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Journal: iScience

Article Title: Small-molecule activators of the Staphylococcus aureus ClpC/ClpP AAA+ protease

doi: 10.1016/j.isci.2026.117738

Figure Lengend Snippet:

Article Snippet: After cell lysis using a French press, the cell debris was removed by centrifugation at 18,000 g for 60 min at 4°C, and the cleared lysate was subsequently transferred into a plastic column containing pre-equilibrated 0.8–1 g Protino Ni-IDA resin (Macherey-Nagel) at 4°C.

Techniques: Virus, Recombinant, Protease Inhibitor, Staining, Cloning, Mutagenesis, Sequencing, Software, Analysis, Microscopy