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A , Representative SRRF confocal images of improved ASAP (axonally-targeted ASAP: axASAP) at the soma/dendrite (left) and at a distal axon of a PC (right). B , Fluorescent intensity at soma, dendrite, axon, and presynaptic bouton of original <t>ASAP1</t> or axASAP. N = 41 (ASAP1) and 39 cells (axASAP1). ***, p < 0.005; *, p < 0.05.
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A , Representative SRRF confocal images of improved ASAP (axonally-targeted ASAP: axASAP) at the soma/dendrite (left) and at a distal axon of a PC (right). B , Fluorescent intensity at soma, dendrite, axon, and presynaptic bouton of original <t>ASAP1</t> or axASAP. N = 41 (ASAP1) and 39 cells (axASAP1). ***, p < 0.005; *, p < 0.05.
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A , Representative SRRF confocal images of improved ASAP (axonally-targeted ASAP: axASAP) at the soma/dendrite (left) and at a distal axon of a PC (right). B , Fluorescent intensity at soma, dendrite, axon, and presynaptic bouton of original <t>ASAP1</t> or axASAP. N = 41 (ASAP1) and 39 cells (axASAP1). ***, p < 0.005; *, p < 0.05.
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A , Representative SRRF confocal images of improved ASAP (axonally-targeted ASAP: axASAP) at the soma/dendrite (left) and at a distal axon of a PC (right). B , Fluorescent intensity at soma, dendrite, axon, and presynaptic bouton of original <t>ASAP1</t> or axASAP. N = 41 (ASAP1) and 39 cells (axASAP1). ***, p < 0.005; *, p < 0.05.
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A , Representative SRRF confocal images of improved ASAP (axonally-targeted ASAP: axASAP) at the soma/dendrite (left) and at a distal axon of a PC (right). B , Fluorescent intensity at soma, dendrite, axon, and presynaptic bouton of original <t>ASAP1</t> or axASAP. N = 41 (ASAP1) and 39 cells (axASAP1). ***, p < 0.005; *, p < 0.05.
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A , Representative SRRF confocal images of improved ASAP (axonally-targeted ASAP: axASAP) at the soma/dendrite (left) and at a distal axon of a PC (right). B , Fluorescent intensity at soma, dendrite, axon, and presynaptic bouton of original <t>ASAP1</t> or axASAP. N = 41 (ASAP1) and 39 cells (axASAP1). ***, p < 0.005; *, p < 0.05.
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A , Representative SRRF confocal images of improved ASAP (axonally-targeted ASAP: axASAP) at the soma/dendrite (left) and at a distal axon of a PC (right). B , Fluorescent intensity at soma, dendrite, axon, and presynaptic bouton of original <t>ASAP1</t> or axASAP. N = 41 (ASAP1) and 39 cells (axASAP1). ***, p < 0.005; *, p < 0.05.
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A , Representative SRRF confocal images of improved ASAP (axonally-targeted ASAP: axASAP) at the soma/dendrite (left) and at a distal axon of a PC (right). B , Fluorescent intensity at soma, dendrite, axon, and presynaptic bouton of original <t>ASAP1</t> or axASAP. N = 41 (ASAP1) and 39 cells (axASAP1). ***, p < 0.005; *, p < 0.05.
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A , Representative SRRF confocal images of improved ASAP (axonally-targeted ASAP: axASAP) at the soma/dendrite (left) and at a distal axon of a PC (right). B , Fluorescent intensity at soma, dendrite, axon, and presynaptic bouton of original <t>ASAP1</t> or axASAP. N = 41 (ASAP1) and 39 cells (axASAP1). ***, p < 0.005; *, p < 0.05.
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Image Search Results


A , Representative SRRF confocal images of improved ASAP (axonally-targeted ASAP: axASAP) at the soma/dendrite (left) and at a distal axon of a PC (right). B , Fluorescent intensity at soma, dendrite, axon, and presynaptic bouton of original ASAP1 or axASAP. N = 41 (ASAP1) and 39 cells (axASAP1). ***, p < 0.005; *, p < 0.05.

Journal: bioRxiv

Article Title: Long-Term Reconfiguration of Dynamic Polarization and Action Potential Initiation in Purkinje Cell Axons

doi: 10.64898/2026.10.12.758508

Figure Lengend Snippet: A , Representative SRRF confocal images of improved ASAP (axonally-targeted ASAP: axASAP) at the soma/dendrite (left) and at a distal axon of a PC (right). B , Fluorescent intensity at soma, dendrite, axon, and presynaptic bouton of original ASAP1 or axASAP. N = 41 (ASAP1) and 39 cells (axASAP1). ***, p < 0.005; *, p < 0.05.

Article Snippet: The coding DNA sequence for ASAP1 was obtained from Addgene (#52519), and some modifications for higher voltage sensitivity (D145D, L146G, S147T, N149R, S150G, and H151D) established in an improved version ASAP3 ( ) were introduced by PCR-mediated mutations (noted here as ASAP3β; ).

Techniques: Confocal

A , Representative image of a HEK293T cell expressing axASAP3. B , Voltage-step protocol (holding potential: −70 mV; steps to −100 ∼ +50 mV) and corresponding optical fluorescence responses (ΔF/F) of ASAP1, axASAP1, and axASAP3. C , D , Normalized fluorescence change time course during 100-mV depolarization (C) and repolarization (D), comparing original ASAP1, axASAP1, and axASAP3. Fast (Ƭ fast ) and slow (Ƭ slow ) time constants and the relative amplitude of the fast component (% fast) are indicated for each probe. E , Simultaneous fluorescent voltage imaging (red, 1.5 kHz) and whole-cell current-clamp electrical (black) recordings of a single AP in the PC soma expressing axASAP1 (left) or original ASAP1 (right), illustrating the fast fluorescence recovery of axASAP1 providing better fluorescent tracking of APs.

Journal: bioRxiv

Article Title: Long-Term Reconfiguration of Dynamic Polarization and Action Potential Initiation in Purkinje Cell Axons

doi: 10.64898/2026.10.12.758508

Figure Lengend Snippet: A , Representative image of a HEK293T cell expressing axASAP3. B , Voltage-step protocol (holding potential: −70 mV; steps to −100 ∼ +50 mV) and corresponding optical fluorescence responses (ΔF/F) of ASAP1, axASAP1, and axASAP3. C , D , Normalized fluorescence change time course during 100-mV depolarization (C) and repolarization (D), comparing original ASAP1, axASAP1, and axASAP3. Fast (Ƭ fast ) and slow (Ƭ slow ) time constants and the relative amplitude of the fast component (% fast) are indicated for each probe. E , Simultaneous fluorescent voltage imaging (red, 1.5 kHz) and whole-cell current-clamp electrical (black) recordings of a single AP in the PC soma expressing axASAP1 (left) or original ASAP1 (right), illustrating the fast fluorescence recovery of axASAP1 providing better fluorescent tracking of APs.

Article Snippet: The coding DNA sequence for ASAP1 was obtained from Addgene (#52519), and some modifications for higher voltage sensitivity (D145D, L146G, S147T, N149R, S150G, and H151D) established in an improved version ASAP3 ( ) were introduced by PCR-mediated mutations (noted here as ASAP3β; ).

Techniques: Expressing, Fluorescence, Imaging