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Exogenous hSIRT3 <t>and</t> <t>hTIMP3</t> genes exerted their downstream signaling regulation and inhibited the infiltration of inflammatory cells. a. Flowchart of short-term effects of exogenous hSIRT3 and hTIMP3 genes on myocardial injury. b. Protein expressions and quantitative analysis of CAT and <t>MnSOD</t> among the different groups. c. Protein expressions and quantitative analysis of MMP2 and MMP9 among the different groups. d. Representative images of CD11b expression in the myocardium and quantitative analysis of CD11b expression among the different groups (scale bar = 100 μm). Data are presented as the Means ± SD (n = 3). hSIRT3: human SIRT3. hTIMP3: human TIMP3. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. n.s.: not significant.
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Exogenous hSIRT3 and hTIMP3 genes exerted their downstream signaling regulation and inhibited the infiltration of inflammatory cells. a. Flowchart of short-term effects of exogenous hSIRT3 and hTIMP3 genes on myocardial injury. b. Protein expressions and quantitative analysis of CAT and MnSOD among the different groups. c. Protein expressions and quantitative analysis of MMP2 and MMP9 among the different groups. d. Representative images of CD11b expression in the myocardium and quantitative analysis of CD11b expression among the different groups (scale bar = 100 μm). Data are presented as the Means ± SD (n = 3). hSIRT3: human SIRT3. hTIMP3: human TIMP3. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. n.s.: not significant.

Journal: Materials Today Bio

Article Title: Cationic microbubble loading hSIRT3 and hTIMP3 optimize cardiac-targeted delivery and myocardial protection in the porcine MI/R model

doi: 10.1016/j.mtbio.2025.102234

Figure Lengend Snippet: Exogenous hSIRT3 and hTIMP3 genes exerted their downstream signaling regulation and inhibited the infiltration of inflammatory cells. a. Flowchart of short-term effects of exogenous hSIRT3 and hTIMP3 genes on myocardial injury. b. Protein expressions and quantitative analysis of CAT and MnSOD among the different groups. c. Protein expressions and quantitative analysis of MMP2 and MMP9 among the different groups. d. Representative images of CD11b expression in the myocardium and quantitative analysis of CD11b expression among the different groups (scale bar = 100 μm). Data are presented as the Means ± SD (n = 3). hSIRT3: human SIRT3. hTIMP3: human TIMP3. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. n.s.: not significant.

Article Snippet: The primary antibodies against hSIRT3 (1:1,000, ab217319, Abcam), hTIMP3 (1:1,000, ab276134, Abcam), SIRT3 (1:1,000, 10099-1-AP, Proteintech), TIMP3 (1:1,000, 10858-1-AP, Proteintech), MnSOD (1:5,000, 24127-1-AP, Proteintech), CAT (1:2,000, 21260-1-AP, Proteintech), MMP2 (1:500, 10373-2-AP, Proteintech), MMP9 (1:1,000, 10375-2-AP, Proteintech), caspase-3 (1:1,000, 66470-2-Ig, Proteintech), Bcl2 (1:1,000, ab32124, Abcam) and Bax (1:5,000, 50599-2-Ig, Proteintech) were incubated with the corresponding PVDF membranes overnight at 4 °C.

Techniques: Expressing