Review





Similar Products

pmirglo dualluciferase reporter vectors  (Shanghai Genechem Ltd)
86
Shanghai Genechem Ltd pmirglo dualluciferase reporter vectors
Pmirglo Dualluciferase Reporter Vectors, supplied by Shanghai Genechem Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmirglo dualluciferase reporter vectors/product/Shanghai Genechem Ltd
Average 86 stars, based on 1 article reviews
pmirglo dualluciferase reporter vectors - by Bioz Stars, 2026-05
86/100 stars
  Buy from Supplier
iv 80 pmirglo vector  (Addgene inc)
93
Addgene inc iv 80 pmirglo vector
Iv 80 Pmirglo Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iv 80 pmirglo vector/product/Addgene inc
Average 93 stars, based on 1 article reviews
iv 80 pmirglo vector - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
pmirglo luciferase vectors  (Sangon Biotech)
86
Sangon Biotech pmirglo luciferase vectors
Pmirglo Luciferase Vectors, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmirglo luciferase vectors/product/Sangon Biotech
Average 86 stars, based on 1 article reviews
pmirglo luciferase vectors - by Bioz Stars, 2026-05
86/100 stars
  Buy from Supplier
pmirglo vectors (wt or mut)  (Promega)
90
Promega pmirglo vectors (wt or mut)
Pmirglo Vectors (Wt Or Mut), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmirglo vectors (wt or mut)/product/Promega
Average 90 stars, based on 1 article reviews
pmirglo vectors (wt or mut) - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
pmirglo luciferase reporter vectors  (Promega)
90
Promega pmirglo luciferase reporter vectors
Pmirglo Luciferase Reporter Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmirglo luciferase reporter vectors/product/Promega
Average 90 stars, based on 1 article reviews
pmirglo luciferase reporter vectors - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
pmirglo vectors  (Promega)
90
Promega pmirglo vectors
Sponging relationship between BACE1-AS <t>and</t> <t>miR-6805-5p</t> in HCT-8 cells during C. parvum infection. (A) Subcellular location of BACE1-AS in HCT-8 cells. HCT-8 cells were seeded on a coverslip and were infected with C. parvum for 24 h and then applied for FISH analysis of BACE1-AS using probes conjugated with cy3, with 18S localized in the cytoplasm as a control. The nucleus was visualized by DAPI staining. (B) The mRNA levels of miR-6805-5p in HCT-8 cells transfected with pcDNA3.1(+)-BACE1-AS plasmid or si-BACE1-AS following C. parvum infection at 24 hpi by using RT-qPCR. (C) Putative binding site between BACE1-AS and miR-6805-5p predicted by LncBook. (D) The luciferase activity in HCT-8 cells co-transfected with miR-6805-5p mimics and <t>pmirGLO-BACE1-AS-WT</t> or pmirGLO-BACE1-AS-MUT. Statistical analysis was conducted by using a non-parametric t -test. * P < 0.05, ** P < 0.01. WT, wild type.
Pmirglo Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmirglo vectors/product/Promega
Average 90 stars, based on 1 article reviews
pmirglo vectors - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
pmirglo dual luciferase vectors  (Promega)
90
Promega pmirglo dual luciferase vectors
Sponging relationship between BACE1-AS <t>and</t> <t>miR-6805-5p</t> in HCT-8 cells during C. parvum infection. (A) Subcellular location of BACE1-AS in HCT-8 cells. HCT-8 cells were seeded on a coverslip and were infected with C. parvum for 24 h and then applied for FISH analysis of BACE1-AS using probes conjugated with cy3, with 18S localized in the cytoplasm as a control. The nucleus was visualized by DAPI staining. (B) The mRNA levels of miR-6805-5p in HCT-8 cells transfected with pcDNA3.1(+)-BACE1-AS plasmid or si-BACE1-AS following C. parvum infection at 24 hpi by using RT-qPCR. (C) Putative binding site between BACE1-AS and miR-6805-5p predicted by LncBook. (D) The luciferase activity in HCT-8 cells co-transfected with miR-6805-5p mimics and <t>pmirGLO-BACE1-AS-WT</t> or pmirGLO-BACE1-AS-MUT. Statistical analysis was conducted by using a non-parametric t -test. * P < 0.05, ** P < 0.01. WT, wild type.
Pmirglo Dual Luciferase Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmirglo dual luciferase vectors/product/Promega
Average 90 stars, based on 1 article reviews
pmirglo dual luciferase vectors - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
pmirglo vectors  (Shanghai GenePharma)
90
Shanghai GenePharma pmirglo vectors
Sponging relationship between BACE1-AS <t>and</t> <t>miR-6805-5p</t> in HCT-8 cells during C. parvum infection. (A) Subcellular location of BACE1-AS in HCT-8 cells. HCT-8 cells were seeded on a coverslip and were infected with C. parvum for 24 h and then applied for FISH analysis of BACE1-AS using probes conjugated with cy3, with 18S localized in the cytoplasm as a control. The nucleus was visualized by DAPI staining. (B) The mRNA levels of miR-6805-5p in HCT-8 cells transfected with pcDNA3.1(+)-BACE1-AS plasmid or si-BACE1-AS following C. parvum infection at 24 hpi by using RT-qPCR. (C) Putative binding site between BACE1-AS and miR-6805-5p predicted by LncBook. (D) The luciferase activity in HCT-8 cells co-transfected with miR-6805-5p mimics and <t>pmirGLO-BACE1-AS-WT</t> or pmirGLO-BACE1-AS-MUT. Statistical analysis was conducted by using a non-parametric t -test. * P < 0.05, ** P < 0.01. WT, wild type.
Pmirglo Vectors, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmirglo vectors/product/Shanghai GenePharma
Average 90 stars, based on 1 article reviews
pmirglo vectors - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
pmirglo dual-luciferase reporter vectors  (Promega)
90
Promega pmirglo dual-luciferase reporter vectors
Sponging relationship between BACE1-AS <t>and</t> <t>miR-6805-5p</t> in HCT-8 cells during C. parvum infection. (A) Subcellular location of BACE1-AS in HCT-8 cells. HCT-8 cells were seeded on a coverslip and were infected with C. parvum for 24 h and then applied for FISH analysis of BACE1-AS using probes conjugated with cy3, with 18S localized in the cytoplasm as a control. The nucleus was visualized by DAPI staining. (B) The mRNA levels of miR-6805-5p in HCT-8 cells transfected with pcDNA3.1(+)-BACE1-AS plasmid or si-BACE1-AS following C. parvum infection at 24 hpi by using RT-qPCR. (C) Putative binding site between BACE1-AS and miR-6805-5p predicted by LncBook. (D) The luciferase activity in HCT-8 cells co-transfected with miR-6805-5p mimics and <t>pmirGLO-BACE1-AS-WT</t> or pmirGLO-BACE1-AS-MUT. Statistical analysis was conducted by using a non-parametric t -test. * P < 0.05, ** P < 0.01. WT, wild type.
Pmirglo Dual Luciferase Reporter Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmirglo dual-luciferase reporter vectors/product/Promega
Average 90 stars, based on 1 article reviews
pmirglo dual-luciferase reporter vectors - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

Image Search Results


Sponging relationship between BACE1-AS and miR-6805-5p in HCT-8 cells during C. parvum infection. (A) Subcellular location of BACE1-AS in HCT-8 cells. HCT-8 cells were seeded on a coverslip and were infected with C. parvum for 24 h and then applied for FISH analysis of BACE1-AS using probes conjugated with cy3, with 18S localized in the cytoplasm as a control. The nucleus was visualized by DAPI staining. (B) The mRNA levels of miR-6805-5p in HCT-8 cells transfected with pcDNA3.1(+)-BACE1-AS plasmid or si-BACE1-AS following C. parvum infection at 24 hpi by using RT-qPCR. (C) Putative binding site between BACE1-AS and miR-6805-5p predicted by LncBook. (D) The luciferase activity in HCT-8 cells co-transfected with miR-6805-5p mimics and pmirGLO-BACE1-AS-WT or pmirGLO-BACE1-AS-MUT. Statistical analysis was conducted by using a non-parametric t -test. * P < 0.05, ** P < 0.01. WT, wild type.

Journal: Microbiology Spectrum

Article Title: LncRNA BACE1-AS delays the propagation of Cryptosporidium parvum through regulating cell apoptosis by targeting the miR-6805-5p/IRF3 axis

doi: 10.1128/spectrum.02022-24

Figure Lengend Snippet: Sponging relationship between BACE1-AS and miR-6805-5p in HCT-8 cells during C. parvum infection. (A) Subcellular location of BACE1-AS in HCT-8 cells. HCT-8 cells were seeded on a coverslip and were infected with C. parvum for 24 h and then applied for FISH analysis of BACE1-AS using probes conjugated with cy3, with 18S localized in the cytoplasm as a control. The nucleus was visualized by DAPI staining. (B) The mRNA levels of miR-6805-5p in HCT-8 cells transfected with pcDNA3.1(+)-BACE1-AS plasmid or si-BACE1-AS following C. parvum infection at 24 hpi by using RT-qPCR. (C) Putative binding site between BACE1-AS and miR-6805-5p predicted by LncBook. (D) The luciferase activity in HCT-8 cells co-transfected with miR-6805-5p mimics and pmirGLO-BACE1-AS-WT or pmirGLO-BACE1-AS-MUT. Statistical analysis was conducted by using a non-parametric t -test. * P < 0.05, ** P < 0.01. WT, wild type.

Article Snippet: In this study, the luciferase reporter plasmids of wild-type BACE1-AS (BACE1-AS -WT), mutant BACE1-AS (BACE1-AS -MUT), wild-type IRF3 ( IRF3 -WT), and mutant IRF3 ( IRF3 -MUT) were constructed by cloning BACE1-AS, the miR-6805-5p binding site-mutated BACE1-AS, 3′ UTR region of IRF3 , and the miR-6805-5p binding site-mutated 3′ UTR region of IRF3 into pmirGLO vectors (Promega, Madison, WI, USA), respectively.

Techniques: Infection, Control, Staining, Transfection, Plasmid Preparation, Quantitative RT-PCR, Binding Assay, Luciferase, Activity Assay

Target relationship between miR-6805-5p and IRF3 in HCT-8 cells following C. parvum infection. The mRNA (A) and protein (B) levels of IRF3 in HCT-8 cells transfected with miR-6805-5p mimics or inhibitor following C. parvum infection at 24 hpi by RT-qPCR and Western blot. ImageJ was used to calculate the protein level normalized to GAPDH by densitometry. (C) Putative binding site between the 3′-UTR of IRF3 and miR-6805-5p predicted by miRWalk. (D) The luciferase activity in HCT-8 cells co-transfected with miR-6805-5p mimics and pmirGLO- IRF3 -WT or pmirGLO- IRF3 -MUT. Three independent experiments were performed. Statistical analysis was conducted by using a non-parametric t- test. * P < 0.05.

Journal: Microbiology Spectrum

Article Title: LncRNA BACE1-AS delays the propagation of Cryptosporidium parvum through regulating cell apoptosis by targeting the miR-6805-5p/IRF3 axis

doi: 10.1128/spectrum.02022-24

Figure Lengend Snippet: Target relationship between miR-6805-5p and IRF3 in HCT-8 cells following C. parvum infection. The mRNA (A) and protein (B) levels of IRF3 in HCT-8 cells transfected with miR-6805-5p mimics or inhibitor following C. parvum infection at 24 hpi by RT-qPCR and Western blot. ImageJ was used to calculate the protein level normalized to GAPDH by densitometry. (C) Putative binding site between the 3′-UTR of IRF3 and miR-6805-5p predicted by miRWalk. (D) The luciferase activity in HCT-8 cells co-transfected with miR-6805-5p mimics and pmirGLO- IRF3 -WT or pmirGLO- IRF3 -MUT. Three independent experiments were performed. Statistical analysis was conducted by using a non-parametric t- test. * P < 0.05.

Article Snippet: In this study, the luciferase reporter plasmids of wild-type BACE1-AS (BACE1-AS -WT), mutant BACE1-AS (BACE1-AS -MUT), wild-type IRF3 ( IRF3 -WT), and mutant IRF3 ( IRF3 -MUT) were constructed by cloning BACE1-AS, the miR-6805-5p binding site-mutated BACE1-AS, 3′ UTR region of IRF3 , and the miR-6805-5p binding site-mutated 3′ UTR region of IRF3 into pmirGLO vectors (Promega, Madison, WI, USA), respectively.

Techniques: Infection, Transfection, Quantitative RT-PCR, Western Blot, Binding Assay, Luciferase, Activity Assay