Journal: Acta Pharmaceutica Sinica. B

Article Title: Neutralizing dentin sialophosphoprotein facilitates tumor vascular normalization in colorectal cancer by blocking the crosstalk between tumor cells and endothelial cells

doi: 10.1016/j.apsb.2026.06.011

Figure Lengend Snippet: DSPP/ α v β 3/MAPK signaling axis promotes tumor vessel abnormalization. (A, B) Investigating the potential regulatory functions of DSPP in CRC by the WiKi (A) and KEGG (B) pathway enrichment analysis. (C) The mitogen-activated protein kinase (MAPK)-related pathways were enriched in CRC with high DSPP expression by GSEA analysis. (D) Western blot assays showing the expression of the FAK/ERK pathway components in HUVEC treated with CM from the indicated CRC cells (E, F) Representative tubular structures formed by HUVECs treated with indicated CM from indicated HCT8/Caco-2 cells. Data are presented as mean ± SD ( n = 3). (G) Heat map demonstrates the differentially expressed genes related to angiogenesis and matrix metalloproteinase family in HUVEC treated with CM from control and sg DSPP HCT116 cells. (H) Detecting the effect of AZD6244 on the DSPP-upregulated expression of angiogenesis and matrix metalloproteinase family-related genes by real-time quantitative PCR. Data are presented as mean ± SD ( n = 4). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: Inhibitors, including the mitogen-activated protein kinase (MAPK) kinase inhibitor selumetinib (AZD6244, MCE, Monmouth Junction, NJ, USA) or cyclo (-RGDfK) TFA (HY-P0023A, MCE), were introduced into the cultured cells.

Techniques: Expressing, Western Blot, Control, Real-time Polymerase Chain Reaction

Journal: Acta Pharmaceutica Sinica. B

Article Title: Neutralizing dentin sialophosphoprotein facilitates tumor vascular normalization in colorectal cancer by blocking the crosstalk between tumor cells and endothelial cells

doi: 10.1016/j.apsb.2026.06.011

Figure Lengend Snippet: A schematic diagram shows the action mechanism of DSPP in regulating tumor vascular abnormalization to induce tumor metastasis. In the tumor microenvironment, IL-7F enhances the expression and secretion of DSPP in CRC. The CRC-secreted DSPP binds to the integrin α v β 3 on the endothelial cell surface and activates the downstream FAK/MAPK signaling to induce tumor vascular abnormalization, finally promote tumor metastasis.

Article Snippet: Inhibitors, including the mitogen-activated protein kinase (MAPK) kinase inhibitor selumetinib (AZD6244, MCE, Monmouth Junction, NJ, USA) or cyclo (-RGDfK) TFA (HY-P0023A, MCE), were introduced into the cultured cells.

Techniques: Expressing

Journal: Poultry Science

Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens

doi: 10.1016/j.psj.2026.106914

Figure Lengend Snippet: TLCA regulates mitochondrial biogenesis and altered myofiber type composition through the p38 MAPK/PGC-1α signaling pathway. A Cell viability of cells treated with different concentrations of TLCA for 24 h (n = 10). B & F The mRNA expression of MYH1A and MYH7B (n = 6). C & G Intracellular enzymatic activities of succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH), ATP content and mitochondrial DNA (mtDNA) content (n = 6). D & H The mRNA expression of HK1, GPI, PGAM1, PGK1, PYGL, PGC-1α, NRF1 and TFAM (n = 6). E The protein expression of PGC-1α, p38 MAPK and phosphorylation p38 MAPK (n = 3). I The protein expression of PGC-1α (n = 3). Data were shown as mean ± SD; *Represents significant difference when compared with 0 μM TLCA or CON group, and # represents significant difference when compared with TLCA group. *& # P < 0.05, ⁎⁎ & ## P < 0.01, ⁎⁎⁎ & ### P < 0.001.

Article Snippet: The differentiation medium containing TLCA and p38 MAPK inhibitor SB203580 (152121-47-6, MedChemExpress, Shanghai, China) was added to CPMs after 4 days of differentiation, and the cells were collected after 24 h.

Techniques: Expressing, Phospho-proteomics