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human nectin1 protein  (MedChemExpress)
94
MedChemExpress human nectin1 protein
Nectin4 competitively attenuates gD and <t>Nectin1</t> interaction. ( A ) HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-empty vector or Myc-PRV-gD, and either full-length or mutant Nectin4 plasmid. Protein samples were lysed at 4°C and incubated with Myc-magnetic beads for 2 h. The Nectin1-PRV-gD interaction was assessed by Western blot. The interaction between Nectin1 and gD was evaluated under different doses of Nectin4 and its mutants. HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-tagged PRV-gD, and increasing amounts of full-length Nectin4 ( B ), V5-tagged IgV-deletion ( C ), or V5-tagged IgC2 deletion mutants ( D ). The RFP control vector was included as a negative control. Cell lysates were incubated with Myc-magnetic beads at 4°C for 2 h, and the Nectin1-gD interaction was analyzed by Western blot. ( E ) The Nectin1-gD interaction under increasing doses of Nectin4 was determined by SPR. The Nectin4 protein was injected at increasing concentrations onto a surface precoated with a saturated mixture of Nectin1 and PRV-gD proteins. The binding affinity between PRV-gD and Nectin1 was measured by monitoring changes in resonance units (RU) in response to Nectin4 binding.
Human Nectin1 Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human nectin1 protein - by Bioz Stars, 2026-08
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human nectin-4 antibody  (Bio-Techne corporation)
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Bio-Techne corporation human nectin-4 antibody
Nectin4 competitively attenuates gD and <t>Nectin1</t> interaction. ( A ) HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-empty vector or Myc-PRV-gD, and either full-length or mutant Nectin4 plasmid. Protein samples were lysed at 4°C and incubated with Myc-magnetic beads for 2 h. The Nectin1-PRV-gD interaction was assessed by Western blot. The interaction between Nectin1 and gD was evaluated under different doses of Nectin4 and its mutants. HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-tagged PRV-gD, and increasing amounts of full-length Nectin4 ( B ), V5-tagged IgV-deletion ( C ), or V5-tagged IgC2 deletion mutants ( D ). The RFP control vector was included as a negative control. Cell lysates were incubated with Myc-magnetic beads at 4°C for 2 h, and the Nectin1-gD interaction was analyzed by Western blot. ( E ) The Nectin1-gD interaction under increasing doses of Nectin4 was determined by SPR. The Nectin4 protein was injected at increasing concentrations onto a surface precoated with a saturated mixture of Nectin1 and PRV-gD proteins. The binding affinity between PRV-gD and Nectin1 was measured by monitoring changes in resonance units (RU) in response to Nectin4 binding.
Human Nectin 4 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd111 nectin  (Sino Biological)
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Sino Biological cd111 nectin
Nectin4 competitively attenuates gD and <t>Nectin1</t> interaction. ( A ) HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-empty vector or Myc-PRV-gD, and either full-length or mutant Nectin4 plasmid. Protein samples were lysed at 4°C and incubated with Myc-magnetic beads for 2 h. The Nectin1-PRV-gD interaction was assessed by Western blot. The interaction between Nectin1 and gD was evaluated under different doses of Nectin4 and its mutants. HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-tagged PRV-gD, and increasing amounts of full-length Nectin4 ( B ), V5-tagged IgV-deletion ( C ), or V5-tagged IgC2 deletion mutants ( D ). The RFP control vector was included as a negative control. Cell lysates were incubated with Myc-magnetic beads at 4°C for 2 h, and the Nectin1-gD interaction was analyzed by Western blot. ( E ) The Nectin1-gD interaction under increasing doses of Nectin4 was determined by SPR. The Nectin4 protein was injected at increasing concentrations onto a surface precoated with a saturated mixture of Nectin1 and PRV-gD proteins. The binding affinity between PRV-gD and Nectin1 was measured by monitoring changes in resonance units (RU) in response to Nectin4 binding.
Cd111 Nectin, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd111 nectin - by Bioz Stars, 2026-08
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nectins  (Okabe Co Ltd)
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Okabe Co Ltd nectins
Nectin4 competitively attenuates gD and <t>Nectin1</t> interaction. ( A ) HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-empty vector or Myc-PRV-gD, and either full-length or mutant Nectin4 plasmid. Protein samples were lysed at 4°C and incubated with Myc-magnetic beads for 2 h. The Nectin1-PRV-gD interaction was assessed by Western blot. The interaction between Nectin1 and gD was evaluated under different doses of Nectin4 and its mutants. HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-tagged PRV-gD, and increasing amounts of full-length Nectin4 ( B ), V5-tagged IgV-deletion ( C ), or V5-tagged IgC2 deletion mutants ( D ). The RFP control vector was included as a negative control. Cell lysates were incubated with Myc-magnetic beads at 4°C for 2 h, and the Nectin1-gD interaction was analyzed by Western blot. ( E ) The Nectin1-gD interaction under increasing doses of Nectin4 was determined by SPR. The Nectin4 protein was injected at increasing concentrations onto a surface precoated with a saturated mixture of Nectin1 and PRV-gD proteins. The binding affinity between PRV-gD and Nectin1 was measured by monitoring changes in resonance units (RU) in response to Nectin4 binding.
Nectins, supplied by Okabe Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti nectin 4 antibody drug conjugate against nectin 4 positive triple negative breast cancer models fabrice ngoh njotu  (Oncologie Inc)
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Oncologie Inc anti nectin 4 antibody drug conjugate against nectin 4 positive triple negative breast cancer models fabrice ngoh njotu
Nectin4 competitively attenuates gD and <t>Nectin1</t> interaction. ( A ) HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-empty vector or Myc-PRV-gD, and either full-length or mutant Nectin4 plasmid. Protein samples were lysed at 4°C and incubated with Myc-magnetic beads for 2 h. The Nectin1-PRV-gD interaction was assessed by Western blot. The interaction between Nectin1 and gD was evaluated under different doses of Nectin4 and its mutants. HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-tagged PRV-gD, and increasing amounts of full-length Nectin4 ( B ), V5-tagged IgV-deletion ( C ), or V5-tagged IgC2 deletion mutants ( D ). The RFP control vector was included as a negative control. Cell lysates were incubated with Myc-magnetic beads at 4°C for 2 h, and the Nectin1-gD interaction was analyzed by Western blot. ( E ) The Nectin1-gD interaction under increasing doses of Nectin4 was determined by SPR. The Nectin4 protein was injected at increasing concentrations onto a surface precoated with a saturated mixture of Nectin1 and PRV-gD proteins. The binding affinity between PRV-gD and Nectin1 was measured by monitoring changes in resonance units (RU) in response to Nectin4 binding.
Anti Nectin 4 Antibody Drug Conjugate Against Nectin 4 Positive Triple Negative Breast Cancer Models Fabrice Ngoh Njotu, supplied by Oncologie Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti nectin 4 antibody drug conjugate against nectin 4 positive triple negative breast cancer models fabrice ngoh njotu - by Bioz Stars, 2026-08
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nectin 4 directed mab conjugated  (Astellas)
86
Astellas nectin 4 directed mab conjugated
Nectin4 competitively attenuates gD and <t>Nectin1</t> interaction. ( A ) HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-empty vector or Myc-PRV-gD, and either full-length or mutant Nectin4 plasmid. Protein samples were lysed at 4°C and incubated with Myc-magnetic beads for 2 h. The Nectin1-PRV-gD interaction was assessed by Western blot. The interaction between Nectin1 and gD was evaluated under different doses of Nectin4 and its mutants. HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-tagged PRV-gD, and increasing amounts of full-length Nectin4 ( B ), V5-tagged IgV-deletion ( C ), or V5-tagged IgC2 deletion mutants ( D ). The RFP control vector was included as a negative control. Cell lysates were incubated with Myc-magnetic beads at 4°C for 2 h, and the Nectin1-gD interaction was analyzed by Western blot. ( E ) The Nectin1-gD interaction under increasing doses of Nectin4 was determined by SPR. The Nectin4 protein was injected at increasing concentrations onto a surface precoated with a saturated mixture of Nectin1 and PRV-gD proteins. The binding affinity between PRV-gD and Nectin1 was measured by monitoring changes in resonance units (RU) in response to Nectin4 binding.
Nectin 4 Directed Mab Conjugated, supplied by Astellas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nectin 4 directed mab conjugated - by Bioz Stars, 2026-08
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anti nectin 4 antibodies  (Inserm Transfert)
86
Inserm Transfert anti nectin 4 antibodies
Nectin4 competitively attenuates gD and <t>Nectin1</t> interaction. ( A ) HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-empty vector or Myc-PRV-gD, and either full-length or mutant Nectin4 plasmid. Protein samples were lysed at 4°C and incubated with Myc-magnetic beads for 2 h. The Nectin1-PRV-gD interaction was assessed by Western blot. The interaction between Nectin1 and gD was evaluated under different doses of Nectin4 and its mutants. HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-tagged PRV-gD, and increasing amounts of full-length Nectin4 ( B ), V5-tagged IgV-deletion ( C ), or V5-tagged IgC2 deletion mutants ( D ). The RFP control vector was included as a negative control. Cell lysates were incubated with Myc-magnetic beads at 4°C for 2 h, and the Nectin1-gD interaction was analyzed by Western blot. ( E ) The Nectin1-gD interaction under increasing doses of Nectin4 was determined by SPR. The Nectin4 protein was injected at increasing concentrations onto a surface precoated with a saturated mixture of Nectin1 and PRV-gD proteins. The binding affinity between PRV-gD and Nectin1 was measured by monitoring changes in resonance units (RU) in response to Nectin4 binding.
Anti Nectin 4 Antibodies, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti nectin 4 targeting agent  (Bicycle Therapeutics)
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Bicycle Therapeutics anti nectin 4 targeting agent
Nectin4 competitively attenuates gD and <t>Nectin1</t> interaction. ( A ) HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-empty vector or Myc-PRV-gD, and either full-length or mutant Nectin4 plasmid. Protein samples were lysed at 4°C and incubated with Myc-magnetic beads for 2 h. The Nectin1-PRV-gD interaction was assessed by Western blot. The interaction between Nectin1 and gD was evaluated under different doses of Nectin4 and its mutants. HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-tagged PRV-gD, and increasing amounts of full-length Nectin4 ( B ), V5-tagged IgV-deletion ( C ), or V5-tagged IgC2 deletion mutants ( D ). The RFP control vector was included as a negative control. Cell lysates were incubated with Myc-magnetic beads at 4°C for 2 h, and the Nectin1-gD interaction was analyzed by Western blot. ( E ) The Nectin1-gD interaction under increasing doses of Nectin4 was determined by SPR. The Nectin4 protein was injected at increasing concentrations onto a surface precoated with a saturated mixture of Nectin1 and PRV-gD proteins. The binding affinity between PRV-gD and Nectin1 was measured by monitoring changes in resonance units (RU) in response to Nectin4 binding.
Anti Nectin 4 Targeting Agent, supplied by Bicycle Therapeutics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human nectin 4  (Novoprotein)
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Novoprotein human nectin 4
Nectin4 competitively attenuates gD and <t>Nectin1</t> interaction. ( A ) HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-empty vector or Myc-PRV-gD, and either full-length or mutant Nectin4 plasmid. Protein samples were lysed at 4°C and incubated with Myc-magnetic beads for 2 h. The Nectin1-PRV-gD interaction was assessed by Western blot. The interaction between Nectin1 and gD was evaluated under different doses of Nectin4 and its mutants. HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-tagged PRV-gD, and increasing amounts of full-length Nectin4 ( B ), V5-tagged IgV-deletion ( C ), or V5-tagged IgC2 deletion mutants ( D ). The RFP control vector was included as a negative control. Cell lysates were incubated with Myc-magnetic beads at 4°C for 2 h, and the Nectin1-gD interaction was analyzed by Western blot. ( E ) The Nectin1-gD interaction under increasing doses of Nectin4 was determined by SPR. The Nectin4 protein was injected at increasing concentrations onto a surface precoated with a saturated mixture of Nectin1 and PRV-gD proteins. The binding affinity between PRV-gD and Nectin1 was measured by monitoring changes in resonance units (RU) in response to Nectin4 binding.
Human Nectin 4, supplied by Novoprotein, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human nectin 2  (Novoprotein)
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Novoprotein human nectin 2
Nectin4 competitively attenuates gD and <t>Nectin1</t> interaction. ( A ) HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-empty vector or Myc-PRV-gD, and either full-length or mutant Nectin4 plasmid. Protein samples were lysed at 4°C and incubated with Myc-magnetic beads for 2 h. The Nectin1-PRV-gD interaction was assessed by Western blot. The interaction between Nectin1 and gD was evaluated under different doses of Nectin4 and its mutants. HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-tagged PRV-gD, and increasing amounts of full-length Nectin4 ( B ), V5-tagged IgV-deletion ( C ), or V5-tagged IgC2 deletion mutants ( D ). The RFP control vector was included as a negative control. Cell lysates were incubated with Myc-magnetic beads at 4°C for 2 h, and the Nectin1-gD interaction was analyzed by Western blot. ( E ) The Nectin1-gD interaction under increasing doses of Nectin4 was determined by SPR. The Nectin4 protein was injected at increasing concentrations onto a surface precoated with a saturated mixture of Nectin1 and PRV-gD proteins. The binding affinity between PRV-gD and Nectin1 was measured by monitoring changes in resonance units (RU) in response to Nectin4 binding.
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Image Search Results


Nectin4 competitively attenuates gD and Nectin1 interaction. ( A ) HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-empty vector or Myc-PRV-gD, and either full-length or mutant Nectin4 plasmid. Protein samples were lysed at 4°C and incubated with Myc-magnetic beads for 2 h. The Nectin1-PRV-gD interaction was assessed by Western blot. The interaction between Nectin1 and gD was evaluated under different doses of Nectin4 and its mutants. HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-tagged PRV-gD, and increasing amounts of full-length Nectin4 ( B ), V5-tagged IgV-deletion ( C ), or V5-tagged IgC2 deletion mutants ( D ). The RFP control vector was included as a negative control. Cell lysates were incubated with Myc-magnetic beads at 4°C for 2 h, and the Nectin1-gD interaction was analyzed by Western blot. ( E ) The Nectin1-gD interaction under increasing doses of Nectin4 was determined by SPR. The Nectin4 protein was injected at increasing concentrations onto a surface precoated with a saturated mixture of Nectin1 and PRV-gD proteins. The binding affinity between PRV-gD and Nectin1 was measured by monitoring changes in resonance units (RU) in response to Nectin4 binding.

Journal: Journal of Virology

Article Title: Nectin4 restricts pseudorabies virus infection by blocking gD-Nectin1 interaction

doi: 10.1128/jvi.00788-26

Figure Lengend Snippet: Nectin4 competitively attenuates gD and Nectin1 interaction. ( A ) HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-empty vector or Myc-PRV-gD, and either full-length or mutant Nectin4 plasmid. Protein samples were lysed at 4°C and incubated with Myc-magnetic beads for 2 h. The Nectin1-PRV-gD interaction was assessed by Western blot. The interaction between Nectin1 and gD was evaluated under different doses of Nectin4 and its mutants. HEK293T cells were cotransfected with Flag-tagged Nectin1, Myc-tagged PRV-gD, and increasing amounts of full-length Nectin4 ( B ), V5-tagged IgV-deletion ( C ), or V5-tagged IgC2 deletion mutants ( D ). The RFP control vector was included as a negative control. Cell lysates were incubated with Myc-magnetic beads at 4°C for 2 h, and the Nectin1-gD interaction was analyzed by Western blot. ( E ) The Nectin1-gD interaction under increasing doses of Nectin4 was determined by SPR. The Nectin4 protein was injected at increasing concentrations onto a surface precoated with a saturated mixture of Nectin1 and PRV-gD proteins. The binding affinity between PRV-gD and Nectin1 was measured by monitoring changes in resonance units (RU) in response to Nectin4 binding.

Article Snippet: The purified human Nectin1 protein was purchased from MedChemExpress (HY- P70494 ).

Techniques: Plasmid Preparation, Mutagenesis, Incubation, Magnetic Beads, Western Blot, Control, Negative Control, Injection, Binding Assay

Schematic illustrating the mechanism by which Nectin4 restricts pseudorabies virus infection through blocking gD-Nectin1 interaction. PRV infection involves multiple steps, including attachment, internalization, membrane fusion, and genome release. Initial attachment is primarily mediated through interactions of viral glycoprotein gC with cell-surface heparan sulfate proteoglycans. Receptor binding by gD initiates the fusion cascade and viral internalization. Upon PRV infection, the interferon signaling pathway is activated (left), subsequently upregulating the expression of interferon-stimulated gene Nectin4 in neighboring cells. Nectin4 is located on the cell membrane and binds to gD through its IgV domain, preventing viral internalization (right). Figure created with BioRender (biorender.com).

Journal: Journal of Virology

Article Title: Nectin4 restricts pseudorabies virus infection by blocking gD-Nectin1 interaction

doi: 10.1128/jvi.00788-26

Figure Lengend Snippet: Schematic illustrating the mechanism by which Nectin4 restricts pseudorabies virus infection through blocking gD-Nectin1 interaction. PRV infection involves multiple steps, including attachment, internalization, membrane fusion, and genome release. Initial attachment is primarily mediated through interactions of viral glycoprotein gC with cell-surface heparan sulfate proteoglycans. Receptor binding by gD initiates the fusion cascade and viral internalization. Upon PRV infection, the interferon signaling pathway is activated (left), subsequently upregulating the expression of interferon-stimulated gene Nectin4 in neighboring cells. Nectin4 is located on the cell membrane and binds to gD through its IgV domain, preventing viral internalization (right). Figure created with BioRender (biorender.com).

Article Snippet: The purified human Nectin1 protein was purchased from MedChemExpress (HY- P70494 ).

Techniques: Virus, Infection, Blocking Assay, Membrane, Binding Assay, Expressing