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Pentablock copolymer (PBC) micelles enhanced antibody titers in C57BL/6 mice 2- and 4-weeks postimmunization. Serum collected at 14 and 28 dpi were used to evaluate anti-OVA total IgG titers using ELISA. Statistical differences between the treatment groups within each time-point were determined using one-way <t>ANOVA</t> <t>and</t> <t>Tukey-post</t> t test on the log 2 transforms of the titer dilution values. * indicates p < 0.05 and **** indicates p < 0.0001. Data are representative of two independent experiments with C57BL/6 mice and one independent experiment with BALB/c mice with 4–5 animals in each experiment per treatment group.
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Pentablock copolymer (PBC) micelles enhanced antibody titers in C57BL/6 mice 2- and 4-weeks postimmunization. Serum collected at 14 and 28 dpi were used to evaluate anti-OVA total IgG titers using ELISA. Statistical differences between the treatment groups within each time-point were determined using one-way ANOVA and Tukey-post t test on the log 2 transforms of the titer dilution values. * indicates p < 0.05 and **** indicates p < 0.0001. Data are representative of two independent experiments with C57BL/6 mice and one independent experiment with BALB/c mice with 4–5 animals in each experiment per treatment group.

Journal: ACS biomaterials science & engineering

Article Title: Pentablock Copolymer Micelle Nanoadjuvants Enhance Cytosolic Delivery of Antigen and Improve Vaccine Efficacy while Inducing Low Inflammation

doi: 10.1021/acsbiomaterials.8b01591

Figure Lengend Snippet: Pentablock copolymer (PBC) micelles enhanced antibody titers in C57BL/6 mice 2- and 4-weeks postimmunization. Serum collected at 14 and 28 dpi were used to evaluate anti-OVA total IgG titers using ELISA. Statistical differences between the treatment groups within each time-point were determined using one-way ANOVA and Tukey-post t test on the log 2 transforms of the titer dilution values. * indicates p < 0.05 and **** indicates p < 0.0001. Data are representative of two independent experiments with C57BL/6 mice and one independent experiment with BALB/c mice with 4–5 animals in each experiment per treatment group.

Article Snippet: For the antibody titer data, statistical significance was determined using one-way ANOVA and Tukey-post t test on the log 2 transformed titers using GraphPad (Prism 7.0, GraphPad Software, La Jolla, CA).

Techniques: Enzyme-linked Immunosorbent Assay