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Multi-enzyme activity and ROS-responsive platform construction. (A) OXD-like enzyme activity at pH = 5.5. (B) OXD-like enzyme activity of MMBOx under different pH conditions. (C) POD-like enzyme activity at pH = 5.5. (D) POD-like enzyme activity of MMBOx under different pH conditions. (E) CAT-like enzyme activity at pH = 7.4. (F) CAT-like enzyme activity in five cycles for MMBOx. (G) K m and V max of MMBOx. (H) CAT-like enzyme activity of MMBOx under different pH conditions. (I) SOD-like enzyme activity under different concentration conditions at pH = 7.4. (J) SOD-like enzyme activity in five cycles for MMBOx. (K) Michaelis-Menten curves measured by EST-8 method for MMBOx. (L) DPPH scavenging curves at pH = 7.4. (M) Schematic diagrams of the mechanism of activation of enzyme activities by MMBOx in different environments. (N) Schematic illustration of GelMA-PBA synthesis and dual-network hydrogel formation. (O) Photographs of 3D-printed scaffolds with BOx and MMBOx incorporation (scale bars: 2 mm). (P) Storage modulus (G′) and loss modulus (G″) of different groups. (Q) Stress-strain curves of hydrogels. (R) Swelling kinetics of hydrogels. (S) <t>H</t> <t>2</t> <t>O</t> 2 scavenging efficiency of different groups.
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Effect of myrtenol, curcumin, and oridonin on RNP granule pH, cellular translation, and senescence (A) Schematic of treatment with natural products for H 2 O 2 -senescent cells. (B) pH levels of P-bodies and SGs within H 2 O 2 -senescent cells pre-treated with natural products. Data are represented as mean ± SD ( n = 5 cells with at least 20 granules). (C–F) Effect of natural products on SA-β-Gal activity (C), cell viability (D), and protein synthesis activity (E and F) in H 2 O 2 -senescent cells. Data in (C)–(E) are represented as mean ± SD ( n = 3). Images were representative examples from three independent experiments. Scale bars in (F), 40 μm. Statistical significance was assessed using a non-paired two-tailed t test in (B)–(E) (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001).
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Multi-enzyme activity and ROS-responsive platform construction. (A) OXD-like enzyme activity at pH = 5.5. (B) OXD-like enzyme activity of MMBOx under different pH conditions. (C) POD-like enzyme activity at pH = 5.5. (D) POD-like enzyme activity of MMBOx under different pH conditions. (E) CAT-like enzyme activity at pH = 7.4. (F) CAT-like enzyme activity in five cycles for MMBOx. (G) K m and V max of MMBOx. (H) CAT-like enzyme activity of MMBOx under different pH conditions. (I) SOD-like enzyme activity under different concentration conditions at pH = 7.4. (J) SOD-like enzyme activity in five cycles for MMBOx. (K) Michaelis-Menten curves measured by EST-8 method for MMBOx. (L) DPPH scavenging curves at pH = 7.4. (M) Schematic diagrams of the mechanism of activation of enzyme activities by MMBOx in different environments. (N) Schematic illustration of GelMA-PBA synthesis and dual-network hydrogel formation. (O) Photographs of 3D-printed scaffolds with BOx and MMBOx incorporation (scale bars: 2 mm). (P) Storage modulus (G′) and loss modulus (G″) of different groups. (Q) Stress-strain curves of hydrogels. (R) Swelling kinetics of hydrogels. (S) H 2 O 2 scavenging efficiency of different groups.

Journal: Bioactive Materials

Article Title: A multimodal ROS logic-gated therapeutic platform disrupts the vicious cycle of senescence to promote aged bone defect repair

doi: 10.1016/j.bioactmat.2026.02.002

Figure Lengend Snippet: Multi-enzyme activity and ROS-responsive platform construction. (A) OXD-like enzyme activity at pH = 5.5. (B) OXD-like enzyme activity of MMBOx under different pH conditions. (C) POD-like enzyme activity at pH = 5.5. (D) POD-like enzyme activity of MMBOx under different pH conditions. (E) CAT-like enzyme activity at pH = 7.4. (F) CAT-like enzyme activity in five cycles for MMBOx. (G) K m and V max of MMBOx. (H) CAT-like enzyme activity of MMBOx under different pH conditions. (I) SOD-like enzyme activity under different concentration conditions at pH = 7.4. (J) SOD-like enzyme activity in five cycles for MMBOx. (K) Michaelis-Menten curves measured by EST-8 method for MMBOx. (L) DPPH scavenging curves at pH = 7.4. (M) Schematic diagrams of the mechanism of activation of enzyme activities by MMBOx in different environments. (N) Schematic illustration of GelMA-PBA synthesis and dual-network hydrogel formation. (O) Photographs of 3D-printed scaffolds with BOx and MMBOx incorporation (scale bars: 2 mm). (P) Storage modulus (G′) and loss modulus (G″) of different groups. (Q) Stress-strain curves of hydrogels. (R) Swelling kinetics of hydrogels. (S) H 2 O 2 scavenging efficiency of different groups.

Article Snippet: NaBiO 3 , NaOH, MgCl 2 , MnCl 2 ·H 2 O, MB, DPBF were from Macklin Biochemical Technology Co., Ltd (Shanghai, China).

Techniques: Activity Assay, Concentration Assay, Activation Assay

Effect of myrtenol, curcumin, and oridonin on RNP granule pH, cellular translation, and senescence (A) Schematic of treatment with natural products for H 2 O 2 -senescent cells. (B) pH levels of P-bodies and SGs within H 2 O 2 -senescent cells pre-treated with natural products. Data are represented as mean ± SD ( n = 5 cells with at least 20 granules). (C–F) Effect of natural products on SA-β-Gal activity (C), cell viability (D), and protein synthesis activity (E and F) in H 2 O 2 -senescent cells. Data in (C)–(E) are represented as mean ± SD ( n = 3). Images were representative examples from three independent experiments. Scale bars in (F), 40 μm. Statistical significance was assessed using a non-paired two-tailed t test in (B)–(E) (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001).

Journal: iScience

Article Title: Single-granule profiling reveals that RNP granule pH marks cellular translation

doi: 10.1016/j.isci.2026.116203

Figure Lengend Snippet: Effect of myrtenol, curcumin, and oridonin on RNP granule pH, cellular translation, and senescence (A) Schematic of treatment with natural products for H 2 O 2 -senescent cells. (B) pH levels of P-bodies and SGs within H 2 O 2 -senescent cells pre-treated with natural products. Data are represented as mean ± SD ( n = 5 cells with at least 20 granules). (C–F) Effect of natural products on SA-β-Gal activity (C), cell viability (D), and protein synthesis activity (E and F) in H 2 O 2 -senescent cells. Data in (C)–(E) are represented as mean ± SD ( n = 3). Images were representative examples from three independent experiments. Scale bars in (F), 40 μm. Statistical significance was assessed using a non-paired two-tailed t test in (B)–(E) (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001).

Article Snippet: H 2 O 2 , Macklin , Cat# H792077.

Techniques: Activity Assay, Two Tailed Test

Journal: iScience

Article Title: Single-granule profiling reveals that RNP granule pH marks cellular translation

doi: 10.1016/j.isci.2026.116203

Figure Lengend Snippet:

Article Snippet: H 2 O 2 , Macklin , Cat# H792077.

Techniques: Recombinant, Software