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Influence of LX-2 Cells on MDA-MB-231 Cells through CCL5. Note: ( A ) Schematic representation of the cell experiment process; ( B ) CCK-8 experiment assessing changes in cell proliferation in each group; 100 µL of conditioned medium (CM) per well; ( C ) Scratch assay examining cell migration ability in each group with statistical analysis (scale bar: 100 μm); 2 mL of CM per well; ( D ) Transwell assay measuring cell invasion ability in each group (scale bar: 50 μm); 200 µL of CM per well; ( E ) WB analysis of EMT marker proteins E-cadherin, N-cadherin, and Vimentin, <t>and</t> <t>PI3K-AKT</t> pathway protein expression changes; 2 mL of CM per well. CM: condition medium.* P < 0.05, with all cell experiments repeated three times
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Influence of LX-2 Cells on MDA-MB-231 Cells through CCL5. Note: ( A ) Schematic representation of the cell experiment process; ( B ) CCK-8 experiment assessing changes in cell proliferation in each group; 100 µL of conditioned medium (CM) per well; ( C ) Scratch assay examining cell migration ability in each group with statistical analysis (scale bar: 100 μm); 2 mL of CM per well; ( D ) Transwell assay measuring cell invasion ability in each group (scale bar: 50 μm); 200 µL of CM per well; ( E ) WB analysis of EMT marker proteins E-cadherin, N-cadherin, and Vimentin, <t>and</t> <t>PI3K-AKT</t> pathway protein expression changes; 2 mL of CM per well. CM: condition medium.* P < 0.05, with all cell experiments repeated three times
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Influence of LX-2 Cells on MDA-MB-231 Cells through CCL5. Note: ( A ) Schematic representation of the cell experiment process; ( B ) CCK-8 experiment assessing changes in cell proliferation in each group; 100 µL of conditioned medium (CM) per well; ( C ) Scratch assay examining cell migration ability in each group with statistical analysis (scale bar: 100 μm); 2 mL of CM per well; ( D ) Transwell assay measuring cell invasion ability in each group (scale bar: 50 μm); 200 µL of CM per well; ( E ) WB analysis of EMT marker proteins E-cadherin, N-cadherin, and Vimentin, <t>and</t> <t>PI3K-AKT</t> pathway protein expression changes; 2 mL of CM per well. CM: condition medium.* P < 0.05, with all cell experiments repeated three times
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Influence of LX-2 Cells on MDA-MB-231 Cells through CCL5. Note: ( A ) Schematic representation of the cell experiment process; ( B ) CCK-8 experiment assessing changes in cell proliferation in each group; 100 µL of conditioned medium (CM) per well; ( C ) Scratch assay examining cell migration ability in each group with statistical analysis (scale bar: 100 μm); 2 mL of CM per well; ( D ) Transwell assay measuring cell invasion ability in each group (scale bar: 50 μm); 200 µL of CM per well; ( E ) WB analysis of EMT marker proteins E-cadherin, N-cadherin, and Vimentin, <t>and</t> <t>PI3K-AKT</t> pathway protein expression changes; 2 mL of CM per well. CM: condition medium.* P < 0.05, with all cell experiments repeated three times
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Influence of LX-2 Cells on MDA-MB-231 Cells through CCL5. Note: ( A ) Schematic representation of the cell experiment process; ( B ) CCK-8 experiment assessing changes in cell proliferation in each group; 100 µL of conditioned medium (CM) per well; ( C ) Scratch assay examining cell migration ability in each group with statistical analysis (scale bar: 100 μm); 2 mL of CM per well; ( D ) Transwell assay measuring cell invasion ability in each group (scale bar: 50 μm); 200 µL of CM per well; ( E ) WB analysis of EMT marker proteins E-cadherin, N-cadherin, and Vimentin, <t>and</t> <t>PI3K-AKT</t> pathway protein expression changes; 2 mL of CM per well. CM: condition medium.* P < 0.05, with all cell experiments repeated three times
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Influence of LX-2 Cells on MDA-MB-231 Cells through CCL5. Note: ( A ) Schematic representation of the cell experiment process; ( B ) CCK-8 experiment assessing changes in cell proliferation in each group; 100 µL of conditioned medium (CM) per well; ( C ) Scratch assay examining cell migration ability in each group with statistical analysis (scale bar: 100 μm); 2 mL of CM per well; ( D ) Transwell assay measuring cell invasion ability in each group (scale bar: 50 μm); 200 µL of CM per well; ( E ) WB analysis of EMT marker proteins E-cadherin, N-cadherin, and Vimentin, <t>and</t> <t>PI3K-AKT</t> pathway protein expression changes; 2 mL of CM per well. CM: condition medium.* P < 0.05, with all cell experiments repeated three times
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Influence of LX-2 Cells on MDA-MB-231 Cells through CCL5. Note: ( A ) Schematic representation of the cell experiment process; ( B ) CCK-8 experiment assessing changes in cell proliferation in each group; 100 µL of conditioned medium (CM) per well; ( C ) Scratch assay examining cell migration ability in each group with statistical analysis (scale bar: 100 μm); 2 mL of CM per well; ( D ) Transwell assay measuring cell invasion ability in each group (scale bar: 50 μm); 200 µL of CM per well; ( E ) WB analysis of EMT marker proteins E-cadherin, N-cadherin, and Vimentin, <t>and</t> <t>PI3K-AKT</t> pathway protein expression changes; 2 mL of CM per well. CM: condition medium.* P < 0.05, with all cell experiments repeated three times
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Influence of LX-2 Cells on MDA-MB-231 Cells through CCL5. Note: ( A ) Schematic representation of the cell experiment process; ( B ) CCK-8 experiment assessing changes in cell proliferation in each group; 100 µL of conditioned medium (CM) per well; ( C ) Scratch assay examining cell migration ability in each group with statistical analysis (scale bar: 100 μm); 2 mL of CM per well; ( D ) Transwell assay measuring cell invasion ability in each group (scale bar: 50 μm); 200 µL of CM per well; ( E ) WB analysis of EMT marker proteins E-cadherin, N-cadherin, and Vimentin, <t>and</t> <t>PI3K-AKT</t> pathway protein expression changes; 2 mL of CM per well. CM: condition medium.* P < 0.05, with all cell experiments repeated three times
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Influence of LX-2 Cells on MDA-MB-231 Cells through CCL5. Note: ( A ) Schematic representation of the cell experiment process; ( B ) CCK-8 experiment assessing changes in cell proliferation in each group; 100 µL of conditioned medium (CM) per well; ( C ) Scratch assay examining cell migration ability in each group with statistical analysis (scale bar: 100 μm); 2 mL of CM per well; ( D ) Transwell assay measuring cell invasion ability in each group (scale bar: 50 μm); 200 µL of CM per well; ( E ) WB analysis of EMT marker proteins E-cadherin, N-cadherin, and Vimentin, and PI3K-AKT pathway protein expression changes; 2 mL of CM per well. CM: condition medium.* P < 0.05, with all cell experiments repeated three times

Journal: Cancer Cell International

Article Title: Targeting the CCL5/CCR5-PI3K-AKT axis suppresses hepatic stellate cell-induced breast cancer metastasis

doi: 10.1186/s12935-026-04295-2

Figure Lengend Snippet: Influence of LX-2 Cells on MDA-MB-231 Cells through CCL5. Note: ( A ) Schematic representation of the cell experiment process; ( B ) CCK-8 experiment assessing changes in cell proliferation in each group; 100 µL of conditioned medium (CM) per well; ( C ) Scratch assay examining cell migration ability in each group with statistical analysis (scale bar: 100 μm); 2 mL of CM per well; ( D ) Transwell assay measuring cell invasion ability in each group (scale bar: 50 μm); 200 µL of CM per well; ( E ) WB analysis of EMT marker proteins E-cadherin, N-cadherin, and Vimentin, and PI3K-AKT pathway protein expression changes; 2 mL of CM per well. CM: condition medium.* P < 0.05, with all cell experiments repeated three times

Article Snippet: To investigate the impact of the CCL5, CCR5, and PI3K-AKT signaling pathways on BC cells, during the cell culture process, cells were treated with 10 ng/ml CCL5 (MCE, HY-P7282), 100 nM CCR5 antagonist Maraviroc (MCE, HY-13004), and 500 nM of the PI3K inhibitor BKM120 (MCE, HY-70063) were added to the culture medium [ ].

Techniques: CCK-8 Assay, Wound Healing Assay, Migration, Transwell Assay, Marker, Expressing

Impact of CCR5 and PI3K-AKT Pathway Inhibition on MDA-MB-231 Cells. Note: ( A ) CCK-8 experiment evaluating changes in cell proliferation in each group; ( B ) Scratch assay assessing cell migration ability in each group with statistical representation (scale bar: 100 μm); ( C ) Transwell assay measuring cell invasion ability in each group (scale bar: 50 μm); ( D ) WB analysis of EMT marker proteins E-cadherin, N-cadherin, and Vimentin, and PI3K-AKT pathway protein expression changes. * P < 0.05, with all cell experiments repeated three times

Journal: Cancer Cell International

Article Title: Targeting the CCL5/CCR5-PI3K-AKT axis suppresses hepatic stellate cell-induced breast cancer metastasis

doi: 10.1186/s12935-026-04295-2

Figure Lengend Snippet: Impact of CCR5 and PI3K-AKT Pathway Inhibition on MDA-MB-231 Cells. Note: ( A ) CCK-8 experiment evaluating changes in cell proliferation in each group; ( B ) Scratch assay assessing cell migration ability in each group with statistical representation (scale bar: 100 μm); ( C ) Transwell assay measuring cell invasion ability in each group (scale bar: 50 μm); ( D ) WB analysis of EMT marker proteins E-cadherin, N-cadherin, and Vimentin, and PI3K-AKT pathway protein expression changes. * P < 0.05, with all cell experiments repeated three times

Article Snippet: To investigate the impact of the CCL5, CCR5, and PI3K-AKT signaling pathways on BC cells, during the cell culture process, cells were treated with 10 ng/ml CCL5 (MCE, HY-P7282), 100 nM CCR5 antagonist Maraviroc (MCE, HY-13004), and 500 nM of the PI3K inhibitor BKM120 (MCE, HY-70063) were added to the culture medium [ ].

Techniques: Inhibition, CCK-8 Assay, Wound Healing Assay, Migration, Transwell Assay, Marker, Expressing

CCL5 Derived from HSCs Activates the PI3K-AKT Pathway through the CCL5/CCR5 Axis to Promote the Invasion and Metastasis of Breast Cancer Cells

Journal: Cancer Cell International

Article Title: Targeting the CCL5/CCR5-PI3K-AKT axis suppresses hepatic stellate cell-induced breast cancer metastasis

doi: 10.1186/s12935-026-04295-2

Figure Lengend Snippet: CCL5 Derived from HSCs Activates the PI3K-AKT Pathway through the CCL5/CCR5 Axis to Promote the Invasion and Metastasis of Breast Cancer Cells

Article Snippet: To investigate the impact of the CCL5, CCR5, and PI3K-AKT signaling pathways on BC cells, during the cell culture process, cells were treated with 10 ng/ml CCL5 (MCE, HY-P7282), 100 nM CCR5 antagonist Maraviroc (MCE, HY-13004), and 500 nM of the PI3K inhibitor BKM120 (MCE, HY-70063) were added to the culture medium [ ].

Techniques: Derivative Assay