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rabbit anti p2y2  (Alomone Labs)
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Alomone Labs rabbit anti p2y2
Rabbit Anti P2y2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p2y2+receptor/Anti-P2Y2+Receptor+Antibody/10__1016_slash_j__ghres__2026__100021-55-3-6
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p2y2  (Alomone Labs)
95
Alomone Labs p2y2
Protein changes and transcriptional expression of <t>P2Y2</t> following injury. (A) For a naïve/non‐injured fish, 2 mm of thoracic spinal cord was collected. (B) For injured fish, 2 mm of spinal cord was collected containing the injury site, as well as 2 mm immediately adjacent on each side (Ro = Rostral, Ca = Caudal). (C) Representative western blot and corresponding total protein showing naïve zebrafish (Zf) spinal cord tissue, de‐glycosylated (Dg) naïve zebrafish spinal cord, mouse brain tissue as a positive control (+), and naïve zebrafish spinal cord tissue pre‐absorbed with a blocking peptide as a negative control (−). (D, F, H) Representative western blot and corresponding total protein at 7 dpi, 14 dpi, and 21 dpi, respectively. (E) Quantitative analysis showing significant upregulation of the 50 kDa isoform within the rostral ( p < 0.0001), injury ( p = 0.0119), and caudal site ( p = 0.0002) at 7 dpi when normalized to naïve tissue. (G) Quantitative analysis of P2Y2 showing basal levels of expression at 14 dpi when normalized to naïve tissue. (I) Quantitative analysis showing significant downregulation of the 25 kDa isoform within the rostral ( p = 0.0073), injury ( p = 0.0015), and caudal site ( p = 0.0002) at 21 dpi when normalized to naïve tissue. (J) Heat map showing RNA‐seq analysis of P2Y2 gene transcripts compared to highly up‐ and down‐regulated genes in rostral and caudal tissue at 7 and 14 dpi. (K, L) Volcano plot of the zebrafish transcriptome at 7 dpi with P2Y2 transcripts and highly up‐ and down‐regulated genes labeled in rostral and caudal tissue, respectively. Total protein used as loading control. One‐way ANOVA with Dunnett's post hoc test was used for western blot analysis. Data presented as means ± SEM. *Significant differences: p ≤ 0.05. **Significant differences: p ≤ 0.005. ***significant differences: p ≤ 0.001. For RNA‐seq analysis, calculated adjusted p ‐values < 0.05 and a log2(fold change) < −1 or > 1 were considered differentially expressed. **** Significant differences: p ≤ 0.0001.
P2y2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p2y2+receptor/Anti-P2Y2+Receptor+Antibody/pmc13545662-109-27-31
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blocking peptide  (Alomone Labs)
94
Alomone Labs blocking peptide
Protein changes and transcriptional expression of <t>P2Y2</t> following injury. (A) For a naïve/non‐injured fish, 2 mm of thoracic spinal cord was collected. (B) For injured fish, 2 mm of spinal cord was collected containing the injury site, as well as 2 mm immediately adjacent on each side (Ro = Rostral, Ca = Caudal). (C) Representative western blot and corresponding total protein showing naïve zebrafish (Zf) spinal cord tissue, de‐glycosylated (Dg) naïve zebrafish spinal cord, mouse brain tissue as a positive control (+), and naïve zebrafish spinal cord tissue pre‐absorbed with a blocking peptide as a negative control (−). (D, F, H) Representative western blot and corresponding total protein at 7 dpi, 14 dpi, and 21 dpi, respectively. (E) Quantitative analysis showing significant upregulation of the 50 kDa isoform within the rostral ( p < 0.0001), injury ( p = 0.0119), and caudal site ( p = 0.0002) at 7 dpi when normalized to naïve tissue. (G) Quantitative analysis of P2Y2 showing basal levels of expression at 14 dpi when normalized to naïve tissue. (I) Quantitative analysis showing significant downregulation of the 25 kDa isoform within the rostral ( p = 0.0073), injury ( p = 0.0015), and caudal site ( p = 0.0002) at 21 dpi when normalized to naïve tissue. (J) Heat map showing RNA‐seq analysis of P2Y2 gene transcripts compared to highly up‐ and down‐regulated genes in rostral and caudal tissue at 7 and 14 dpi. (K, L) Volcano plot of the zebrafish transcriptome at 7 dpi with P2Y2 transcripts and highly up‐ and down‐regulated genes labeled in rostral and caudal tissue, respectively. Total protein used as loading control. One‐way ANOVA with Dunnett's post hoc test was used for western blot analysis. Data presented as means ± SEM. *Significant differences: p ≤ 0.05. **Significant differences: p ≤ 0.005. ***significant differences: p ≤ 0.001. For RNA‐seq analysis, calculated adjusted p ‐values < 0.05 and a log2(fold change) < −1 or > 1 were considered differentially expressed. **** Significant differences: p ≤ 0.0001.
Blocking Peptide, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p2y2+receptor/P2Y2+Receptor+(extracellular)+Blocking+Peptide/pmc13545662-89-7-19
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p2ry2 antibody  (Alomone Labs)
94
Alomone Labs p2ry2 antibody
Protein changes and transcriptional expression of <t>P2Y2</t> following injury. (A) For a naïve/non‐injured fish, 2 mm of thoracic spinal cord was collected. (B) For injured fish, 2 mm of spinal cord was collected containing the injury site, as well as 2 mm immediately adjacent on each side (Ro = Rostral, Ca = Caudal). (C) Representative western blot and corresponding total protein showing naïve zebrafish (Zf) spinal cord tissue, de‐glycosylated (Dg) naïve zebrafish spinal cord, mouse brain tissue as a positive control (+), and naïve zebrafish spinal cord tissue pre‐absorbed with a blocking peptide as a negative control (−). (D, F, H) Representative western blot and corresponding total protein at 7 dpi, 14 dpi, and 21 dpi, respectively. (E) Quantitative analysis showing significant upregulation of the 50 kDa isoform within the rostral ( p < 0.0001), injury ( p = 0.0119), and caudal site ( p = 0.0002) at 7 dpi when normalized to naïve tissue. (G) Quantitative analysis of P2Y2 showing basal levels of expression at 14 dpi when normalized to naïve tissue. (I) Quantitative analysis showing significant downregulation of the 25 kDa isoform within the rostral ( p = 0.0073), injury ( p = 0.0015), and caudal site ( p = 0.0002) at 21 dpi when normalized to naïve tissue. (J) Heat map showing RNA‐seq analysis of P2Y2 gene transcripts compared to highly up‐ and down‐regulated genes in rostral and caudal tissue at 7 and 14 dpi. (K, L) Volcano plot of the zebrafish transcriptome at 7 dpi with P2Y2 transcripts and highly up‐ and down‐regulated genes labeled in rostral and caudal tissue, respectively. Total protein used as loading control. One‐way ANOVA with Dunnett's post hoc test was used for western blot analysis. Data presented as means ± SEM. *Significant differences: p ≤ 0.05. **Significant differences: p ≤ 0.005. ***significant differences: p ≤ 0.001. For RNA‐seq analysis, calculated adjusted p ‐values < 0.05 and a log2(fold change) < −1 or > 1 were considered differentially expressed. **** Significant differences: p ≤ 0.0001.
P2ry2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p2y2+receptor/Anti-P2Y2+Receptor+(extracellular)-FITC+Antibody/pm41887391-341-37-40
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p2ry2  (Alomone Labs)
95
Alomone Labs p2ry2
Protein changes and transcriptional expression of <t>P2Y2</t> following injury. (A) For a naïve/non‐injured fish, 2 mm of thoracic spinal cord was collected. (B) For injured fish, 2 mm of spinal cord was collected containing the injury site, as well as 2 mm immediately adjacent on each side (Ro = Rostral, Ca = Caudal). (C) Representative western blot and corresponding total protein showing naïve zebrafish (Zf) spinal cord tissue, de‐glycosylated (Dg) naïve zebrafish spinal cord, mouse brain tissue as a positive control (+), and naïve zebrafish spinal cord tissue pre‐absorbed with a blocking peptide as a negative control (−). (D, F, H) Representative western blot and corresponding total protein at 7 dpi, 14 dpi, and 21 dpi, respectively. (E) Quantitative analysis showing significant upregulation of the 50 kDa isoform within the rostral ( p < 0.0001), injury ( p = 0.0119), and caudal site ( p = 0.0002) at 7 dpi when normalized to naïve tissue. (G) Quantitative analysis of P2Y2 showing basal levels of expression at 14 dpi when normalized to naïve tissue. (I) Quantitative analysis showing significant downregulation of the 25 kDa isoform within the rostral ( p = 0.0073), injury ( p = 0.0015), and caudal site ( p = 0.0002) at 21 dpi when normalized to naïve tissue. (J) Heat map showing RNA‐seq analysis of P2Y2 gene transcripts compared to highly up‐ and down‐regulated genes in rostral and caudal tissue at 7 and 14 dpi. (K, L) Volcano plot of the zebrafish transcriptome at 7 dpi with P2Y2 transcripts and highly up‐ and down‐regulated genes labeled in rostral and caudal tissue, respectively. Total protein used as loading control. One‐way ANOVA with Dunnett's post hoc test was used for western blot analysis. Data presented as means ± SEM. *Significant differences: p ≤ 0.05. **Significant differences: p ≤ 0.005. ***significant differences: p ≤ 0.001. For RNA‐seq analysis, calculated adjusted p ‐values < 0.05 and a log2(fold change) < −1 or > 1 were considered differentially expressed. **** Significant differences: p ≤ 0.0001.
P2ry2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p2y2+receptor/Anti-P2Y2+Receptor+Antibody/pm41887391-368-8-9
Average 95 stars, based on 1 article reviews
p2ry2 - by Bioz Stars, 2026-10
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Protein changes and transcriptional expression of P2Y2 following injury. (A) For a naïve/non‐injured fish, 2 mm of thoracic spinal cord was collected. (B) For injured fish, 2 mm of spinal cord was collected containing the injury site, as well as 2 mm immediately adjacent on each side (Ro = Rostral, Ca = Caudal). (C) Representative western blot and corresponding total protein showing naïve zebrafish (Zf) spinal cord tissue, de‐glycosylated (Dg) naïve zebrafish spinal cord, mouse brain tissue as a positive control (+), and naïve zebrafish spinal cord tissue pre‐absorbed with a blocking peptide as a negative control (−). (D, F, H) Representative western blot and corresponding total protein at 7 dpi, 14 dpi, and 21 dpi, respectively. (E) Quantitative analysis showing significant upregulation of the 50 kDa isoform within the rostral ( p < 0.0001), injury ( p = 0.0119), and caudal site ( p = 0.0002) at 7 dpi when normalized to naïve tissue. (G) Quantitative analysis of P2Y2 showing basal levels of expression at 14 dpi when normalized to naïve tissue. (I) Quantitative analysis showing significant downregulation of the 25 kDa isoform within the rostral ( p = 0.0073), injury ( p = 0.0015), and caudal site ( p = 0.0002) at 21 dpi when normalized to naïve tissue. (J) Heat map showing RNA‐seq analysis of P2Y2 gene transcripts compared to highly up‐ and down‐regulated genes in rostral and caudal tissue at 7 and 14 dpi. (K, L) Volcano plot of the zebrafish transcriptome at 7 dpi with P2Y2 transcripts and highly up‐ and down‐regulated genes labeled in rostral and caudal tissue, respectively. Total protein used as loading control. One‐way ANOVA with Dunnett's post hoc test was used for western blot analysis. Data presented as means ± SEM. *Significant differences: p ≤ 0.05. **Significant differences: p ≤ 0.005. ***significant differences: p ≤ 0.001. For RNA‐seq analysis, calculated adjusted p ‐values < 0.05 and a log2(fold change) < −1 or > 1 were considered differentially expressed. **** Significant differences: p ≤ 0.0001.

Journal: The FASEB Journal

Article Title: Purinergic Receptor P2Y2 Regulates Neurogenesis and Functional Recovery Following Spinal Cord Injury in Zebrafish

doi: 10.1096/fj.202504890RR

Figure Lengend Snippet: Protein changes and transcriptional expression of P2Y2 following injury. (A) For a naïve/non‐injured fish, 2 mm of thoracic spinal cord was collected. (B) For injured fish, 2 mm of spinal cord was collected containing the injury site, as well as 2 mm immediately adjacent on each side (Ro = Rostral, Ca = Caudal). (C) Representative western blot and corresponding total protein showing naïve zebrafish (Zf) spinal cord tissue, de‐glycosylated (Dg) naïve zebrafish spinal cord, mouse brain tissue as a positive control (+), and naïve zebrafish spinal cord tissue pre‐absorbed with a blocking peptide as a negative control (−). (D, F, H) Representative western blot and corresponding total protein at 7 dpi, 14 dpi, and 21 dpi, respectively. (E) Quantitative analysis showing significant upregulation of the 50 kDa isoform within the rostral ( p < 0.0001), injury ( p = 0.0119), and caudal site ( p = 0.0002) at 7 dpi when normalized to naïve tissue. (G) Quantitative analysis of P2Y2 showing basal levels of expression at 14 dpi when normalized to naïve tissue. (I) Quantitative analysis showing significant downregulation of the 25 kDa isoform within the rostral ( p = 0.0073), injury ( p = 0.0015), and caudal site ( p = 0.0002) at 21 dpi when normalized to naïve tissue. (J) Heat map showing RNA‐seq analysis of P2Y2 gene transcripts compared to highly up‐ and down‐regulated genes in rostral and caudal tissue at 7 and 14 dpi. (K, L) Volcano plot of the zebrafish transcriptome at 7 dpi with P2Y2 transcripts and highly up‐ and down‐regulated genes labeled in rostral and caudal tissue, respectively. Total protein used as loading control. One‐way ANOVA with Dunnett's post hoc test was used for western blot analysis. Data presented as means ± SEM. *Significant differences: p ≤ 0.05. **Significant differences: p ≤ 0.005. ***significant differences: p ≤ 0.001. For RNA‐seq analysis, calculated adjusted p ‐values < 0.05 and a log2(fold change) < −1 or > 1 were considered differentially expressed. **** Significant differences: p ≤ 0.0001.

Article Snippet: All tissue sections were then incubated at room temperature overnight in a 0.1% Triton X‐100/0.01 M PBS solution containing at least one of the following primary antibodies: P2Y2 (Rabbit polyclonal, 1:200, Almone labs: APR010AN0802, RRID: AB_2040078), GFAP (Chicken polyclonal, 1:2000, ThermoFisher Scientific: PA1‐10004, RRID: AB_1074620), PCNA (Mouse monoclonal, 1:1000, Abcam: ab29, RRID: AB_303394), or HuC/D (Mouse Monoclonal, 1:20 000, ThermoFisher Scientific: A‐21271, RRID: AB_221448).

Techniques: Expressing, Western Blot, Tissue, Positive Control, Blocking Assay, Negative Control, Analysis, RNA Sequencing, Labeling, Control

Behavioral analysis following P2Y2 inhibition at 14 dpi and 21 dpi. (A) Schematic representation of treatment dosages, timeline, and behavioral collection. (B) Fish spent 5 min being recorded unstimulated and uninterrupted in an “open field” paradigm. (C) Fish spent 5 min in the open field chamber with a single “tail touch” stimulation every 30 s. (D) Fish were recorded swimming in a swim tunnel against increasing flow speeds until exhaustion (10 s against the back of the swim tunnel). (E) Swim tunnel paradigm indicating swim tunnel water flow speeds (body length s −1 ; 1 body length = 2 cm) over time (min). The paradigm continues until the fish is exhausted, or 60 min have passed. (F, J) Total distance traveled during the open field paradigm (B) represented as % values of each individual's naïve trial. (G, K) Average degree changes in mean body angle during the tail touch paradigm (C) represented as a % value of each individual's naïve trial. (H, L) Average rostral compensation (tail: Nose movement ratio) during the tail touch paradigm (C) represented as a % value of each individual's naïve trial. (I, M) Time to exhaustion in the swim tunnel paradigm (D, E) represented as a % value of each individual's naïve trial. At 14 dpi (I) the AR‐C group showed a significantly decreased time to exhaustion ( n = 12, p = 0.0427) when compared to the saline group ( n = 10). Unpaired t test (G, H, J–L) and lognormal unpaired t test: Welch variant (F, I, M) used for analysis. Data presented as means ± SEM. *Significant differences: p ≤ 0.05.

Journal: The FASEB Journal

Article Title: Purinergic Receptor P2Y2 Regulates Neurogenesis and Functional Recovery Following Spinal Cord Injury in Zebrafish

doi: 10.1096/fj.202504890RR

Figure Lengend Snippet: Behavioral analysis following P2Y2 inhibition at 14 dpi and 21 dpi. (A) Schematic representation of treatment dosages, timeline, and behavioral collection. (B) Fish spent 5 min being recorded unstimulated and uninterrupted in an “open field” paradigm. (C) Fish spent 5 min in the open field chamber with a single “tail touch” stimulation every 30 s. (D) Fish were recorded swimming in a swim tunnel against increasing flow speeds until exhaustion (10 s against the back of the swim tunnel). (E) Swim tunnel paradigm indicating swim tunnel water flow speeds (body length s −1 ; 1 body length = 2 cm) over time (min). The paradigm continues until the fish is exhausted, or 60 min have passed. (F, J) Total distance traveled during the open field paradigm (B) represented as % values of each individual's naïve trial. (G, K) Average degree changes in mean body angle during the tail touch paradigm (C) represented as a % value of each individual's naïve trial. (H, L) Average rostral compensation (tail: Nose movement ratio) during the tail touch paradigm (C) represented as a % value of each individual's naïve trial. (I, M) Time to exhaustion in the swim tunnel paradigm (D, E) represented as a % value of each individual's naïve trial. At 14 dpi (I) the AR‐C group showed a significantly decreased time to exhaustion ( n = 12, p = 0.0427) when compared to the saline group ( n = 10). Unpaired t test (G, H, J–L) and lognormal unpaired t test: Welch variant (F, I, M) used for analysis. Data presented as means ± SEM. *Significant differences: p ≤ 0.05.

Article Snippet: All tissue sections were then incubated at room temperature overnight in a 0.1% Triton X‐100/0.01 M PBS solution containing at least one of the following primary antibodies: P2Y2 (Rabbit polyclonal, 1:200, Almone labs: APR010AN0802, RRID: AB_2040078), GFAP (Chicken polyclonal, 1:2000, ThermoFisher Scientific: PA1‐10004, RRID: AB_1074620), PCNA (Mouse monoclonal, 1:1000, Abcam: ab29, RRID: AB_303394), or HuC/D (Mouse Monoclonal, 1:20 000, ThermoFisher Scientific: A‐21271, RRID: AB_221448).

Techniques: Analysis, Inhibition, Saline, Variant Assay, Injection

Immunohistochemistry showing PCNA and P2Y2 in transverse spinal cord cross sections. (A–C′) PCNA and P2Y2 distributions in naïve spinal cord tissue. (D–F′) PCNA and P2Y2 distributions 7 dpi on the rostral side of the injury. (G–I′) PCNA and P2Y2 distributions 7 dpi on the caudal side of the injury. Dorsal surface of the slices are oriented to the top of the image, the asterisk marks the central canal, and the white scale bar represents 50 μm. Arrows indicate co‐localization; dotted lines outline clearly definable membrane boundaries visible in the P2Y2 channel, with PCNA+ nuclei.

Journal: The FASEB Journal

Article Title: Purinergic Receptor P2Y2 Regulates Neurogenesis and Functional Recovery Following Spinal Cord Injury in Zebrafish

doi: 10.1096/fj.202504890RR

Figure Lengend Snippet: Immunohistochemistry showing PCNA and P2Y2 in transverse spinal cord cross sections. (A–C′) PCNA and P2Y2 distributions in naïve spinal cord tissue. (D–F′) PCNA and P2Y2 distributions 7 dpi on the rostral side of the injury. (G–I′) PCNA and P2Y2 distributions 7 dpi on the caudal side of the injury. Dorsal surface of the slices are oriented to the top of the image, the asterisk marks the central canal, and the white scale bar represents 50 μm. Arrows indicate co‐localization; dotted lines outline clearly definable membrane boundaries visible in the P2Y2 channel, with PCNA+ nuclei.

Article Snippet: All tissue sections were then incubated at room temperature overnight in a 0.1% Triton X‐100/0.01 M PBS solution containing at least one of the following primary antibodies: P2Y2 (Rabbit polyclonal, 1:200, Almone labs: APR010AN0802, RRID: AB_2040078), GFAP (Chicken polyclonal, 1:2000, ThermoFisher Scientific: PA1‐10004, RRID: AB_1074620), PCNA (Mouse monoclonal, 1:1000, Abcam: ab29, RRID: AB_303394), or HuC/D (Mouse Monoclonal, 1:20 000, ThermoFisher Scientific: A‐21271, RRID: AB_221448).

Techniques: Immunohistochemistry, Tissue, Membrane, Immunofluorescence

The impact of P2Y2 manipulation on proliferative cell counts at 7 dpi. (A) Schematic representation of treatment dosages and timeline. (B) For a naïve/non‐injured fish, 450 μm of thoracic spinal cord was analyzed. (C) For injured fish, 450 μm thoracic spinal cord immediately adjacent to the injury was analyzed from rostral and caudal sides. (D, E) Immunohistochemistry showing PCNA in transverse spinal cord cross sections in naïve fish. (F) Representative trace of PCNA + expression along the length of the spinal cord in the experimental conditions ( n = 1 per treatment). (G, H) Representative immunohistochemistry showing PCNA in transverse spinal cord cross sections from the rostral and caudal sides, respectively, of 7 dpi saline treated fish. (I, J) Quantitative analysis of mean PCNA + cell counts around the central canal within the first 450 μm rostral and caudal of the injury, normalized to naïve controls. AR‐C and saline vehicle groups significantly differed from naïve, both rostral (I; saline: p < 0.0001; AR‐C: p < 0.0001) and caudal (J; saline: p = 0.0005; AR‐C: p = 0.0002) of the injury. (K, L) Representative immunohistochemistry showing PCNA in transverse spinal cord cross sections taken from the rostral and caudal sides, respectively, of 7 dpi AR‐C treated fish. Dorsal surface of the slices are oriented to the top of the image, the asterisk marks the central canal, and the white scale bar represents 50 μm. Two‐way ANOVA (F) and one‐way ANOVA (IJ) used for analysis. Data presented as means ± SEM. ***Significant differences: p ≤ 0.001.

Journal: The FASEB Journal

Article Title: Purinergic Receptor P2Y2 Regulates Neurogenesis and Functional Recovery Following Spinal Cord Injury in Zebrafish

doi: 10.1096/fj.202504890RR

Figure Lengend Snippet: The impact of P2Y2 manipulation on proliferative cell counts at 7 dpi. (A) Schematic representation of treatment dosages and timeline. (B) For a naïve/non‐injured fish, 450 μm of thoracic spinal cord was analyzed. (C) For injured fish, 450 μm thoracic spinal cord immediately adjacent to the injury was analyzed from rostral and caudal sides. (D, E) Immunohistochemistry showing PCNA in transverse spinal cord cross sections in naïve fish. (F) Representative trace of PCNA + expression along the length of the spinal cord in the experimental conditions ( n = 1 per treatment). (G, H) Representative immunohistochemistry showing PCNA in transverse spinal cord cross sections from the rostral and caudal sides, respectively, of 7 dpi saline treated fish. (I, J) Quantitative analysis of mean PCNA + cell counts around the central canal within the first 450 μm rostral and caudal of the injury, normalized to naïve controls. AR‐C and saline vehicle groups significantly differed from naïve, both rostral (I; saline: p < 0.0001; AR‐C: p < 0.0001) and caudal (J; saline: p = 0.0005; AR‐C: p = 0.0002) of the injury. (K, L) Representative immunohistochemistry showing PCNA in transverse spinal cord cross sections taken from the rostral and caudal sides, respectively, of 7 dpi AR‐C treated fish. Dorsal surface of the slices are oriented to the top of the image, the asterisk marks the central canal, and the white scale bar represents 50 μm. Two‐way ANOVA (F) and one‐way ANOVA (IJ) used for analysis. Data presented as means ± SEM. ***Significant differences: p ≤ 0.001.

Article Snippet: All tissue sections were then incubated at room temperature overnight in a 0.1% Triton X‐100/0.01 M PBS solution containing at least one of the following primary antibodies: P2Y2 (Rabbit polyclonal, 1:200, Almone labs: APR010AN0802, RRID: AB_2040078), GFAP (Chicken polyclonal, 1:2000, ThermoFisher Scientific: PA1‐10004, RRID: AB_1074620), PCNA (Mouse monoclonal, 1:1000, Abcam: ab29, RRID: AB_303394), or HuC/D (Mouse Monoclonal, 1:20 000, ThermoFisher Scientific: A‐21271, RRID: AB_221448).

Techniques: Immunohistochemistry, Expressing, Saline, Analysis, Cell Characterization, Immunofluorescence

Immunohistochemistry showing HuC/D and P2Y2 in transverse spinal cord cross sections. (A–C′) HuC/D and P2Y2 distributions in naïve spinal cord tissue. (D–F′) HuC/D and P2Y2 distributions 14 dpi on the rostral side of the injury. (G–I′) HuC/D and P2Y2 distributions 14 dpi on the caudal side of the injury. Dorsal surface of the slice are oriented to the top of the image, the asterisk marks the central canal, and the white scale bar represents 50 μm. Arrows indicate examples of co‐localization.

Journal: The FASEB Journal

Article Title: Purinergic Receptor P2Y2 Regulates Neurogenesis and Functional Recovery Following Spinal Cord Injury in Zebrafish

doi: 10.1096/fj.202504890RR

Figure Lengend Snippet: Immunohistochemistry showing HuC/D and P2Y2 in transverse spinal cord cross sections. (A–C′) HuC/D and P2Y2 distributions in naïve spinal cord tissue. (D–F′) HuC/D and P2Y2 distributions 14 dpi on the rostral side of the injury. (G–I′) HuC/D and P2Y2 distributions 14 dpi on the caudal side of the injury. Dorsal surface of the slice are oriented to the top of the image, the asterisk marks the central canal, and the white scale bar represents 50 μm. Arrows indicate examples of co‐localization.

Article Snippet: All tissue sections were then incubated at room temperature overnight in a 0.1% Triton X‐100/0.01 M PBS solution containing at least one of the following primary antibodies: P2Y2 (Rabbit polyclonal, 1:200, Almone labs: APR010AN0802, RRID: AB_2040078), GFAP (Chicken polyclonal, 1:2000, ThermoFisher Scientific: PA1‐10004, RRID: AB_1074620), PCNA (Mouse monoclonal, 1:1000, Abcam: ab29, RRID: AB_303394), or HuC/D (Mouse Monoclonal, 1:20 000, ThermoFisher Scientific: A‐21271, RRID: AB_221448).

Techniques: Immunohistochemistry, Tissue, Immunofluorescence

The impact of P2Y2 manipulation on pan‐neuronal cell counts at 14 dpi. (A) Schematic representation of treatment dosages and timeline. (B) For a naïve/non‐injured fish, 450 μm of thoracic spinal cord was collected. (C) For injured fish, 450 μm thoracic spinal cord was collected immediately adjacent to the injury site on the rostral side, and 450 μm caudal. (D, E) Immunohistochemistry showing HuC/D in transverse spinal cord cross sections in naïve fish. (F) Representative trace of HuC/D + expression along the length of the spinal cord in the experimental conditions ( n = 1 per treatment). (G, H) Representative immunohistochemistry showing taken from the rostral and caudal sides of injury, respectively, of 14 dpi saline treated fish. (I) Quantitative analysis of mean rostral HuC/D + cell counts within the first 450 μm normalized to naïve controls, showing AR‐C significantly reduced HuC/D+ cell counts compared to the saline control group ( p = 0.0459). (J) Quantitative analysis of mean caudal HuC/D + cell counts around the central canal within the first 450 μm normalized to naïve controls, showing AR‐C significantly reduced HuC/D+ cell counts compared to the saline control group ( p = 0.0078). (K, L) Representative immunohistochemistry showing HuC/D in transverse spinal cord cross sections taken from the rostral and caudal sides of injury, respectively, of 14 dpi AR‐C treated fish. Dorsal surface of the slices are oriented to the top of the image, the asterisk marks the central canal, and the white scale bar represents 50 μm. Two‐way ANOVA (F) and one‐way ANOVA (IJ) used for analysis. Data presented as means ± SEM. *Significant differences: p ≤ 0.05. **Significant differences: p ≤ 0.01.

Journal: The FASEB Journal

Article Title: Purinergic Receptor P2Y2 Regulates Neurogenesis and Functional Recovery Following Spinal Cord Injury in Zebrafish

doi: 10.1096/fj.202504890RR

Figure Lengend Snippet: The impact of P2Y2 manipulation on pan‐neuronal cell counts at 14 dpi. (A) Schematic representation of treatment dosages and timeline. (B) For a naïve/non‐injured fish, 450 μm of thoracic spinal cord was collected. (C) For injured fish, 450 μm thoracic spinal cord was collected immediately adjacent to the injury site on the rostral side, and 450 μm caudal. (D, E) Immunohistochemistry showing HuC/D in transverse spinal cord cross sections in naïve fish. (F) Representative trace of HuC/D + expression along the length of the spinal cord in the experimental conditions ( n = 1 per treatment). (G, H) Representative immunohistochemistry showing taken from the rostral and caudal sides of injury, respectively, of 14 dpi saline treated fish. (I) Quantitative analysis of mean rostral HuC/D + cell counts within the first 450 μm normalized to naïve controls, showing AR‐C significantly reduced HuC/D+ cell counts compared to the saline control group ( p = 0.0459). (J) Quantitative analysis of mean caudal HuC/D + cell counts around the central canal within the first 450 μm normalized to naïve controls, showing AR‐C significantly reduced HuC/D+ cell counts compared to the saline control group ( p = 0.0078). (K, L) Representative immunohistochemistry showing HuC/D in transverse spinal cord cross sections taken from the rostral and caudal sides of injury, respectively, of 14 dpi AR‐C treated fish. Dorsal surface of the slices are oriented to the top of the image, the asterisk marks the central canal, and the white scale bar represents 50 μm. Two‐way ANOVA (F) and one‐way ANOVA (IJ) used for analysis. Data presented as means ± SEM. *Significant differences: p ≤ 0.05. **Significant differences: p ≤ 0.01.

Article Snippet: All tissue sections were then incubated at room temperature overnight in a 0.1% Triton X‐100/0.01 M PBS solution containing at least one of the following primary antibodies: P2Y2 (Rabbit polyclonal, 1:200, Almone labs: APR010AN0802, RRID: AB_2040078), GFAP (Chicken polyclonal, 1:2000, ThermoFisher Scientific: PA1‐10004, RRID: AB_1074620), PCNA (Mouse monoclonal, 1:1000, Abcam: ab29, RRID: AB_303394), or HuC/D (Mouse Monoclonal, 1:20 000, ThermoFisher Scientific: A‐21271, RRID: AB_221448).

Techniques: Immunohistochemistry, Expressing, Saline, Analysis, Control, Cell Characterization, Immunofluorescence