Journal: ACS Omega

Article Title: Integrating Simple Microfluidics Design/Fabrication with a Novel Ionization Source for Time-Resolved Chemical Reaction Studies by Mass Spectrometry

doi: 10.1021/acsomega.5c12681

Figure Lengend Snippet: Microfluidics device design and fabrication. (A) Photograph of the microfluidics device. (B) Cartoon representation of the microfluidics device used in these studies. The general layout is shown with the 3 inlet and outlet ports labeled. The dual Herringbone structures for efficient solution mixing are also shown. (C) Device fabrication flowchart. The flowchart includes all steps for device construction as well as pretreatment for MS analysis.

Article Snippet: All three solutions were injected into the microfluidic device using two Fusion 6000 syringe pumps (Chemyx, Stafford, TX) before connecting to the cVSSI device via tubing.

Techniques: Labeling

Journal: ACS Omega

Article Title: Integrating Simple Microfluidics Design/Fabrication with a Novel Ionization Source for Time-Resolved Chemical Reaction Studies by Mass Spectrometry

doi: 10.1021/acsomega.5c12681

Figure Lengend Snippet: Total deuterium uptake of the microfluidics setup employing different total flow rates. The PTFE tubing length associated with each flow rate is provided above the respective data point. The dashed line shows the weighted m / z value of unreacted myoglobin 14+ ions. Error bars represent one standard deviation about the mean for triplicate measurements.

Article Snippet: All three solutions were injected into the microfluidic device using two Fusion 6000 syringe pumps (Chemyx, Stafford, TX) before connecting to the cVSSI device via tubing.

Techniques: Standard Deviation

Journal: Open Research Europe

Article Title: Cell motility influences microfluidics capturing in scRNA-seq

doi: 10.12688/openreseurope.19781.2

Figure Lengend Snippet: Parasites were isolated from the blood of three mice six days post-infection and purified through chromatography. Half of the parasite suspension underwent fast cooling to 0 degrees, using dry ice and ethanol (QUENCHED), while the other half remained at room temperature (RT). Both sets of samples underwent encapsulation using the Chromium controller, followed by library preparation and sequencing as recommended by 10x Genomics.

Article Snippet: In this study we confirmed that encapsulating parasites at room temperature using the 10x Genomics microfluidic device does not induce changes in motility or morphology (Supplementary Videos 1–2 extended data).

Techniques: Isolation, Infection, Purification, Chromatography, Suspension, Encapsulation, Sequencing