Journal: Journal of Functional Biomaterials

Article Title: Skin Vaccination with Ebola Virus Glycoprotein Using a Polyphosphazene-Based Microneedle Patch Protects Mice against Lethal Challenge

doi: 10.3390/jfb14010016

Figure Lengend Snippet: Characterization of antigen-PCPP complex. ( A ) Representative AF4 fractograms of antigen, PCPP, and antigen-PCPP complex. ( B ) Representative DLS profiles of PCPP and antigen-PCPP complex (0.05 mg/mL antigen and 0.25 mg/mL PCPP).

Article Snippet: Dynamic light scattering (DLS) characterization of PCPP formulations was carried out using Malvern Zetasizer Nano series instrument (Malvern Instruments, Worcestershire, UK).

Techniques:

Journal: ACS applied bio materials

Article Title: Supramolecular assembly of Toll-like receptor 7/8 agonist into multimeric water-soluble constructs enables superior immune stimulation in vitro and in vivo

doi: 10.1021/acsabm.0c00189

Figure Lengend Snippet: Schematic presentation of interactions of PCPP and RSQD with formation of multimeric supramolecular assembly PCPP-R.

Article Snippet: Aqueous solutions of PCPP and RSQD (Sigma Aldrich, St. Louis, MO) were mixed using microfluidics system with staggered herringbone mixer, SHM (Microfluidic ChipShop, Jena, Germany).

Techniques:

Journal: ACS applied bio materials

Article Title: Supramolecular assembly of Toll-like receptor 7/8 agonist into multimeric water-soluble constructs enables superior immune stimulation in vitro and in vivo

doi: 10.1021/acsabm.0c00189

Figure Lengend Snippet: Physico-chemical characterization of PCPP-RSQD complexes: (A) Turbidimetric titration of PCPP with RSQD (0.5 mg/mL PCPP, 0–1 mg/mL RSQD, PBS, pH 7.4, SD); (B) z-average hydrodynamic diameter of PCPP – RSQD system (0.5 mg/mL PCPP, 0–1 mg/mL RSQD, PBS, pH 7.4, SD); (C) fluorescence spectroscopy spectra of RSQD in the presence of PCPP and (D) maximum fluorescence peak intensity of RSQD vs. PCPP concentration (Ex/Em=250/350 nm; 0.025 mg/mL RSQD, 0–0.5 mg/mL PCPP, PBS, pH 7.4); (E) PCPP – RSQD side group with 1H NMR peak shifts assigned (RSQD:COO−(PCPP)=1:1 (mol/equiv), 1M KOH in D2O); (F) AF4 fractograms of PCPP, RSQD (no signal) and PCPP-RSQD (0.25 mg/mL PCPP; 0.125 mg/mL RSQD, PBS, pH 7.4).

Article Snippet: Aqueous solutions of PCPP and RSQD (Sigma Aldrich, St. Louis, MO) were mixed using microfluidics system with staggered herringbone mixer, SHM (Microfluidic ChipShop, Jena, Germany).

Techniques: Titration, Fluorescence, Spectroscopy, Concentration Assay

Journal: ACS applied bio materials

Article Title: Supramolecular assembly of Toll-like receptor 7/8 agonist into multimeric water-soluble constructs enables superior immune stimulation in vitro and in vivo

doi: 10.1021/acsabm.0c00189

Figure Lengend Snippet: In vitro assessment of PCPP-R: (A) Molecular composition of PCPP-R (number of RSQD counterions per PCPP chain) vs incubation time (Franz diffusion cell, 0.125 mg/mL RSQD, 0.25 mg/mL PCPP, PBS, pH 7.4, SD); (B) hemolytic activity of RSQD and PCPP-R (human erythrocytes, 0.5 mg/mL PCPP, no activity detected for PCPP); (C) In vitro stimulation of macrophages by PCPP-R and RSQD (0.01 – 1 μg/mL RSQD, PCPP-R (PCPP:RSQD=20:1 (w/w)), RAW Blue cell line; two-tailed Students t-test; R2 > 0.96, n=3; no response detected for PCPP); (D) AF4 fractograms of PCPP-R – E2, E2, PCPP, PCPP-E2, and PCPP-R (0.125 mg/mL E2, 0.125 mg/mL PCPP, 0.062 mg/mL RSQD, PBS, pH 7.4).

Article Snippet: Aqueous solutions of PCPP and RSQD (Sigma Aldrich, St. Louis, MO) were mixed using microfluidics system with staggered herringbone mixer, SHM (Microfluidic ChipShop, Jena, Germany).

Techniques: In Vitro, Incubation, Diffusion-based Assay, Activity Assay, Two Tailed Test