Journal: The Lancet Regional Health - Europe

Article Title: Clinical presentation and hospitalisation risk of RSV in primary care among children younger than 5 years in Italy in four seasons between 2019 and 2023: a multicentre prospective cohort study

doi: 10.1016/j.lanepe.2026.101652

Figure Lengend Snippet: Association between onset of respiratory symptoms and RSV positivity: logistic regression analysis. Forest plot of adjusted odds ratios (ORs) with 95% confidence intervals (CIs) for T0 clinical presentation (symptoms at baseline visit) associated with pathogen groups: RSV-only, RSV with co-infections, influenza (A/B), and other respiratory pathogens. ORs were estimated via logistic regression, adjusting for age, sex, season, and geographic region. Each line represents the results of a different regression; complete results are available in . Reference category: All estimates are compared with the reference category of children who met the ARI case definition and had a valid multiplex RT-qPCR result negative for all assayed targets (“negative swab”). Other pathogens tested: adenovirus; enterovirus; parainfluenza viruses (types 1–4); human metapneumovirus; human bocavirus; rhinovirus; seasonal coronaviruses (HCoV-NL63, HCoV-229E, HCoV-OC43); SARS-CoV-2; Bordetella pertussis; Bordetella parapertussis; Chlamydophila pneumoniae; Haemophilus influenzae; Legionella pneumophila; Mycoplasma pneumoniae; Streptococcus pneumoniae . Abbreviations: RSV: respiratory syncytial virus; FLU: influenza virus; OR: odds ratio; CI: confidence intervals.

Article Snippet: Subsequently, samples were sent to a regional reference laboratory in each region, where they were tested using multiplex RT-qPCR (Allplex Respiratory Full Panel Assay, Seegene, South Korea) throughout all seasons.

Techniques: Multiplex Assay, Quantitative RT-PCR, Virus

Journal: International Journal of Women's Health

Article Title: The Impact of COVID-19 Vaccination on HR-HPV Persistence and Cervical Cytology Outcomes: A Retrospective Study

doi: 10.2147/IJWH.S594002

Figure Lengend Snippet: Patient selection and follow-up flow chart. The patient selection and clinical monitoring process are visually summarized in . Initially, 1,711 patients were screened, and 546 individuals were excluded based on Bethesda cytology classification (lesions > ASC-US) or other exclusion criteria (e.g., COVID-19, pregnancy). The study cohort consisted of 1,165 participants, divided into unvaccinated (n=573) and vaccinated (n=592) groups. During the longitudinal follow-up, the flowchart explicitly details the diagnostic interventions: a total of 436 biopsies (213 unvaccinated, 223 vaccinated) were performed during the first follow-up interval, and 185 biopsies (87 unvaccinated, 98 vaccinated) were conducted during the second follow-up.

Article Snippet: Nasopharyngeal and/or oropharyngeal samples for SARS-CoV-2 were tested using RT-PCR with two commercial kits: the BioSpeedy COVID-19 RT-qPCR Detection Kit (Bioeksen, Istanbul, Turkey), targeting the RdRp gene, and the Coronex COVID-19 Multiplex RT-qPCR Kit (DS Bio & Nano Technology, Ankara, Turkey), targeting the Orf1ab and N genes.

Techniques: Selection, Diagnostic Assay

Journal: New Microbes and New Infections

Article Title: Metagenomic surveillance reveals off-season circulation of respiratory viruses during the COVID-19 pandemic in Salvador, Brazil

doi: 10.1016/j.nmni.2026.101717

Figure Lengend Snippet: Respiratory virus identification. A) Symptomatic cases identified during the active case finding (n = 669); B) SARS-CoV-2 confirmed by single RT-qPCR and multiplex RT-qPCR and variants identified by the Whole Genomic Sequencing analysis (n = 108); C) Flu and RSV detection by the multiplex RT-qPCR (n = 32); D) Respiratory virus identified by metagenomic among participants who reported cough or fever during the visits (n = 79; and the frequencies and subcategories are included in ). EV – Enterovirus; Flu A – Influenza A virus; Flu A–C – Influenza A, B or C virus; HCoV-HKU1 – Human coronavirus HKU1; HMPV – Human metapneumovirus; HPIV – Human parainfluenza virus; KIPyV – KI polyomavirus; ORFV – Orf virus; RSV – Respiratory syncytial virus; RV – Rhinovirus; SARS-CoV-2 – Severe acute respiratory syndrome coronavirus 2; WUPyV – WU polyomavirus; HAdV – Human adenovirus; HBoV – Human bocavirus.

Article Snippet: Additionally, a multiplex RT-qPCR assay (Allplex Seegene, Seoul, South Korea) targeting Influenza A, Influenza B, RSV, and SARS-CoV-2 (3 genes) was used to validate SARS-CoV-2 results and expand detection to other respiratory viruses.

Techniques: Virus, Quantitative RT-PCR, Multiplex Assay, Genomic Sequencing