

Article Snippet: Hydroxyurea (Merck H78627 ) was used at 20–100 μM for 2 h, Ceralasertib (Merck TA9H11E41972) at 1 μM for 24 h, palbociclib (Thermo Fisher Scientific 16430568) at 16 nM for 24 h, triptolide (Thermo Fisher Scientific PG490) at 3 μM for 4 h.

Techniques: Activity Assay