Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Progesterone boosts abiraterone-driven target and NK cell therapies against glioblastoma

doi: 10.1186/s13046-024-03144-2

Figure Lengend Snippet: Effect of Prog on MGMT expression. A , B After treatment with Prog in the absence and presence of Abi, T98G cells were harvested for Western blotting (Left) and RT-qPCR (Right). C Left: After treatment for 48 h, T98G cells were treated with 20 µM cycloheximide (CHX) for the indicated time interval, and cell lysates were collected for Western blotting. Right: Quantitative results. D After treatment with Prog, gDNA of T98G and U87MG-R cells F was purified, and subjected to pyrosequencing analysis. The percentage of methylation in 5 CpG residues in T98G and U87MG-R cells were quantified. Experiments were performed independently three times. (* P < 0.05, ** P < 0.01)

Article Snippet: CpGs methylation are tested with the PyroMark Q24 CpG MGMT kit provided by Qiagen [ , ].

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Purification, Methylation

Journal: Cancers

Article Title: ‘The Reports of My Death Are Greatly Exaggerated’—Evaluating the Effect of Necrosis on MGMT Promoter Methylation Testing in High-Grade Glioma

doi: 10.3390/cancers16101906

Figure Lengend Snippet: Overview of intratumoral cellularity, necrosis and MGMT p methylation percentages across 52 primary HGG cases with repeated MGMT p testing. Horizontal red lines indicate the borderline MGMT p zone (9–13%).

Article Snippet: Pyrosequencing of the MGMT p region was undertaken using a PyroMark therascreen MGMT kit (Qiagen) and the PyroMark Q24 system (Qiagen), which detect four CpG sites located in exon 1.

Techniques: Methylation

Journal: Cancers

Article Title: ‘The Reports of My Death Are Greatly Exaggerated’—Evaluating the Effect of Necrosis on MGMT Promoter Methylation Testing in High-Grade Glioma

doi: 10.3390/cancers16101906

Figure Lengend Snippet: Summary of primary and recurrent presentations of HGGs with maintenance of MGMT p status.

Article Snippet: Pyrosequencing of the MGMT p region was undertaken using a PyroMark therascreen MGMT kit (Qiagen) and the PyroMark Q24 system (Qiagen), which detect four CpG sites located in exon 1.

Techniques: Blocking Assay, Methylation

Journal: Cancers

Article Title: ‘The Reports of My Death Are Greatly Exaggerated’—Evaluating the Effect of Necrosis on MGMT Promoter Methylation Testing in High-Grade Glioma

doi: 10.3390/cancers16101906

Figure Lengend Snippet: Overview of intratumoral cellularity, necrosis and MGMT p methylation percentages in 23 cases of recurrent HGG. Horizontal red lines indicate the borderline MGMT p zone (9–13%).

Article Snippet: Pyrosequencing of the MGMT p region was undertaken using a PyroMark therascreen MGMT kit (Qiagen) and the PyroMark Q24 system (Qiagen), which detect four CpG sites located in exon 1.

Techniques: Methylation

Journal: Cancers

Article Title: ‘The Reports of My Death Are Greatly Exaggerated’—Evaluating the Effect of Necrosis on MGMT Promoter Methylation Testing in High-Grade Glioma

doi: 10.3390/cancers16101906

Figure Lengend Snippet: Correlation analyses demonstrate the reproducibility of MGMT p methylation testing in HGG sections with varying degrees of tumor necrosis. Repeated measures correlation analysis shows no significant association between MGMT p status and tumor cellularity ( A ) or necrosis ( B ). Each participant, plotted in a different color, contributes two paired data points representing repeated MGMT p tests on different tissue blocks. The corresponding lines depict the repeated measures correlation model. The relationship of (black) line-of-best-fit between MGMT p methylation percentages (data points depicted by red triangles) at thresholds of 30% necrosis and 60% tumor cellularity was determined by Spearman rank correlation ( C ).

Article Snippet: Pyrosequencing of the MGMT p region was undertaken using a PyroMark therascreen MGMT kit (Qiagen) and the PyroMark Q24 system (Qiagen), which detect four CpG sites located in exon 1.

Techniques: Methylation

Journal: Cancers

Article Title: ‘The Reports of My Death Are Greatly Exaggerated’—Evaluating the Effect of Necrosis on MGMT Promoter Methylation Testing in High-Grade Glioma

doi: 10.3390/cancers16101906

Figure Lengend Snippet: Changes in MGMT p status by degree of necrosis in NT block.

Article Snippet: Pyrosequencing of the MGMT p region was undertaken using a PyroMark therascreen MGMT kit (Qiagen) and the PyroMark Q24 system (Qiagen), which detect four CpG sites located in exon 1.

Techniques: Blocking Assay

Journal: Cancers

Article Title: ‘The Reports of My Death Are Greatly Exaggerated’—Evaluating the Effect of Necrosis on MGMT Promoter Methylation Testing in High-Grade Glioma

doi: 10.3390/cancers16101906

Figure Lengend Snippet: Overview of tumoral cellularity, necrosis and MGMT p methylation percentages in 19 cases of recurrent HGG (16 recurrences; 3 re-resected recurrences). Horizontal red lines indicate the borderline MGMT p zone (9–13%).

Article Snippet: Pyrosequencing of the MGMT p region was undertaken using a PyroMark therascreen MGMT kit (Qiagen) and the PyroMark Q24 system (Qiagen), which detect four CpG sites located in exon 1.

Techniques: Methylation