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phospholipase c plc inhibitor u73122  (MedChemExpress)
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The effects of <t>U73122</t> and glucose on BoAHV-1 entry process. ( A and B ) MDBK ( A ) and A549 ( B ) cells in six-well plates were subjected to glucose depletion via culturing the cells using glucose-free DMEM for 12 h. The cells were infected with BoAHV-1 (MOI = 1) in the presence of either U73122 or D-glucose alone, or in combination, at the designated concentrations, at 37°C for 30 min. After extensive washes, genomic DNA was extracted, and the viral genome bound to the cells was quantitatively evaluated using qPCR. The data shown are the means of three independent experiments with error bars representing the SDs. The significance was evaluated by Student’s t -test (*, P < 0.05; **, P < 0.01).
Phospholipase C Plc Inhibitor U73122, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The effects of <t>U73122</t> and glucose on BoAHV-1 entry process. ( A and B ) MDBK ( A ) and A549 ( B ) cells in six-well plates were subjected to glucose depletion via culturing the cells using glucose-free DMEM for 12 h. The cells were infected with BoAHV-1 (MOI = 1) in the presence of either U73122 or D-glucose alone, or in combination, at the designated concentrations, at 37°C for 30 min. After extensive washes, genomic DNA was extracted, and the viral genome bound to the cells was quantitatively evaluated using qPCR. The data shown are the means of three independent experiments with error bars representing the SDs. The significance was evaluated by Student’s t -test (*, P < 0.05; **, P < 0.01).
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plc inhibitor u73122  (Tocris)
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The effects of <t>U73122</t> and glucose on BoAHV-1 entry process. ( A and B ) MDBK ( A ) and A549 ( B ) cells in six-well plates were subjected to glucose depletion via culturing the cells using glucose-free DMEM for 12 h. The cells were infected with BoAHV-1 (MOI = 1) in the presence of either U73122 or D-glucose alone, or in combination, at the designated concentrations, at 37°C for 30 min. After extensive washes, genomic DNA was extracted, and the viral genome bound to the cells was quantitatively evaluated using qPCR. The data shown are the means of three independent experiments with error bars representing the SDs. The significance was evaluated by Student’s t -test (*, P < 0.05; **, P < 0.01).
Plc Inhibitor U73122, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plc inhibitor u 73122  (Fisher Scientific)
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The effects of <t>U73122</t> and glucose on BoAHV-1 entry process. ( A and B ) MDBK ( A ) and A549 ( B ) cells in six-well plates were subjected to glucose depletion via culturing the cells using glucose-free DMEM for 12 h. The cells were infected with BoAHV-1 (MOI = 1) in the presence of either U73122 or D-glucose alone, or in combination, at the designated concentrations, at 37°C for 30 min. After extensive washes, genomic DNA was extracted, and the viral genome bound to the cells was quantitatively evaluated using qPCR. The data shown are the means of three independent experiments with error bars representing the SDs. The significance was evaluated by Student’s t -test (*, P < 0.05; **, P < 0.01).
Plc Inhibitor U 73122, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospholi pase c plc inhibitor u73122  (MedChemExpress)
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MedChemExpress phospholi pase c plc inhibitor u73122
The effects of <t>U73122</t> and glucose on BoAHV-1 entry process. ( A and B ) MDBK ( A ) and A549 ( B ) cells in six-well plates were subjected to glucose depletion via culturing the cells using glucose-free DMEM for 12 h. The cells were infected with BoAHV-1 (MOI = 1) in the presence of either U73122 or D-glucose alone, or in combination, at the designated concentrations, at 37°C for 30 min. After extensive washes, genomic DNA was extracted, and the viral genome bound to the cells was quantitatively evaluated using qPCR. The data shown are the means of three independent experiments with error bars representing the SDs. The significance was evaluated by Student’s t -test (*, P < 0.05; **, P < 0.01).
Phospholi Pase C Plc Inhibitor U73122, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plc inhibitor u73122  (Adipogen)
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Adipogen plc inhibitor u73122
The effects of <t>U73122</t> and glucose on BoAHV-1 entry process. ( A and B ) MDBK ( A ) and A549 ( B ) cells in six-well plates were subjected to glucose depletion via culturing the cells using glucose-free DMEM for 12 h. The cells were infected with BoAHV-1 (MOI = 1) in the presence of either U73122 or D-glucose alone, or in combination, at the designated concentrations, at 37°C for 30 min. After extensive washes, genomic DNA was extracted, and the viral genome bound to the cells was quantitatively evaluated using qPCR. The data shown are the means of three independent experiments with error bars representing the SDs. The significance was evaluated by Student’s t -test (*, P < 0.05; **, P < 0.01).
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plc inhibitor  (Tocris)
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Tocris plc inhibitor
The effects of <t>U73122</t> and glucose on BoAHV-1 entry process. ( A and B ) MDBK ( A ) and A549 ( B ) cells in six-well plates were subjected to glucose depletion via culturing the cells using glucose-free DMEM for 12 h. The cells were infected with BoAHV-1 (MOI = 1) in the presence of either U73122 or D-glucose alone, or in combination, at the designated concentrations, at 37°C for 30 min. After extensive washes, genomic DNA was extracted, and the viral genome bound to the cells was quantitatively evaluated using qPCR. The data shown are the means of three independent experiments with error bars representing the SDs. The significance was evaluated by Student’s t -test (*, P < 0.05; **, P < 0.01).
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plc inhibitor d609  (MedChemExpress)
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MedChemExpress plc inhibitor d609
The effects of <t>U73122</t> and glucose on BoAHV-1 entry process. ( A and B ) MDBK ( A ) and A549 ( B ) cells in six-well plates were subjected to glucose depletion via culturing the cells using glucose-free DMEM for 12 h. The cells were infected with BoAHV-1 (MOI = 1) in the presence of either U73122 or D-glucose alone, or in combination, at the designated concentrations, at 37°C for 30 min. After extensive washes, genomic DNA was extracted, and the viral genome bound to the cells was quantitatively evaluated using qPCR. The data shown are the means of three independent experiments with error bars representing the SDs. The significance was evaluated by Student’s t -test (*, P < 0.05; **, P < 0.01).
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The effects of U73122 and glucose on BoAHV-1 entry process. ( A and B ) MDBK ( A ) and A549 ( B ) cells in six-well plates were subjected to glucose depletion via culturing the cells using glucose-free DMEM for 12 h. The cells were infected with BoAHV-1 (MOI = 1) in the presence of either U73122 or D-glucose alone, or in combination, at the designated concentrations, at 37°C for 30 min. After extensive washes, genomic DNA was extracted, and the viral genome bound to the cells was quantitatively evaluated using qPCR. The data shown are the means of three independent experiments with error bars representing the SDs. The significance was evaluated by Student’s t -test (*, P < 0.05; **, P < 0.01).

Journal: Microbiology Spectrum

Article Title: D-glucose uptake inhibits bovine alphaherpesvirus 1 post-binding cell process entry via inhibition of PLC-γ1 signaling in a glucose transporter 1-independent manner

doi: 10.1128/spectrum.02456-25

Figure Lengend Snippet: The effects of U73122 and glucose on BoAHV-1 entry process. ( A and B ) MDBK ( A ) and A549 ( B ) cells in six-well plates were subjected to glucose depletion via culturing the cells using glucose-free DMEM for 12 h. The cells were infected with BoAHV-1 (MOI = 1) in the presence of either U73122 or D-glucose alone, or in combination, at the designated concentrations, at 37°C for 30 min. After extensive washes, genomic DNA was extracted, and the viral genome bound to the cells was quantitatively evaluated using qPCR. The data shown are the means of three independent experiments with error bars representing the SDs. The significance was evaluated by Student’s t -test (*, P < 0.05; **, P < 0.01).

Article Snippet: Phospholipase C (PLC) inhibitor U73122 (cat# HY-13419) and GLUT1-specific inhibitor BAY-876 (cat# HY-100017) were ordered from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Infection