Journal: Alzheimer's & Dementia

Article Title: ApoE lipidation state directs immunometabolic reprogramming of human microglia

doi: 10.1002/alz.71882

Figure Lengend Snippet: Lipid‐free ApoE4 polarizes microglia to interferon and pattern recognition signaling and away from proteostasis programs. (A) PLS‐DA plot of LF‐E4 versus rHDL‐E4 treatment at 24‐h time point. (B) VIP score table displaying top 25 proteins driving differences in PLS‐DA plot. (C) Volcano plot displaying LF‐E4 versus rHDL‐E4, with x ‐axis representing log 2 FC and y ‐axis representing −log 10 ( p value). Purple indicates downregulated proteins in response to LF‐E4 treatment, while orange indicates upregulated proteins in response to LF‐E4 treatment, at 24 h. (D) Heatmap of top 50 DAP in 24‐h group. The color gradient is associated with the normalized concentration. (E) Upregulated biological pathway analysis of DAPs in response to LF‐E4 versus rHDL‐E4 treatment upregulated in LF‐E4) at 24 h. (F) Upregulated KEGG pathway analysis of DAPs in response to LF‐E4 versus rHDL‐E4 treatment (upregulated in LF‐E4) at 24 h. (G) Relative gene expression of STAT1 and SP100. (H) Venn diagram of overlapping DAPs between LF‐E3 versus rHDL‐E3 treatment compared to LF‐E4 versus rHDL‐E4 treatment. (I) Heatmap of proteins of interest. Columns represent comparisons between groups at 6‐ and 24‐h time points. Rows indicate proteins categorized by function. Color scale indicates fold enrichment representing left versus right group's downregulation shown by a purple gradient and upregulation by an orange gradient. Data are mean ± SEM, n = 3, **p < 0.001, ****p < 0.0001.

Article Snippet: A subset of cells was treated with the STAT1 inhibitor Fludarabine (MedChemExpress, Catalog No.: HY‐B0069) at the same time as LF‐ApoE4 (LF‐E4).

Techniques: Concentration Assay, Analysis, Gene Expression

Journal: Frontiers in Physiology

Article Title: Kaempferol ameliorates gestational diabetes mellitus by regulating STAT3-associated AIM2 inflammasome activation and ferroptotic injury in trophoblasts

doi: 10.3389/fphys.2026.1857741

Figure Lengend Snippet: STAT3-associated changes in STAT1/IRF1 signaling and AIM2 expression in HG-stimulated trophoblast cells. (A, B) RT-qPCR was performed to assess STAT3 knockdown efficiency and AIM2 mRNA expression changes in HTR-8/SVneo cells subjected to different treatments. (C–F) Western blot analysis was performed to evaluate the expression levels of p-STAT1, STAT1, IRF1, and AIM2 proteins under different experimental conditions. N = 3, not significant, ** P <0.01, *** P <0.001.

Article Snippet: Following blocking, the membranes were incubated overnight at 4°C with primary antibodies, including AIM2 (1:1000, catalog number ab93015, Abcam), Glutathione Peroxidase 4 (GPX4, 1:1000, 52455, Cell Signaling Technology, CST), solute carrier family 7 member 11 (SLC7A11, 1:1000, 23276, CST), phospho-STAT3 (p-STAT3, 1:1000, 9145, CST), STAT3 (1:1000, 12640, CST), pro-IL-1β (1:1000, 12703, CST), IL-1β (1:1000, ab283818, Abcam), cleaved caspase-1 (1:1000, 4199, CST), pro-caspase-1 (1:1000, 24232, CST), STAT1 (1:1000, 9172T, CST), phospho-STAT1 (p-STAT1, 1:1000, 9177S, CST), IRF1 (1:1000, 9172T, CST) and β-actin (1:1000, 4967S, CST).

Techniques: Expressing, Quantitative RT-PCR, Knockdown, Western Blot, Analysis

Journal: Frontiers in Physiology

Article Title: Kaempferol ameliorates gestational diabetes mellitus by regulating STAT3-associated AIM2 inflammasome activation and ferroptotic injury in trophoblasts

doi: 10.3389/fphys.2026.1857741

Figure Lengend Snippet: STAT3-associated changes in STAT1/IRF1 signaling and AIM2 expression in HG-stimulated trophoblast cells. (A, B) RT-qPCR was performed to assess STAT3 knockdown efficiency and AIM2 mRNA expression changes in HTR-8/SVneo cells subjected to different treatments. (C–F) Western blot analysis was performed to evaluate the expression levels of p-STAT1, STAT1, IRF1, and AIM2 proteins under different experimental conditions. N = 3, not significant, ** P <0.01, *** P <0.001.

Article Snippet: Following blocking, the membranes were incubated overnight at 4°C with primary antibodies, including AIM2 (1:1000, catalog number ab93015, Abcam), Glutathione Peroxidase 4 (GPX4, 1:1000, 52455, Cell Signaling Technology, CST), solute carrier family 7 member 11 (SLC7A11, 1:1000, 23276, CST), phospho-STAT3 (p-STAT3, 1:1000, 9145, CST), STAT3 (1:1000, 12640, CST), pro-IL-1β (1:1000, 12703, CST), IL-1β (1:1000, ab283818, Abcam), cleaved caspase-1 (1:1000, 4199, CST), pro-caspase-1 (1:1000, 24232, CST), STAT1 (1:1000, 9172T, CST), phospho-STAT1 (p-STAT1, 1:1000, 9177S, CST), IRF1 (1:1000, 9172T, CST) and β-actin (1:1000, 4967S, CST).

Techniques: Expressing, Quantitative RT-PCR, Knockdown, Western Blot, Analysis