Journal: Frontiers in Oncology

Article Title: PFKFB3 Regulates Chemoresistance, Metastasis and Stemness via IAP Proteins and the NF-κB Signaling Pathway in Ovarian Cancer

doi: 10.3389/fonc.2022.748403

Figure Lengend Snippet: Overexpression of PFKFB3 in ovarian cancer is correlated with metastasis and poor prognosis. (A) Representative images of PFKFB3 expression in (i) normal ovarian tissue, (ii) serous, (iii) mucinous, (iv) endometrioid and (v) clear cell subtypes of tumor samples on ovarian cancer TMA (OVC1021, Biomax) by IHC staining (scale bar, 100 μm). The insets highlight regions with higher magnification. (B) Association of PFKFB3 with stage/grade/histotype in ovarian cancer. The graphs for stage and grade encompass all four histotypes. (C) Representative images of IHC staining for PFKFB3 expression in ovarian primary tumors (i, iii) and their metastatic foci (ii, iv; scale bar, 100 μm). (D) Bar chart showing PFKFB3 staining in 13 pairs of tissues. (E) Relative PFKFB3 mRNA expression in HOSE 11-12, primary cancer cells and matched ascites cancer cells from high-grade serous ovarian cancer, determined by qPCR. (F) PFKFB3 expression in HOSE 11-12 and ovarian cancer cell lines (SKOV3, OVCAR3, and TOV112D) at the mRNA level, determined by qPCR. (G) (left) Overall and (right) progression-free survival rates were analyzed with the log-rank test for PFKFB3 high/low groups of ovarian cancer patients obtained from GSE26193 (n = 107), by using the Kaplan-Meier plotter. (*p < 0.05, **p < 0.01; n.s., not significant).

Article Snippet: A tissue microarray analysis (TMA, OVC1021; Biomax) with 102 cores from four normal benign and 97 ovarian cancer tissues was performed in duplicate.

Techniques: Over Expression, Expressing, Immunohistochemistry, Staining

Journal: American Journal of Clinical and Experimental Urology

Article Title: Predictive value of pseudouridine in prostate cancer

doi:

Figure Lengend Snippet: A. Representative images (10× with 40× insert) of normal adjacent tissues (NAT) and adenocarcinomas. A tissue micro array (TMA) purchased from US Biomax, Inc. (PR242b) was stained for pseudouridine and imaged at 10X and 40X. Cores with Gleason 3+3, 4+3 and 5+4 were used for representative images. Staining is primarily localized to the cytoplasm of glandular cells with negligible staining of the gland cells belonging to normal adjacent tissues (NATs). B. Plot of H-scores of normal adjacent tissues and adenocarcinomas. Each individual core was evaluated by an in-house pathologist for the presence of cancer, and given an H-score. The final H-score is the summation of (1+i)pi where i is the intensity score [0 (lowest)1+, 2+, 3+, 4+(highest)] and pi is the percent of the cells with that intensity. The p-value was calculated using a two-tailed unpaired t-test in Prism, where an alpha value of 0.05 was considered significant.

Article Snippet: TMA immunohistochemistry and analysis Human prostate cancer tissue microarray (TMA) PR242b was purchased from US Biomax, Inc. (Derwood, MD, USA).

Techniques: Microarray, Staining, Two Tailed Test

Journal: Toxicology and applied pharmacology

Article Title: Inhibition of nicotinamide phosphoribosyltransferase and depletion of nicotinamide adenine dinucleotide contribute to arsenic trioxide suppression of oral squamous cell carcinoma

doi: 10.1016/j.taap.2017.05.008

Figure Lengend Snippet: NAMPT expression was analyzed with tissue microarray (TMA). Positive immunostaining for NAMPT was brown and indicated by arrow. (A) Representative immunohistochemical staining of NAMPT expression. (B) Relative intensity fold change of NAMPT expression in normal and adjacent tissue (NAT) and OSCC tissues of three grades of differentiation. **p<0.01 vs. NAT; #p<0.05 vs. well differentiated OSCC; !p<0.05 vs. moderately differentiated OSCC. Bar=100μm.

Article Snippet: The specimens of OSCC for tissue microarray (TMA) analysis were purchased from Biomax US (Rockville, MD), catalog numbers OR601a.

Techniques: Expressing, Microarray, Immunostaining, Immunohistochemical staining, Staining

Journal: Scientific Reports

Article Title: Yes-Associated Protein (YAP) Promotes Tumorigenesis in Melanoma Cells Through Stimulation of Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1)

doi: 10.1038/s41598-017-14764-4

Figure Lengend Snippet: The consistency of YAP and LRP1 in tissue microarray specimen. ( a , b ) TMA slides include forty skin melanoma tissues and eight skin normal tissues which locate on the bottom of the each TMA. Representative images of IHC from HCC TMA stained with anti-YAP or anti-LRP1 antibodies. Scale bar, 100 μM. ( c ) Representative images of IHC from skin melanoma HCC TMA stained with anti-YAP or anti-LRP1 antibodies. Scale bar, 100 μM. ( d ) The statistical figure of skin melanoma IHC images from HCC TMA stained with anti-YAP or anti-LRP1 antibodies. The TMA data were analyzed using the χ2 test.

Article Snippet: For IHC, melanoma tissue microarray analysis (TMA) slides were purchased from Biomax (Rockville, MD, USA).

Techniques: Microarray, Staining