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ubiquitin p4d1  (Novus Biologicals)


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    Structured Review

    Novus Biologicals ubiquitin p4d1
    Ubiquitin P4d1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nb300/Ubiquitin+Antibody+(Ubi-1)/pmc13122179-106-42-47
    Average 94 stars, based on 47 article reviews
    ubiquitin p4d1 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Endothelial FUNDC1 regulates metabolic reprogramming and the obesity-diabetes transition through the SIRT3/GATA2/endothelin-1 axis
    Article Snippet: .. The aortic sections were incubated with FUNDC1 (Biorbyt, orb156964), 8-OHdG (abcam, ab62623), or ET-1 (Novus, NB300-526) antibody at 4 °C overnight and followed by incubation with EnVision® + Dual Link System-HRP (Dako Cytomation, 3468) and the signal was visualized using the Liquid DAB+ Substrate Chromogen System and imaged using an inverted phase contrast microscope (Nikon, Tokyo, Japan). .. The staining density and area in EC layer (the area between lumen and internal elastic layer) and adipose tissue were measured using ImageJ software (NIH).

    Article Title: Endothelial FUNDC1 regulates metabolic reprogramming and the obesity-diabetes transition through the SIRT3/GATA2/endothelin-1 axis.
    Article Snippet: .. The aortic sections were incubated with FUNDC1 (Biorbyt, orb156964), 8-OHdG (abcam, ab62623), or ET-1 (Novus, NB300-526) antibody at 4C overnight and followed by incubation with EnVision® + Dual Link System-HRP (Dako Cytomation, 3468) and the signal was visualized using the Liquid DAB+ Substrate Chromogen System and imaged using an inverted phase contrast microscope (Nikon, Tokyo, Japan). .. The staining density and area in EC layer (the area between lumen and internal elastic layer) and adipose tissue were measured using ImageJ software (NIH).

    Microscopy:

    Article Title: Endothelial FUNDC1 regulates metabolic reprogramming and the obesity-diabetes transition through the SIRT3/GATA2/endothelin-1 axis
    Article Snippet: .. The aortic sections were incubated with FUNDC1 (Biorbyt, orb156964), 8-OHdG (abcam, ab62623), or ET-1 (Novus, NB300-526) antibody at 4 °C overnight and followed by incubation with EnVision® + Dual Link System-HRP (Dako Cytomation, 3468) and the signal was visualized using the Liquid DAB+ Substrate Chromogen System and imaged using an inverted phase contrast microscope (Nikon, Tokyo, Japan). .. The staining density and area in EC layer (the area between lumen and internal elastic layer) and adipose tissue were measured using ImageJ software (NIH).

    Article Title: Endothelial FUNDC1 regulates metabolic reprogramming and the obesity-diabetes transition through the SIRT3/GATA2/endothelin-1 axis.
    Article Snippet: .. The aortic sections were incubated with FUNDC1 (Biorbyt, orb156964), 8-OHdG (abcam, ab62623), or ET-1 (Novus, NB300-526) antibody at 4C overnight and followed by incubation with EnVision® + Dual Link System-HRP (Dako Cytomation, 3468) and the signal was visualized using the Liquid DAB+ Substrate Chromogen System and imaged using an inverted phase contrast microscope (Nikon, Tokyo, Japan). .. The staining density and area in EC layer (the area between lumen and internal elastic layer) and adipose tissue were measured using ImageJ software (NIH).

    Generated:

    Article Title: Tachykinin signaling defines distinct populations of glia in the enteric nervous system.
    Article Snippet: Article Tachykinin signaling defines distinct populations of glia in the enteric nervous system

    Recombinant:

    Article Title: Tachykinin signaling defines distinct populations of glia in the enteric nervous system.
    Article Snippet: Article Tachykinin signaling defines distinct populations of glia in the enteric nervous system

    Article Title: Modeling spastic paraplegia 4 with corticospinal motor neuron-enriched cortical organoids reveals genotype-phenotype and HDAC6-targetable pathology.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies SOX2 R&D Systems Cat# AF2018; RRID:AB_355110 Neun Abcam Cat# ab104224; RRID:AB_10711040 MAP2 Novus Biologicals NB300-213 Cat# NB300-213; RRID:AB_2138178 βIII- Tubulin BioLegend Cat# 802001; RRID:AB_2564645 CTIP2 Abcam Cat# ab18465; RRID:AB_2064130 GFAP Abcam Cat# ab53554; RRID:AB_880202 SMI312 BioLegend Cat# 837904; RRID:AB_2566782 L1CAM BioLegend Cat# 371607; RRID:AB_2734424 vGlut1 Synaptic Systems Cat# 135 302; RRID:AB_887877 Synaptophysin Proteintech Proteintech Cat# 60191-1-Ig; RRID:AB_10915965 SMI32 BioLegend Cat# 801701; RRID:AB_2564642 Acetylated Tubulin Sigma-Aldrich Cat# T6793; RRID:AB_477585 HDAC6 Cell Signaling Technology Cat# 7612; RRID:AB_10889735 βIII- Tubulin Abcam Cat# ab7751; RRID:AB_306045 GAPDH Abcam Cat# ab8245; RRID:AB_2107448 Spastin Abcam Cat# ab77144; RRID:AB_1524436 Anti-Flag Proteintech Cat# 20543-1-AP; RRID:AB_11232216 Anti-Phosphoserine/threonine PhosphoSolutions Cat# PP2551; RRID:AB_1184778 Anti-HDAC6 Affinity Biosciences Cat# AF6485; RRID:AB_2835165 Anti-mCherry Abcam Cat# ab205402; RRID:AB_2722769 Chemicals, peptides, and recombinant proteins MTeSR TM Plus Stem Cell Technologies 100–0276 DMEM-F12 Gibco 11330032 ReLeSR TM Stem Cell Technologies 100–0483 ROCK inhibitor Y-27632 Tocris 1254 Dorsomorphin Sigma C956T21 SB431542 Tocris 1614 Neurobasal A Thermo Fisher 10-888- 022 B-27 without Vitamin A Thermo Fisher 12587010 Glutamax Thermo Fisher 35- 050-061 EGF PeproTech AF-100-15 bFGF Peprotech 100-18B Cyclopamine Fisher Scientific AAJ61528MB B-27 Plus Thermo Fisher Scientific A3582801 Normocin InvivoGen ant-nr-05 BDNF PeproTech 450–02 NT3 PeproTech 450–03 StemPro Accutase TM Cell Dissociation Reagent Thermo Fisher Scientific A1110501 DNAase I Thermo Fisher Scientific EN0521 BrainPhys Neuronal Medium Stem Cell Technologies 05790 Tubastatin A Selleckchem S8049 Paraformaldehyde Electron Microscopy Sciences 19202 Fetal bovine serum Novus Biologicals S11150 (Continued on next page) 16 Cell Reports 45, 117036, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine 2000 Invitrogen 11668–019 1X RIPA Buffer Thermo Fisher Scientific 89900 Halt TM Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific 78446 IP cell lysis buffer Thermo Fisher 87787 Protease inhibitor NCM Biotech P001 Phosphatase inhibitor NCM Biotech P003 Critical commercial assays HDAC6 Fluorogenic Assay BPS Biosciences 50076–1 PureLink TM RNA Mini Kit Thermo Fisher Scientific 12183018A High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific 4368813 BCA Protein Assay Kit Thermo Fisher Scientific 23227 Deposited Data scRNAseq data NCBI GEO GEO: GSE316534 Experimental models: Cell lines Isogenic Spastin hiPSCs Generated in Lab from a control hiPSC.

    Electron Microscopy:

    Article Title: Tachykinin signaling defines distinct populations of glia in the enteric nervous system.
    Article Snippet: Article Tachykinin signaling defines distinct populations of glia in the enteric nervous system

    Article Title: Modeling spastic paraplegia 4 with corticospinal motor neuron-enriched cortical organoids reveals genotype-phenotype and HDAC6-targetable pathology.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies SOX2 R&D Systems Cat# AF2018; RRID:AB_355110 Neun Abcam Cat# ab104224; RRID:AB_10711040 MAP2 Novus Biologicals NB300-213 Cat# NB300-213; RRID:AB_2138178 βIII- Tubulin BioLegend Cat# 802001; RRID:AB_2564645 CTIP2 Abcam Cat# ab18465; RRID:AB_2064130 GFAP Abcam Cat# ab53554; RRID:AB_880202 SMI312 BioLegend Cat# 837904; RRID:AB_2566782 L1CAM BioLegend Cat# 371607; RRID:AB_2734424 vGlut1 Synaptic Systems Cat# 135 302; RRID:AB_887877 Synaptophysin Proteintech Proteintech Cat# 60191-1-Ig; RRID:AB_10915965 SMI32 BioLegend Cat# 801701; RRID:AB_2564642 Acetylated Tubulin Sigma-Aldrich Cat# T6793; RRID:AB_477585 HDAC6 Cell Signaling Technology Cat# 7612; RRID:AB_10889735 βIII- Tubulin Abcam Cat# ab7751; RRID:AB_306045 GAPDH Abcam Cat# ab8245; RRID:AB_2107448 Spastin Abcam Cat# ab77144; RRID:AB_1524436 Anti-Flag Proteintech Cat# 20543-1-AP; RRID:AB_11232216 Anti-Phosphoserine/threonine PhosphoSolutions Cat# PP2551; RRID:AB_1184778 Anti-HDAC6 Affinity Biosciences Cat# AF6485; RRID:AB_2835165 Anti-mCherry Abcam Cat# ab205402; RRID:AB_2722769 Chemicals, peptides, and recombinant proteins MTeSR TM Plus Stem Cell Technologies 100–0276 DMEM-F12 Gibco 11330032 ReLeSR TM Stem Cell Technologies 100–0483 ROCK inhibitor Y-27632 Tocris 1254 Dorsomorphin Sigma C956T21 SB431542 Tocris 1614 Neurobasal A Thermo Fisher 10-888- 022 B-27 without Vitamin A Thermo Fisher 12587010 Glutamax Thermo Fisher 35- 050-061 EGF PeproTech AF-100-15 bFGF Peprotech 100-18B Cyclopamine Fisher Scientific AAJ61528MB B-27 Plus Thermo Fisher Scientific A3582801 Normocin InvivoGen ant-nr-05 BDNF PeproTech 450–02 NT3 PeproTech 450–03 StemPro Accutase TM Cell Dissociation Reagent Thermo Fisher Scientific A1110501 DNAase I Thermo Fisher Scientific EN0521 BrainPhys Neuronal Medium Stem Cell Technologies 05790 Tubastatin A Selleckchem S8049 Paraformaldehyde Electron Microscopy Sciences 19202 Fetal bovine serum Novus Biologicals S11150 (Continued on next page) 16 Cell Reports 45, 117036, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine 2000 Invitrogen 11668–019 1X RIPA Buffer Thermo Fisher Scientific 89900 Halt TM Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific 78446 IP cell lysis buffer Thermo Fisher 87787 Protease inhibitor NCM Biotech P001 Phosphatase inhibitor NCM Biotech P003 Critical commercial assays HDAC6 Fluorogenic Assay BPS Biosciences 50076–1 PureLink TM RNA Mini Kit Thermo Fisher Scientific 12183018A High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific 4368813 BCA Protein Assay Kit Thermo Fisher Scientific 23227 Deposited Data scRNAseq data NCBI GEO GEO: GSE316534 Experimental models: Cell lines Isogenic Spastin hiPSCs Generated in Lab from a control hiPSC.

    Saline:

    Article Title: Tachykinin signaling defines distinct populations of glia in the enteric nervous system.
    Article Snippet: Article Tachykinin signaling defines distinct populations of glia in the enteric nervous system

    RNAscope:

    Article Title: Tachykinin signaling defines distinct populations of glia in the enteric nervous system.
    Article Snippet: Article Tachykinin signaling defines distinct populations of glia in the enteric nervous system

    other:

    Article Title: Modeling spastic paraplegia 4 with corticospinal motor neuron-enriched cortical organoids reveals genotype-phenotype and HDAC6-targetable pathology
    Article Snippet: MAP2 , Novus Biologicals , NB300-213 Cat# NB300-213; RRID:AB_2138178.

    Article Title: Hair metal dysregulation in Parkinson’s disease: Implications for diagnosis and gut-brain axis involvement
    Article Snippet: Anti-TH , Novus , Cat# NB300-109; RRID: AB_10077691.

    Immunocytochemistry:

    Article Title: Encephalopathy-linked UFM1 variants impede neuronal protein translation, development, and function
    Article Snippet: UFM1 (1:1000 WB) , Novus Biologicals , NBP2-94235. .. MAP2 (1:1000 ICC) , Novus Biologicals , NB300-213. .. Shank2 (1:500 IHC) , Synaptic Systems , 162 204.



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