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Mouse Anti 53bp1 #Mab3802, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Anti 53bp1 Mab3802, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibodies for 53bp1 mab3802  (Millipore)
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Assessment of DNA damage in meningioma spheroids after EBRT and PRRT. ( A , D ) IHC staining for SSTR2 in parental tumor tissue for a meningioma staining strongly (MO-14, ( A ) and weakly (MO-12 ( D )) SSTR2-positive. ( B , E ) Representative images of <t>53BP1</t> IF staining (red) used for the quantifications in ( C , F ). Examples are shown for EBRT (2 Gy)- and PRRT (1 MBq/mL)-treated spheroids at both day 0 and day 2 post-therapy. DAPI (blue) was used as a nuclear counterstain. ( C , F ) Quantification of the number of 53BP1 foci per nucleus after EBRT, PRRT, or PRRT co-incubated with DOTA-TATE at day 0 or day 2 post-therapy for MO-14 ( C ) and MO-12 ( F ). Black horizontal bars indicate the mean. ns = not significant; *** p ≤ 0.001; **** p ≤ 0.0001. Line graphs on the right show the mean number of 53BP1 foci for each condition. Quantification for other meningioma samples can be found in .
Primary Antibodies For 53bp1 Mab3802, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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γh2ax(s139) mab3802  (Millipore)
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Assessment of DNA damage in meningioma spheroids after EBRT and PRRT. ( A , D ) IHC staining for SSTR2 in parental tumor tissue for a meningioma staining strongly (MO-14, ( A ) and weakly (MO-12 ( D )) SSTR2-positive. ( B , E ) Representative images of <t>53BP1</t> IF staining (red) used for the quantifications in ( C , F ). Examples are shown for EBRT (2 Gy)- and PRRT (1 MBq/mL)-treated spheroids at both day 0 and day 2 post-therapy. DAPI (blue) was used as a nuclear counterstain. ( C , F ) Quantification of the number of 53BP1 foci per nucleus after EBRT, PRRT, or PRRT co-incubated with DOTA-TATE at day 0 or day 2 post-therapy for MO-14 ( C ) and MO-12 ( F ). Black horizontal bars indicate the mean. ns = not significant; *** p ≤ 0.001; **** p ≤ 0.0001. Line graphs on the right show the mean number of 53BP1 foci for each condition. Quantification for other meningioma samples can be found in .
γh2ax(s139) Mab3802, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-53bp1/mab3802  (Millipore)
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Assessment of DNA damage in meningioma spheroids after EBRT and PRRT. ( A , D ) IHC staining for SSTR2 in parental tumor tissue for a meningioma staining strongly (MO-14, ( A ) and weakly (MO-12 ( D )) SSTR2-positive. ( B , E ) Representative images of <t>53BP1</t> IF staining (red) used for the quantifications in ( C , F ). Examples are shown for EBRT (2 Gy)- and PRRT (1 MBq/mL)-treated spheroids at both day 0 and day 2 post-therapy. DAPI (blue) was used as a nuclear counterstain. ( C , F ) Quantification of the number of 53BP1 foci per nucleus after EBRT, PRRT, or PRRT co-incubated with DOTA-TATE at day 0 or day 2 post-therapy for MO-14 ( C ) and MO-12 ( F ). Black horizontal bars indicate the mean. ns = not significant; *** p ≤ 0.001; **** p ≤ 0.0001. Line graphs on the right show the mean number of 53BP1 foci for each condition. Quantification for other meningioma samples can be found in .
Anti 53bp1/Mab3802, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Assessment of DNA damage in meningioma spheroids after EBRT and PRRT. ( A , D ) IHC staining for SSTR2 in parental tumor tissue for a meningioma staining strongly (MO-14, ( A ) and weakly (MO-12 ( D )) SSTR2-positive. ( B , E ) Representative images of <t>53BP1</t> IF staining (red) used for the quantifications in ( C , F ). Examples are shown for EBRT (2 Gy)- and PRRT (1 MBq/mL)-treated spheroids at both day 0 and day 2 post-therapy. DAPI (blue) was used as a nuclear counterstain. ( C , F ) Quantification of the number of 53BP1 foci per nucleus after EBRT, PRRT, or PRRT co-incubated with DOTA-TATE at day 0 or day 2 post-therapy for MO-14 ( C ) and MO-12 ( F ). Black horizontal bars indicate the mean. ns = not significant; *** p ≤ 0.001; **** p ≤ 0.0001. Line graphs on the right show the mean number of 53BP1 foci for each condition. Quantification for other meningioma samples can be found in .
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53bp1 mab3802 antibody  (Millipore)
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Millipore 53bp1 mab3802 antibody
Assessment of DNA damage in meningioma spheroids after EBRT and PRRT. ( A , D ) IHC staining for SSTR2 in parental tumor tissue for a meningioma staining strongly (MO-14, ( A ) and weakly (MO-12 ( D )) SSTR2-positive. ( B , E ) Representative images of <t>53BP1</t> IF staining (red) used for the quantifications in ( C , F ). Examples are shown for EBRT (2 Gy)- and PRRT (1 MBq/mL)-treated spheroids at both day 0 and day 2 post-therapy. DAPI (blue) was used as a nuclear counterstain. ( C , F ) Quantification of the number of 53BP1 foci per nucleus after EBRT, PRRT, or PRRT co-incubated with DOTA-TATE at day 0 or day 2 post-therapy for MO-14 ( C ) and MO-12 ( F ). Black horizontal bars indicate the mean. ns = not significant; *** p ≤ 0.001; **** p ≤ 0.0001. Line graphs on the right show the mean number of 53BP1 foci for each condition. Quantification for other meningioma samples can be found in .
53bp1 Mab3802 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Assessment of DNA damage in meningioma spheroids after EBRT and PRRT. ( A , D ) IHC staining for SSTR2 in parental tumor tissue for a meningioma staining strongly (MO-14, ( A ) and weakly (MO-12 ( D )) SSTR2-positive. ( B , E ) Representative images of 53BP1 IF staining (red) used for the quantifications in ( C , F ). Examples are shown for EBRT (2 Gy)- and PRRT (1 MBq/mL)-treated spheroids at both day 0 and day 2 post-therapy. DAPI (blue) was used as a nuclear counterstain. ( C , F ) Quantification of the number of 53BP1 foci per nucleus after EBRT, PRRT, or PRRT co-incubated with DOTA-TATE at day 0 or day 2 post-therapy for MO-14 ( C ) and MO-12 ( F ). Black horizontal bars indicate the mean. ns = not significant; *** p ≤ 0.001; **** p ≤ 0.0001. Line graphs on the right show the mean number of 53BP1 foci for each condition. Quantification for other meningioma samples can be found in .

Journal: Cancers

Article Title: Investigating the Radiobiological Response to Peptide Receptor Radionuclide Therapy Using Patient-Derived Meningioma Spheroids

doi: 10.3390/cancers16142515

Figure Lengend Snippet: Assessment of DNA damage in meningioma spheroids after EBRT and PRRT. ( A , D ) IHC staining for SSTR2 in parental tumor tissue for a meningioma staining strongly (MO-14, ( A ) and weakly (MO-12 ( D )) SSTR2-positive. ( B , E ) Representative images of 53BP1 IF staining (red) used for the quantifications in ( C , F ). Examples are shown for EBRT (2 Gy)- and PRRT (1 MBq/mL)-treated spheroids at both day 0 and day 2 post-therapy. DAPI (blue) was used as a nuclear counterstain. ( C , F ) Quantification of the number of 53BP1 foci per nucleus after EBRT, PRRT, or PRRT co-incubated with DOTA-TATE at day 0 or day 2 post-therapy for MO-14 ( C ) and MO-12 ( F ). Black horizontal bars indicate the mean. ns = not significant; *** p ≤ 0.001; **** p ≤ 0.0001. Line graphs on the right show the mean number of 53BP1 foci for each condition. Quantification for other meningioma samples can be found in .

Article Snippet: For IF and IHC stainings, primary antibodies for SSTR2 (Abcam, Cambridge, UK; ab134152, 8.15 μg/mL), 53BP1 (Millipore, Amsterdam, The Netherlands; MAB3802, 1 μg/mL), and Ki-67 (DAKO M7240, clone MIB1, 0.48 μg/mL) were used.

Techniques: Immunohistochemistry, Staining, Incubation