Journal: bioRxiv

Article Title: Bone marrow B cell collapse promotes bone metastasis in breast cancer

doi: 10.64898/2026.04.21.720007

Figure Lengend Snippet: (A) Schematic representation of B cell lymphopoiesis and markers that define stages of B cell development. (B, C) Representative contour plots (B) and frequency (C) of bone marrow B220 + CD19 - CD43 + CD93 + cells in C57BL/6 mice with IC injection of PBS or E0771 cells at day 17. (D, E) Representative contour plots (D) and frequency (E) of bone marrow B220 + CD19 + CD43 + CD93 + IgM - pro-B/large pre-B cells in mice described in (B). (F-I) Representative contour plots (F) and frequency of bone marrow B220 + CD19 + CD43 - IgM - IgD - small pre-B cells (G), B220 + CD19 + CD43 - IgM + IgD - immature B cells (H), and B220 + CD19 + CD43 - IgM + IgD + mature B cells (I) in mice described in (B). (J-O) Absolute numbers of B220 + CD19 + B cells (J) and frequency of bone marrow B cell subsets (K-O) in C57BL/6 mice with IC injection of PBS or E0771 cells at day 7. (P, Q) Representative pictures (P) of colony formation unit (CFU) assay of pre-B cells and quantification of CFUs (Q) from bone marrow of mice described in (B). (R) Frequency of bone marrow B cell subsets from bone marrow of mice described in (B) cultured with IL-7 for 3 days, relative to day 0. Statistical analysis was performed using unpaired two-tailed Student’s t test (C, E, G-O, Q, R). Data are represented as means ± SEM. Each dot represents a mouse in (C, E, G-O, Q, R). ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.

Article Snippet: 7.5 x 10 5 cells/ml were cultured in IMDM media containing Glutamax (Gibco, 31980030) supplemented with 10% FBS, 1 × P/S and 5μM 2-Mercaptoethanol, and 10 ng/ml of recombinant mouse IL-7 (Invitrogen, RP-8664) in a humidified incubator at 37 °C and 5% CO 2 .

Techniques: Injection, Colony-forming Unit Assay, Cell Culture, Two Tailed Test