Journal: Drug Testing and Analysis

Article Title: Improvement of EPO Transgene Detection From Polymeric Dried Blood Spots for Antidoping Application

doi: 10.1002/dta.70008

Figure Lengend Snippet: Validation of RM‐EPO detection from TASSO DBS. A. Selectivity, sensitivity (Limit of detection) and reproducibility of detection are shown. PCR was performed using Taqman EPO assay on DNA extracted from DBS TASSO M‐20 loaded with 10 different blood samples spiked at 0–1500–2500–5000 c/mL RM‐EPO. Cq values are indicated. Examples of size of amplified products are shown for bloods spiked at 5000 c/mL. Reproducibility experiments were performed after DNA extraction from the second and third spot of the TASSO device. B. CP performed on the fourth spot of TASSO M‐20 (“B analysis”). PCR was performed using NMI CP on DNA extracted from the fourth spot (last spot) of DBS TASSO M‐20 loaded with blood samples spiked at 5000 c/mL RM‐EPO. Cq values are indicated. Examples of size of amplified products are shown.

Article Snippet: In addition to the cellulosic paper (Whatman Protein 903 Saver card) and volumetric polymer device (20‐μL Mitra, Neoteryx) that usually require finger pricks to collect blood, we evaluated another quantitative device TASSO M‐20 (Tasso Inc.) that presents the advantage of collecting capillary blood directly from the upper arm to directly fill four polymeric spots that absorb a similar volume of blood.

Techniques: Biomarker Discovery, Amplification, DNA Extraction