Journal: International Journal of General Medicine

Article Title: A POSTN + CAF/APOE + TAM Axis is Associated with Tumor Progression and Potential Immunotherapy Resistance in Laryngeal Squamous Cell Carcinoma

doi: 10.2147/IJGM.S583478

Figure Lengend Snippet: Intercellular communication between POSTN + CAFs and APOE + TAMs in LSCC. ( A ) Global ligand–receptor interaction networks reveal strong bidirectional communication between POSTN + CAFs and APOE + TAMs. ( B ) Outgoing signaling patterns from POSTN + CAFs. ( C ) Outgoing signaling patterns from APOE + TAMs. (In figure A-C, the numbers on the edges indicate the amount of inferred significant intercellular interactions between cell populations.) ( D ) Bubble plots highlight significant ligand–receptor pairs mediating communication from POSTN + CAFs. ( E ) GO enrichment indicates chemotaxis and apoptotic signaling modulation as major biological processes (from POSTN + CAFs to APOE + TAMs). ( F ) KEGG analysis identifies cytokine-cytokine receptor interactions, complement/coagulation as dominant pathways (from POSTN + CAFs to APOE + TAMs). ( G ) Bubble plots highlight significant ligand–receptor pairs mediating communication from APOE + TAMs. ( H ) GO enrichment indicates chemotaxis and apoptotic signaling modulation as major biological processes (from APOE + TAMs to POSTN + CAFs). ( I ) KEGG analysis identifies proteoglycans in cancer, cytokine-cytokine receptor interaction as dominant pathways (from APOE + TAMs to POSTN + CAFs).

Article Snippet: Sections were then incubated with primary antibodies against POSTN (Epizyme Biotech, catalog number: R014951) and APOE (Epizyme Biotech, catalog number: R013930), followed by FITC- and Cy3-conjugated secondary antibodies.

Techniques: Chemotaxis Assay, Coagulation

Journal: International Journal of General Medicine

Article Title: A POSTN + CAF/APOE + TAM Axis is Associated with Tumor Progression and Potential Immunotherapy Resistance in Laryngeal Squamous Cell Carcinoma

doi: 10.2147/IJGM.S583478

Figure Lengend Snippet: Correlation and spatial colocalization between POSTN + CAFs and APOE + TAMs in LSCC. ( A – C ) Scatter plots showing the positive correlation between POSTN + CAF abundance and APOE + TAM abundance across multiple cohorts, including TCGA-HNSC, GSE41613 , and GSE65858 . Shaded areas represent 95% confidence intervals, and the correlation was evaluated using Spearman’s test. ( D ) Representative multiplex immunofluorescence staining images of LSCC tissues showing colocalization of POSTN (red) and APOE (green) proteins. DAPI (blue) marks cell nuclei.

Article Snippet: Sections were then incubated with primary antibodies against POSTN (Epizyme Biotech, catalog number: R014951) and APOE (Epizyme Biotech, catalog number: R013930), followed by FITC- and Cy3-conjugated secondary antibodies.

Techniques: Multiplex Assay, Immunofluorescence, Staining

Journal: International Journal of General Medicine

Article Title: A POSTN + CAF/APOE + TAM Axis is Associated with Tumor Progression and Potential Immunotherapy Resistance in Laryngeal Squamous Cell Carcinoma

doi: 10.2147/IJGM.S583478

Figure Lengend Snippet: Protein–protein interaction between POSTN and APOE. ( A ) Structural model of the POSTN-APOE complex; the inset highlights key hydrogen-bonding and hydrophobic interactions. ( B and C ) Electrostatic potential maps of POSTN ( B ) and APOE ( C ), showing charge complementarity at the interface. ( D ) A 2D interaction diagram illustrating hydrogen bonds and hydrophobic contacts between key residues. Symbols used: red lines for hydrogen bonds, blue circles for non-ligand bonds, and pink arcs for hydrophobic contacts.

Article Snippet: Sections were then incubated with primary antibodies against POSTN (Epizyme Biotech, catalog number: R014951) and APOE (Epizyme Biotech, catalog number: R013930), followed by FITC- and Cy3-conjugated secondary antibodies.

Techniques:

Journal: International Journal of General Medicine

Article Title: A POSTN + CAF/APOE + TAM Axis is Associated with Tumor Progression and Potential Immunotherapy Resistance in Laryngeal Squamous Cell Carcinoma

doi: 10.2147/IJGM.S583478

Figure Lengend Snippet: Prognostic and functional implications of POSTN + CAFs and APOE + TAMs co-enrichment. ( A ) Kaplan-Meier survival analysis shows the worst outcomes in the POSTN + CAFs high /APOE + TAMs high group. ( B ) GO analysis reveals enrichment in extracellular matrix organization and collagen regulation in the POSTN + CAFs high /APOE + TAMs high group. ( C ) GSEA indicates activation of EMT, antigen presentation, neuroactive ligand–receptor signaling, and TGF-β pathways in the POSTN + CAFs high /APOE + TAMs high group.

Article Snippet: Sections were then incubated with primary antibodies against POSTN (Epizyme Biotech, catalog number: R014951) and APOE (Epizyme Biotech, catalog number: R013930), followed by FITC- and Cy3-conjugated secondary antibodies.

Techniques: Functional Assay, Activation Assay, Immunopeptidomics

Journal: International Journal of General Medicine

Article Title: A POSTN + CAF/APOE + TAM Axis is Associated with Tumor Progression and Potential Immunotherapy Resistance in Laryngeal Squamous Cell Carcinoma

doi: 10.2147/IJGM.S583478

Figure Lengend Snippet: Association of the POSTN + CAFs/APOE + TAMs axis with immune suppression and ICI resistance. ( A ) TME immune cell infiltration across LSCC clinical subgroups quantified by five deconvolution algorithms. ( B ) High POSTN + CAFs/APOE + TAMs enrichment LSCC shows increased stromal and immune scores but reduced tumor purity, as well as TIDE scores with a tendency to be higher. ( C – E ) The IMvigor210 cohort validates poorer survival in POSTN + CAFs high /APOE + TAMs high patients. ( F ) Treatment-response comparison shows a higher proportion of PD/SD in the high-risk group ( χ² -test).

Article Snippet: Sections were then incubated with primary antibodies against POSTN (Epizyme Biotech, catalog number: R014951) and APOE (Epizyme Biotech, catalog number: R013930), followed by FITC- and Cy3-conjugated secondary antibodies.

Techniques: Comparison

Journal: Bioactive Materials

Article Title: Engineered atopic dermatitis models for recreating hypoxic conditions in atopic dermatitis microenvironments

doi: 10.1016/j.bioactmat.2025.12.045

Figure Lengend Snippet: Neuroregulatory role of AD-specific COL6A5+ FBs. (a) Heatmap of summarized GO terms enriched in COL6A5+ FBs. (b) Heatmap of summarized GO terms overrepresented in selected regulons responsible for neuro-regulation (TCF4_extended and TFAP2C_extended). (c) Inferred LR pairs mediating interaction between COL6A5+ FBs and sensory neurons in the skin. (d) Dot plot of the POSTN expression in FB subtypes.

Article Snippet: Primers for glyceraldehyde-3-phosphate dehydrogenase (GAPDH), HIF-1α, periostin (POSTN), tenascin-C (TNC), Wnt family member 5 A (WNT5A), and COL6A5 were supplied by Cosmogenetech (Seongdong-gu, Seoul).

Techniques: Expressing

Journal: Bioactive Materials

Article Title: Engineered atopic dermatitis models for recreating hypoxic conditions in atopic dermatitis microenvironments

doi: 10.1016/j.bioactmat.2025.12.045

Figure Lengend Snippet: Analysis of periostin expression. (a) Schematic representations of periostin expression analysis. Gene expression under (b) 21 % p O 2 condition and (c) 5 % p O 2 condition (qRT-PCR). (d) Immunofluorescence staining of periostin. (e) Protein expression (ELISA) analysis. (f) Volcano plot comparing FBs under hypoxia without IL-4 (down – blue) and hypoxia with IL-4 (up – red). Labeled genes are those known to be associated with AD pathogenesis and meet the DEG criteria (padj < 0.05 & log2FC > 0). (g) Normalized expression heatmap of selected genes associated with AD pathogenesis. Data are presented as the mean ± s.d. (b, c, e) (n = 3–5). Statistical significance and p values are determined by one-way ANOVA with Tukey's post-hoc comparison (b, c) and unpaired two-tailed Student's t-test (e). (∗) indicates a significant difference (∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001). (N.D.; Not detected).

Article Snippet: Primers for glyceraldehyde-3-phosphate dehydrogenase (GAPDH), HIF-1α, periostin (POSTN), tenascin-C (TNC), Wnt family member 5 A (WNT5A), and COL6A5 were supplied by Cosmogenetech (Seongdong-gu, Seoul).

Techniques: Expressing, Gene Expression, Quantitative RT-PCR, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Labeling, Comparison, Two Tailed Test

Journal: The World Journal of Men's Health

Article Title: Comprehensive Analysis of N6-Methyladenosine Modification Profiling in Diabetic Erectile Dysfunction

doi: 10.5534/wjmh.240328

Figure Lengend Snippet: Subpopulation distribution of hub genes. (A) Clustering visualization of subpopulations in the corpus cavernosum. (B) Expression distribution of hub genes in different cells of corpus cavernosum. The expression distribution of POSTN (C, D) and LOX (E, F) in the tissue of corpus cavernosum. UMAP: uniform manifold approximation and projection, EC: endothelial cell, FB: fibroblast, SMC: smooth muscle cell, SWC: Schwann cell, MAC: macrophage, T: T cell.

Article Snippet: Similarly, the sections of corpus cavernosum were subjected to immunohistochemistry to detect the protein expression of POSTN (66491-1-Ig, Proteintech) and LOX (A11504, ABclonal).

Techniques: Expressing