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pUS2 suppresses IFN-β production in DEF cells. A. DEF cells were co-transfected with the IFN-β luciferase reporter plasmid, Renilla luciferase control plasmid, and either pUS2 expression plasmid or empty vector. Cells were stimulated with <t>poly(I:C),</t> and IFN-β promoter activity was measured using a dual-luciferase reporter assay. pUL45, a DPV viral protein, was used as a negative control. B. DEF cells were co-transfected as described above and stimulated with poly(dA:dT). IFN-β promoter activity was measured. C. The mRNA expression levels of IFN-β, OASL, and Mx in DEF cells following poly(I:C) stimulation in the presence or absence of pUS2 were determined by RT-qPCR. D. Expression of pUS2 protein was confirmed by Western blotting analysis.
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pUS2 suppresses IFN-β production in DEF cells. A. DEF cells were co-transfected with the IFN-β luciferase reporter plasmid, Renilla luciferase control plasmid, and either pUS2 expression plasmid or empty vector. Cells were stimulated with poly(I:C), and IFN-β promoter activity was measured using a dual-luciferase reporter assay. pUL45, a DPV viral protein, was used as a negative control. B. DEF cells were co-transfected as described above and stimulated with poly(dA:dT). IFN-β promoter activity was measured. C. The mRNA expression levels of IFN-β, OASL, and Mx in DEF cells following poly(I:C) stimulation in the presence or absence of pUS2 were determined by RT-qPCR. D. Expression of pUS2 protein was confirmed by Western blotting analysis.

Journal: Poultry Science

Article Title: Duck plague virus US2 promotes p62-mediated autophagic degradation of RIG-I to suppress antiviral signaling

doi: 10.1016/j.psj.2026.107155

Figure Lengend Snippet: pUS2 suppresses IFN-β production in DEF cells. A. DEF cells were co-transfected with the IFN-β luciferase reporter plasmid, Renilla luciferase control plasmid, and either pUS2 expression plasmid or empty vector. Cells were stimulated with poly(I:C), and IFN-β promoter activity was measured using a dual-luciferase reporter assay. pUL45, a DPV viral protein, was used as a negative control. B. DEF cells were co-transfected as described above and stimulated with poly(dA:dT). IFN-β promoter activity was measured. C. The mRNA expression levels of IFN-β, OASL, and Mx in DEF cells following poly(I:C) stimulation in the presence or absence of pUS2 were determined by RT-qPCR. D. Expression of pUS2 protein was confirmed by Western blotting analysis.

Article Snippet: Cells were stimulated with poly(I:C) (MCE, Shanghai, China; Cat# HY-107202) or poly(dA:dT) (Sigma-Aldrich, St. Louis, MO, USA; Cat# P9764) and simultaneously transfected with either the empty vector or the US2 expression plasmid.

Techniques: Transfection, Luciferase, Plasmid Preparation, Control, Expressing, Activity Assay, Reporter Assay, Negative Control, Quantitative RT-PCR, Western Blot