Journal: Stem Cells Translational Medicine

Article Title: Extracellular vesicles conjugated with c(RGDyk) peptide targeting integrin αVβ3 repair optic nerve injury through YAP/TAZ and Smad2/3 signaling

doi: 10.1093/stcltm/szag006

Figure Lengend Snippet: Validation of target genes obtained via single-cell RNA sequencing in vivo/vitro models. Levels of interested proteins in (A) the retina and (B) optic nerve of ONC were presented. During recovery process by SC_EVs, protein levels involved in signal pathway were confirmed in (C) retina of ONC models and (D) R28 cells (* P < .05, ** P < .05 vs the age-matched sham; # P < .05, ## P < .01 vs EVs). At 9 h prior to SC_EVs treatment, R28 cells were exposed to 200 µM of CoCl 2 and 1 µM of TM2 TEAD inhibitor. Then, by (D) immunoblot and (E) immunofluorescence were performed for expression of proteins. The results are expressed as the mean ± standard error of the mean (SEM; scale bar: 10 μm). Statistical significance was determined using a nonparametric statistical test, followed by the Mann–Whitney U test (* P < .05, ** P < .01 vs control; # P < .05, ## P < .01vs CoCl 2 ; ++ P < .01 vs CoCl 2 +SC_EVs).

Article Snippet: Aliquots of 2 × 10 5 R28 cells were seeded onto glass coverslips and treated with 200 μM CoCl 2 and 1 μM of the TEA/TEF-domain (TEAD) transcription factor inhibitor TM2 (Tocris Bioscience, Bristol, UK) for 9 h. The hypoxia-damaged R28 cells were then incubated with EVs at a concentration of 24 μg/mL.

Techniques: Biomarker Discovery, Single Cell, RNA Sequencing, In Vivo, Western Blot, Immunofluorescence, Expressing, MANN-WHITNEY, Control