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High-performance thin-layer chromatography (HPTLC) analysis of South African propolis extract. The representative plate was visualized under (A) UV 366 nm, (B) white light reflectance, and (C) a bioautogram showing the antibacterial activity of selected bands against S. <t>pneumoniae</t> . (D) a DESI-MSI overlay of all bands present within the antibacterial region. (E) 1 – (E) 9 DESI–MS images of selected chromatographic bands, identified as: (E) 1 , Pinobanksin; (E) 2 , Chrysin; (E) 3 , Galangin; (E) 4 , Pinobanksin acetate; (E) 5 , Pinobanksin propionate; (E) 6 , Pinocembrin; (E) 7 , Pinobanksin butyrate; (E) 8 , Pinobanksin pentanoate; and E 9 , Pinobanksin hexanoate. Ion intensity signals were normalized to the total ion current and displayed as a heat map. Scale bar = 11.55 mm.
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High-performance thin-layer chromatography (HPTLC) analysis of South African propolis extract. The representative plate was visualized under (A) UV 366 nm, (B) white light reflectance, and (C) a bioautogram showing the antibacterial activity of selected bands against S. <t>pneumoniae</t> . (D) a DESI-MSI overlay of all bands present within the antibacterial region. (E) 1 – (E) 9 DESI–MS images of selected chromatographic bands, identified as: (E) 1 , Pinobanksin; (E) 2 , Chrysin; (E) 3 , Galangin; (E) 4 , Pinobanksin acetate; (E) 5 , Pinobanksin propionate; (E) 6 , Pinocembrin; (E) 7 , Pinobanksin butyrate; (E) 8 , Pinobanksin pentanoate; and E 9 , Pinobanksin hexanoate. Ion intensity signals were normalized to the total ion current and displayed as a heat map. Scale bar = 11.55 mm.
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High-performance thin-layer chromatography (HPTLC) analysis of South African propolis extract. The representative plate was visualized under (A) UV 366 nm, (B) white light reflectance, and (C) a bioautogram showing the antibacterial activity of selected bands against S. pneumoniae . (D) a DESI-MSI overlay of all bands present within the antibacterial region. (E) 1 – (E) 9 DESI–MS images of selected chromatographic bands, identified as: (E) 1 , Pinobanksin; (E) 2 , Chrysin; (E) 3 , Galangin; (E) 4 , Pinobanksin acetate; (E) 5 , Pinobanksin propionate; (E) 6 , Pinocembrin; (E) 7 , Pinobanksin butyrate; (E) 8 , Pinobanksin pentanoate; and E 9 , Pinobanksin hexanoate. Ion intensity signals were normalized to the total ion current and displayed as a heat map. Scale bar = 11.55 mm.

Journal: Frontiers in Plant Science

Article Title: DESI-MSI applications for direct (spatial) biomolecular analysis of South African natural medicinal products

doi: 10.3389/fpls.2026.1802351

Figure Lengend Snippet: High-performance thin-layer chromatography (HPTLC) analysis of South African propolis extract. The representative plate was visualized under (A) UV 366 nm, (B) white light reflectance, and (C) a bioautogram showing the antibacterial activity of selected bands against S. pneumoniae . (D) a DESI-MSI overlay of all bands present within the antibacterial region. (E) 1 – (E) 9 DESI–MS images of selected chromatographic bands, identified as: (E) 1 , Pinobanksin; (E) 2 , Chrysin; (E) 3 , Galangin; (E) 4 , Pinobanksin acetate; (E) 5 , Pinobanksin propionate; (E) 6 , Pinocembrin; (E) 7 , Pinobanksin butyrate; (E) 8 , Pinobanksin pentanoate; and E 9 , Pinobanksin hexanoate. Ion intensity signals were normalized to the total ion current and displayed as a heat map. Scale bar = 11.55 mm.

Article Snippet: Haemophilus Test Medium (HTM), HTM Supplement SR0158, Streptococcus pneumoniae (ATCC 6841) was sourced from University of Witwatersrand (Johannesburg, South Africa), super pure-grade methanol was obtained from Romil (Johannesburg, South Africa).

Techniques: High Performance Thin Layer Chromatography, Activity Assay