Mkate2 Emission Spectra, supplied by Evrogen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Red-Shifted Voltage Sensitive Fluorescent Proteins"
Article Title: Red-Shifted Voltage Sensitive Fluorescent Proteins
Journal: Chemistry & biology
Figure Legend Snippet: Expression and functional validation of red-shifted VSFP3.1-based voltage sensors in neuronal cultures (A–D) Confocal fluorescence imaging of primary hippocampal cultures expressing VSFP3.1_Citrine (A), VSFP3.1_TagRFP (B), VSFP3.1_mKate2 (C) or VSFP3.1_mOrange2 (D). Magnified views of boxed regions are shown in inserts. Scale bars: 40 µm (overviews) and 10 µm (inserts). (E) Optical recordings of spontaneous electrical activity in primary neuronal cultures derived from mouse hippocampus. The neurons were transfected with VSFP3.1_mOrange2 and the recordings were done under current-clamp conditions at 35°C. Asterisks indicate individual action potentials that were resolved optically in single sweeps (arrows).
Techniques Used: Expressing, Functional Assay, Fluorescence, Imaging, Activity Assay, Derivative Assay, Transfection
Figure Legend Snippet: Expression and voltage sensing characteristics of VSFP3.1 color variants in PC12 cells (A) Fluorescence and transmission images of PC12 cells expressing VSFP3.1_Cerulean, VSFP3.1_Citrine, VSFP3.1_mOrange2, VSFP3.1_TagRFP and VSFP3.1_mKate2. Scale bars: 20 µm. (B) Fluorescence signals in response to test potentials of −140 to 60 mV from a holding potential of −70 mV at 35°C
Techniques Used: Expressing, Fluorescence, Transmission Assay