Journal: Annals of Medicine

Article Title: Association between anti-nephrin autoantibodies and disease activity in minimal change disease

doi: 10.1080/07853890.2026.2661115

Figure Lengend Snippet: Assay to detect nephrin autoantibodies.

Article Snippet: Biotinylated recombinant human nephrin detection protein (17757-H08H, Sino Biological), diluted 1:20,000, was added to the Protein G plates (100 μL/well) and incubated at 37 °C with shaking at 700 rpm for 60 ± 5 min, followed by washing three times with PBST.

Techniques:

Journal: Annals of Medicine

Article Title: Association between anti-nephrin autoantibodies and disease activity in minimal change disease

doi: 10.1080/07853890.2026.2661115

Figure Lengend Snippet: Detection of serum anti-nephrin autoantibodies by ELISA. (a) Serum levels of anti-nephrin autoantibodies in patients with minimal change disease (MCD), primary focal segmental glomerulosclerosis (pFSGS), membranous nephropathy (MN), lupus nephritis (LN), diabetic nephropathy (DN), IgA nephropathy (IgAN), and healthy controls. (b) Positive rates of anti-nephrin autoantibodies in the same cohorts.

Article Snippet: Biotinylated recombinant human nephrin detection protein (17757-H08H, Sino Biological), diluted 1:20,000, was added to the Protein G plates (100 μL/well) and incubated at 37 °C with shaking at 700 rpm for 60 ± 5 min, followed by washing three times with PBST.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Annals of Medicine

Article Title: Association between anti-nephrin autoantibodies and disease activity in minimal change disease

doi: 10.1080/07853890.2026.2661115

Figure Lengend Snippet: Serum anti-nephrin autoantibody profiles across disease activity states in patients with MCD. (a) Serum levels of anti-nephrin autoantibodies in patients during active phase, partial remission (PR), and complete remission (CR). (b) Positivity rates of anti-nephrin autoantibodies across disease activity states. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Biotinylated recombinant human nephrin detection protein (17757-H08H, Sino Biological), diluted 1:20,000, was added to the Protein G plates (100 μL/well) and incubated at 37 °C with shaking at 700 rpm for 60 ± 5 min, followed by washing three times with PBST.

Techniques: Activity Assay

Journal: Annals of Medicine

Article Title: Association between anti-nephrin autoantibodies and disease activity in minimal change disease

doi: 10.1080/07853890.2026.2661115

Figure Lengend Snippet: Correlations between serum anti-nephrin autoantibody levels and key clinical parameters.

Article Snippet: Biotinylated recombinant human nephrin detection protein (17757-H08H, Sino Biological), diluted 1:20,000, was added to the Protein G plates (100 μL/well) and incubated at 37 °C with shaking at 700 rpm for 60 ± 5 min, followed by washing three times with PBST.

Techniques:

Journal: Annals of Medicine

Article Title: Association between anti-nephrin autoantibodies and disease activity in minimal change disease

doi: 10.1080/07853890.2026.2661115

Figure Lengend Snippet: Comparison of clinical characteristics between patients positive and negative for anti-nephrin autoantibodies. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Biotinylated recombinant human nephrin detection protein (17757-H08H, Sino Biological), diluted 1:20,000, was added to the Protein G plates (100 μL/well) and incubated at 37 °C with shaking at 700 rpm for 60 ± 5 min, followed by washing three times with PBST.

Techniques: Comparison

Journal: Annals of Medicine

Article Title: Association between anti-nephrin autoantibodies and disease activity in minimal change disease

doi: 10.1080/07853890.2026.2661115

Figure Lengend Snippet: Anti-nephrin autoantibody IgG subclasses in patients with MCD. (a) Proportion (%) of IgG subclasses among anti-nephrin autoantibodies. (b) Correlation of anti‑nephrin-IgG4 with total anti‑nephrin-IgG. (c) Ratios of anti-nephrin-IgG subclasses indicative of Th1/Th2 skewing: IgG1/IgG4 and IgG3/IgG4.

Article Snippet: Biotinylated recombinant human nephrin detection protein (17757-H08H, Sino Biological), diluted 1:20,000, was added to the Protein G plates (100 μL/well) and incubated at 37 °C with shaking at 700 rpm for 60 ± 5 min, followed by washing three times with PBST.

Techniques: