Journal: Advanced Science

Article Title: Mitochondrial Transplantation Augments the Reparative Capacity of Macrophages Following Myocardial Injury

doi: 10.1002/advs.202506337

Figure Lengend Snippet: MTM therapy facilitates the early differentiation and continuous infiltration of CD206+ reparative macrophages in mice following MI. A) Timeline of sample collection for Sham, MI, MI+Macro, and MI+Mito‐T‐Macro groups after MI. B) The concentration of CCL2 in serum was measured using ELISA on Days 4, 5, and 7 for each group post‐MI (n=5, mean±SEM, one‐way ANOVA, Tukey's multiple comparisons, unit: pg mL −1 ). C) The concentration of CD206 at the infarct area of myocardial tissue was measured by ELISA on Days 4, 5, and 7 for each group post‐MI (n=5, mean±SEM, one‐way ANOVA, Tukey's multiple comparisons, unit: pg mL −1 ). D) mRNA levels of genes involved in anti‐inflammatory responses (IL‐10, TGF‐β1, and VEGF‐α) and cell adhesion (TSP‐1, Mertk, and CD36) at the infarct area of myocardial tissue on Day 4 post‐MI (n=5, mean±SEM, one‐way ANOVA, Tukey's multiple comparisons). E) Representative immunofluorescence images of the myocardial injury area in each group on Day 7 post‐MI demonstrated the infiltration and distribution of CD86‐positive and CD206‐positive cells (blue: DAPI, red: CD86, green: CD206; Scale bar = 100 µm). F) Quantitative analysis of percentage of CD86‐positive cells per nuclei (left) and CD86‐positive staining (mean fluorescence intensity of CD86‐positive cells) in the myocardial injury area (right) in Figure . n = 5, mean±SEM, one‐way ANOVA, Tukey's multiple comparisons. G) Quantitative analysis of percentage of CD206‐positive cells per nuclei (left) and CD206‐positive staining (mean fluorescence intensity of CD206‐positive cells) in the myocardial injury area (right) in Figure . n = 5, mean±SEM, one‐way ANOVA, Tukey's multiple comparisons. * p < 0.05, ** p < 0.01, *** p <0.001, and **** p <0.0001.

Article Snippet: CD206 levels and chemokine CCL2 levels were measured in heart homogenates of injured myocardium and plasma obtained from different groups using ELISA kits according to the manufacturers’ instructions (ABclonal).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Fluorescence

Journal: Journal of molecular and cellular cardiology

Article Title: Mouse model of post-traumatic stress disorder negatively impacts cardiac homeostasis

doi: 10.1016/j.yjmcc.2025.01.011

Figure Lengend Snippet: A) Male PTSD-like mice had an increased trend ( p = 0.0542) in macrophages (Mac-3) in the left ventricle at 4-weeks post-IFS. No differences were observed in the female groups. Male control n = 3; Female control n = 4; Male NR n = 6; Female NR n = 7; Male PTSD-like n = 6; Female PTSD-like n = 5. B) No differences between phenotypes at 4-weeks post-IFS were observed in circulating levels of CCL2. Male control n = 4; Female control n = 4; Male NR n = 3; Female NR n = 6; Male PTSD-like n = 6; Female PTSD-like n = 4. C) Male and female NR and PTSD-like mice had decreased percent area of Ccr2 mRNA expression compared to respective controls. No significant changes were seen in the amounts of Ccr2 + CD206 mRNA colocalization but female NR mice had an increase in Ccr2 + CD86 mRNA colocalization compared to female controls at 4-weeks post-IFS. Male control n = 3; Female control n = 4; Male NR n = 5; Female NR n = 7; Male PTSD-like n = 6; Female PTSD-like n = 5. Multiple group comparisons were analyzed by two-way ANOVA with Bonferroni posttest.

Article Snippet: Assays for MMP-9 activity (Enzo, BML-AK410, 1:5 dilution of plasma, no dilution of insoluble protein fraction of the LV), circulating CCL2 levels (R&D Systems, MJE00B, 1:10 dilution), and circulating cardiac troponin I (Novus Biologicals, NBP3-00456, 1:2 dilution) were performed according to manufacturer instructions.

Techniques: Control, Expressing

Journal: The Journal of Clinical Investigation

Article Title: Induced clustering of SHP2-depleted tumor cells in vascular islands restores sensitivity to MEK/ERK inhibition

doi: 10.1172/JCI181609

Figure Lengend Snippet: ( A ) Expression levels of Il1a , ccl2 , and ccl3 in sorted populations of EGFP + B16F10 cells, EGFP – VE-cadherin + endothelial cells, and EGFP – CD45 + hematopoietic cells as measured by qPCR. The results are expressed as the relative mean ± SEM of Gapdh mRNA levels ( n = 3 experiments). ( B and C ) Representative images (scale bar: 50 μm) ( B ) showing CD45 + cell infiltrates (green, indicated by arrowheads) in control and shSHP2-silenced B16F10 tumors and quantification ( C ). Gray circles: percentage of CD45 + area/unit of tumor area; colored dots: mean/tumor ( n = 5/group). ** P < 0.01, by 2-tailed Student’s t test. ( D and E ) Representative images (scale bar: 50 μm) ( D ) showing F4/80 (green) macrophage infiltration in control and shSHP2-silenced B16F10 tumors (indicated by arrowheads) and quantification ( E ). Gray circles: percentage of F4/80 + area/unit of tumor area; colored dots: mean/tumor ( n = 5/group). ** P < 0.01, by 2-tailed Student’s t test. ( F ) Proposed model of downstream effects from SHP2 silencing in B16F10 melanoma cells leading to the formation of vascular tumor islands and surrounding tumor cell death. Results in panels A , C and E are presented as means± SEM.

Article Snippet: Tumor CCL2 levels were measured with the Mouse CCL2 DuoSet ELISA kit (R&D Systems, mLMJE00B), and the colorimetric reaction was measured at 450 nm using a microplate reader (Imgen Technologies, BMG LABTECH).

Techniques: Expressing, Control