Journal: bioRxiv

Article Title: Sensory-motor integration in a nonspiking interneuron contributes to active sensor control in Drosophila

doi: 10.64898/2026.04.16.718965

Figure Lengend Snippet: A) Schematic depicting JONs projecting through the antennal nerve and synapsing onto APN2 cells (gold dashed box) in the ipsilateral hemisphere of the central brain. Inset shows expression pattern of the genetic driver line labeling APN2 ( 24C06-GAL4) . Scale bar is 20 µm. B) During whole cell patch clamp recordings, antennal movements were recorded by a lateral camera and flight activity was monitored using an optical wingbeat detector. C) Example video frame with 2 tracked points and the relative direction of antenna angle deflections. Deflections down towards the head are represented as negative values, and deflections up and away from the head as positive values. D) Example single-trial traces showing antennal and APN2 activity during quiescence (left) and flight (right). Wing movement was detected using an infrared light sensor. E) Each dot is the average response of one APN2 cell. Black bars indicate the average across APN2 cells for each condition, with respective SEM error bars. Grey dashed lines pair measurements from the same cell during flight and quiescence. Across flies, the average APN2 membrane potential is reduced during bouts of flight compared to quiescence, both when the antennae are free (paired t-test; p = 0.037) and when the antennae are glued (Wilcoxon Signed-Rank Test; p = 0.014).

Article Snippet: We used 6-11 MΩ thick-walled glass pipettes (Item #1B150F-3, World Precision Instruments) for whole cell patch clamp recordings, which we pulled using a Sutter P-1000 puller.

Techniques: Expressing, Labeling, Patch Clamp, Activity Assay, Membrane

Journal: iScience

Article Title: Ether phospholipids modulate somatosensory responses by tuning multiple receptor functions in Drosophila

doi: 10.1016/j.isci.2026.115209

Figure Lengend Snippet: ePLs modify dPIEZO activity in Drosophila cells (A–C) Representative Fura-2 imaging data for dPIEZO in control S2R+ cells and cells supplemented with 100 μM 18-AG. A, Typical ratiometric images before (Basal, at 60 s) and after the application of 10 μM Yoda1 (Yoda1, at 360 s) or 5 μM ionomycin (Ionomycin, at 540 s) in dPIEZO expressing cells (left) and dPIEZO expressing cells supplemented with 100 μM 18-AG (right). B, C, Representative Ca 2+ level traces in dPIEZO expressing cells (B), and 18-AG supplemented dPIEZO expressing cells (C). Red traces indicate Yoda1 responding cells (Δratio > 2). (D, E) Proportion of cells responding to Yoda1 (Δratio > 2) (D) and maximum Δratio response to Yoda1 normalized by the ionomycin response (E) in mock-transfected cells (control; n = 10, 18-AG; n = 10) and dPIEZO expressing cells (control, n = 10, 18-AG, n = 10). Each point represents a biological replicate; 25–40 cells were analyzed in each assay. Data are presented as mean ± SEM. ∗ p < 0.05 and ∗∗ p < 0.01; Tukey’s test. (F, G) Representative traces of patch-clamp recordings of mechanical stimuli-evoked dPIEZO activation without (F) or with (G) 100 μM 18-AG. (H) Quantification of the peak current density. Data are presented as mean ± SEM. ∗∗ p < 0.01; Mann-Whitney U test. (I) Proportion of responding cells (current size > 5 pA).

Article Snippet: Thermistor for temperature measurement in patch-clamp , Warner instruments , TA-29.

Techniques: Activity Assay, Imaging, Control, Expressing, Transfection, Patch Clamp, Activation Assay, MANN-WHITNEY

Journal: iScience

Article Title: Ether phospholipids modulate somatosensory responses by tuning multiple receptor functions in Drosophila

doi: 10.1016/j.isci.2026.115209

Figure Lengend Snippet: ePLs modulate the temperature threshold for dTRPA1-A activation (A, B, E, F) Whole-cell patch-clamp recording of dTRPA1-A activation with temperature stimulation in dTRPA1-A expressing S2R+ cells (A), dTRPA1 expressing cells supplemented with 100 μM 18-AG (B), dAGPS-expressing S2R+ cells (E), and dAGPS-expressing S2R+ cells supplemented with 100 μM 18-OH (18-OH) (F) Left, representative traces of recordings; right, Arrhenius plots from the traces in the left panels. Temperature thresholds were determined as the crossing point of two fitted lines. (C, D, G, H) Quantification of the temperature threshold (C, G) and peak current densities from heat (D, H) in dTRPA1 expressing cells supplemented with solvent (control) or 18-AG (C, D), and dAGPS-expressing cells (control) or dAGPS-expressing cells supplemented with 100 μM 18-OH (18-OH) (G, H). Each point represents a biological replicate. The number of replicates (n) is shown in the panels. Data are presented as mean ± SEM. ∗ p < 0.05 and ∗∗ p < 0.01; Student’s t test. See also .

Article Snippet: Thermistor for temperature measurement in patch-clamp , Warner instruments , TA-29.

Techniques: Activation Assay, Patch Clamp, Expressing, Solvent, Control