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(A) Mean live cell counts of wild‐type and deletion mutant cultures in log10(CFU/mL) ± SD over 168‐h corrosion assays. Wild type: n = 4, ∆ phz : n = 4, ∆ hcn : n = 4, ∆ phz ∆ hcn : n = 4. (B) Dose‐response curve reporting growth rate of P. chlororaphis subspecies aureofaciens in response to treatment with Co(2+) concentrations ranging from 0 to 2000 μM (presented on a log10 scale). Dose‐response curves were fitted by calculating growth rates in Excel using the slopes of the linear portions of each growth curve. These values were fitted with a 4‐parameter log‐logistic equation using the ‘drc’ package in R. The calculated IC50 value was 14.79 ± 0.86 μM. (C) Distribution of free, cell‐adsorbed and intracellular Co(2+) in 10% (v/v) inoculated wild‐type cultures spiked with Co(2+) at concentrations of 20, 200 and 2000 μM, respectively. These concentrations were calculated from Co(2+) recovered by A‐F, F‐A and EDTA rinse treatments (see Section ). Co(2+) concentrations for 0 and 2 μM were not included since none of the Co(2+) recovered was cell‐associated.

Journal: Environmental Microbiology

Article Title: Microbially‐Induced Corrosion of Cobalt by Pseudomonas chlororaphis subspecies aureofaciens Is Driven by the Complex Interplay Between Abiotic and Biotic Processes That Control Cobalt Solubility

doi: 10.1111/1462-2920.70441

Figure Lengend Snippet: (A) Mean live cell counts of wild‐type and deletion mutant cultures in log10(CFU/mL) ± SD over 168‐h corrosion assays. Wild type: n = 4, ∆ phz : n = 4, ∆ hcn : n = 4, ∆ phz ∆ hcn : n = 4. (B) Dose‐response curve reporting growth rate of P. chlororaphis subspecies aureofaciens in response to treatment with Co(2+) concentrations ranging from 0 to 2000 μM (presented on a log10 scale). Dose‐response curves were fitted by calculating growth rates in Excel using the slopes of the linear portions of each growth curve. These values were fitted with a 4‐parameter log‐logistic equation using the ‘drc’ package in R. The calculated IC50 value was 14.79 ± 0.86 μM. (C) Distribution of free, cell‐adsorbed and intracellular Co(2+) in 10% (v/v) inoculated wild‐type cultures spiked with Co(2+) at concentrations of 20, 200 and 2000 μM, respectively. These concentrations were calculated from Co(2+) recovered by A‐F, F‐A and EDTA rinse treatments (see Section ). Co(2+) concentrations for 0 and 2 μM were not included since none of the Co(2+) recovered was cell‐associated.

Article Snippet: P. chlororaphis subsp. aureofaciens (ATCC 13985) , Wild‐type P. chlororaphis subspecies aureofaciens strain , (Kluyver ), ATCC .

Techniques: Mutagenesis

Homology heatmap for genes involved in microbially‐induced corrosion (MIC) or the redox cycling of solid iron species via direct electron transfer (DET). Gene homologues were annotated using a custom database input into DRAM, which carries out a BLAST‐style homology search where hits that passed a bit‐score of 60 are kept. Blank cells indicate that no hits of sufficient quality were obtained for those genes. Genomes from the model organisms in which the genes were identified are included as positive controls for comparison to the Pseudomonas chlororaphis subspecies aureofaciens wild‐type (WT) genome.

Journal: Environmental Microbiology

Article Title: Microbially‐Induced Corrosion of Cobalt by Pseudomonas chlororaphis subspecies aureofaciens Is Driven by the Complex Interplay Between Abiotic and Biotic Processes That Control Cobalt Solubility

doi: 10.1111/1462-2920.70441

Figure Lengend Snippet: Homology heatmap for genes involved in microbially‐induced corrosion (MIC) or the redox cycling of solid iron species via direct electron transfer (DET). Gene homologues were annotated using a custom database input into DRAM, which carries out a BLAST‐style homology search where hits that passed a bit‐score of 60 are kept. Blank cells indicate that no hits of sufficient quality were obtained for those genes. Genomes from the model organisms in which the genes were identified are included as positive controls for comparison to the Pseudomonas chlororaphis subspecies aureofaciens wild‐type (WT) genome.

Article Snippet: P. chlororaphis subsp. aureofaciens (ATCC 13985) , Wild‐type P. chlororaphis subspecies aureofaciens strain , (Kluyver ), ATCC .

Techniques: Model Organisms, Comparison

Summary of strains, plasmids and primers used in genetic manipulations.

Journal: Environmental Microbiology

Article Title: Microbially‐Induced Corrosion of Cobalt by Pseudomonas chlororaphis subspecies aureofaciens Is Driven by the Complex Interplay Between Abiotic and Biotic Processes That Control Cobalt Solubility

doi: 10.1111/1462-2920.70441

Figure Lengend Snippet: Summary of strains, plasmids and primers used in genetic manipulations.

Article Snippet: P. chlororaphis subsp. aureofaciens (ATCC 13985) , Wild‐type P. chlororaphis subspecies aureofaciens strain , (Kluyver ), ATCC .

Techniques: Mutagenesis