Journal: ACS Omega

Article Title: Low-Level Laser Mitigates Cisplatin-Induced Oxidative Stress and Apoptosis via AMPK-Mediated Autophagy in PC12 Cells

doi: 10.1021/acsomega.5c12039

Figure Lengend Snippet: Low-level laser (LLL) exposure improves cisplatin-induced cytotoxicity and apoptosis in PC12 cells. Cisplatin-induced reduction in cell viability (A) and increase in cytotoxicity (B) were markedly attenuated by LLL treatment at 5 J/cm 2 . (C) Apoptosis triggered by cisplatin was reduced by LLL irradiation, as determined by TUNEL assay with flow cytometry. Results are expressed as means ± SD of three independent experiments. * Indicates p < 0.05 compared with the control group.

Article Snippet: Rat pheochromocytoma PC12 cells were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM supplemented with 10% heat-inactivated horse serum, 5% FBS, 1% PS, and 1% l -glutamine.

Techniques: Irradiation, TUNEL Assay, Flow Cytometry, Control

Journal: ACS Omega

Article Title: Low-Level Laser Mitigates Cisplatin-Induced Oxidative Stress and Apoptosis via AMPK-Mediated Autophagy in PC12 Cells

doi: 10.1021/acsomega.5c12039

Figure Lengend Snippet: LLL enhances the cisplatin-induced autophagy in an AMPK-dependent manner. Representative Western blot images (A) and corresponding densitometric bar graphs (B, C, D) of Beclin-1, LC3-I/II, and p62 in PC12 cells exposed to cisplatin, either alone, in combination with 5 J/cm 2 LLL, or under AMPK silencing conditions, are presented. Results are expressed as means ± SD of three independent experiments. * Indicates p < 0.05 compared with the control group. # Indicates p < 0.05 compared to the cisplatin group. & Indicates p < 0.05 compared to the cisplatin + LLL group.

Article Snippet: Rat pheochromocytoma PC12 cells were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM supplemented with 10% heat-inactivated horse serum, 5% FBS, 1% PS, and 1% l -glutamine.

Techniques: Western Blot, Control

Journal: ACS Omega

Article Title: Low-Level Laser Mitigates Cisplatin-Induced Oxidative Stress and Apoptosis via AMPK-Mediated Autophagy in PC12 Cells

doi: 10.1021/acsomega.5c12039

Figure Lengend Snippet: LLL reduces oxidative stress and changes in mitochondrial membrane potential evoked by cisplatin through AMPK-mediated autophagy. Representative measurements of (A) malondialdehyde (MDA) and (B) mitochondrial superoxide levels in PC12 cells treated with cisplatin, and further subjected to LLL, AMPK knockdown, or rapamycin-induced autophagy. Results were expressed as means ± SD of three independent experiments. * Indicates p < 0.05 compared with the control group. # Indicates p < 0.05 compared to the cisplatin group. & Indicates p < 0.05 compared to the cisplatin + LLL group. Mitochondrial membrane potential was assessed using JC-1 staining. Fluorescence intensities in the FL-1 (green) and FL-2 (red) channels were measured by flow cytometry and quantified, with the results presented as a bar graph (C). Experimental conditions included treatment with chloroquine (an autophagy inhibitor) and MitoTEMPO (a mitochondria-targeted antioxidant), either alone or in combination with cisplatin and/or LLL.

Article Snippet: Rat pheochromocytoma PC12 cells were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM supplemented with 10% heat-inactivated horse serum, 5% FBS, 1% PS, and 1% l -glutamine.

Techniques: Membrane, Knockdown, Control, Staining, Fluorescence, Flow Cytometry

Journal: ACS Omega

Article Title: Low-Level Laser Mitigates Cisplatin-Induced Oxidative Stress and Apoptosis via AMPK-Mediated Autophagy in PC12 Cells

doi: 10.1021/acsomega.5c12039

Figure Lengend Snippet: LLL inhibits cisplatin-induced apoptosis through the activation of AMPK. Representative Western blot images (A) and corresponding densitometric bar graphs (B-E) of p53, Bax, Bcl-2, and cytosolic cytochrome c in PC12 cells exposed to cisplatin, either alone, in combination with 5 J/cm 2 LLL, or under AMPK silencing conditions, were presented. Results are expressed as means ± SD of three independent experiments. * Indicates p < 0.05 compared with the control group. # Indicates p < 0.05 compared to the cisplatin group. & Indicates p < 0.05 compared to the cisplatin + LLL group.

Article Snippet: Rat pheochromocytoma PC12 cells were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM supplemented with 10% heat-inactivated horse serum, 5% FBS, 1% PS, and 1% l -glutamine.

Techniques: Activation Assay, Western Blot, Control