Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Structure-guided discovery of novel ERα-binding peptides with in vitro antiproliferative activity

doi: 10.1080/14756366.2026.2713724

Figure Lengend Snippet: In vitro antiproliferative activity of Peptides 1–4 and control peptides in SK-UT-1 and HUtSMC cells after 48 h of treatment.

Article Snippet: The human uterine leiomyosarcoma cell line SK-UT-1 (HTB-114), primary human uterine smooth muscle cells (HuTSMCs; PCS-460–011), human umbilical vein endothelial cells (HUVECs; PCS-100–010), human adult dermal fibroblasts (HDFa; PCS-201–012), and HK-2 cells (CRL-2190) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: In Vitro, Activity Assay, Control

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Structure-guided discovery of novel ERα-binding peptides with in vitro antiproliferative activity

doi: 10.1080/14756366.2026.2713724

Figure Lengend Snippet: Five-day in vitro antiproliferative assessment of Peptide-4 and control peptides in SK-UT-1 and tested normal human cell lines.

Article Snippet: The human uterine leiomyosarcoma cell line SK-UT-1 (HTB-114), primary human uterine smooth muscle cells (HuTSMCs; PCS-460–011), human umbilical vein endothelial cells (HUVECs; PCS-100–010), human adult dermal fibroblasts (HDFa; PCS-201–012), and HK-2 cells (CRL-2190) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: In Vitro, Control

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Structure-guided discovery of novel ERα-binding peptides with in vitro antiproliferative activity

doi: 10.1080/14756366.2026.2713724

Figure Lengend Snippet: ERα knockdown attenuates the antiproliferative activity of Peptide-4 in SK-UT-1 cells.

Article Snippet: The human uterine leiomyosarcoma cell line SK-UT-1 (HTB-114), primary human uterine smooth muscle cells (HuTSMCs; PCS-460–011), human umbilical vein endothelial cells (HUVECs; PCS-100–010), human adult dermal fibroblasts (HDFa; PCS-201–012), and HK-2 cells (CRL-2190) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Knockdown, Activity Assay

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Structure-guided discovery of novel ERα-binding peptides with in vitro antiproliferative activity

doi: 10.1080/14756366.2026.2713724

Figure Lengend Snippet: Effects of Peptide-4 and anti-miR-149G on c-Myc and Cyclin D1 mRNA expression in SK-UT-1 cells. (A) Peptide-4 treatment decreased the relative mRNA levels of c-Myc and Cyclin D1 at 1 and 5 μM, with a greater reduction observed at 5 μM. (B) Anti-miR-149G treatment produced a similar concentration-associated decrease in c-Myc and Cyclin D1 mRNA expression. mRNA levels were determined by RT-qPCR and normalised to the control group. Data are presented as mean ± SD ( n = 3). *** P < 0.001 vs control. Corresponding Hedges’ g standardised effect sizes and their 95% confidence intervals are summarised in Table S3 .

Article Snippet: The human uterine leiomyosarcoma cell line SK-UT-1 (HTB-114), primary human uterine smooth muscle cells (HuTSMCs; PCS-460–011), human umbilical vein endothelial cells (HUVECs; PCS-100–010), human adult dermal fibroblasts (HDFa; PCS-201–012), and HK-2 cells (CRL-2190) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Produced, Concentration Assay, Quantitative RT-PCR, Control

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Structure-guided discovery of novel ERα-binding peptides with in vitro antiproliferative activity

doi: 10.1080/14756366.2026.2713724

Figure Lengend Snippet: Effect of Peptide-4, anti-miR-149G, and their combination on SK-UT-1 cell inhibition. Peptide-4 and anti-miR-149G alone showed moderate inhibitory effects on SK-UT-1 cells, while the combined treatment resulted in a higher inhibition rate than either single treatment. The dashed line indicates the 60% inhibition reference level. Cell inhibition rates were determined by the MTT assay. Data are presented as mean ± SD ( n = 3). *** P < 0.001 vs either single-treatment group. Corresponding Hedges’ g standardised effect sizes and their 95% confidence intervals are summarised in Table S4 .

Article Snippet: The human uterine leiomyosarcoma cell line SK-UT-1 (HTB-114), primary human uterine smooth muscle cells (HuTSMCs; PCS-460–011), human umbilical vein endothelial cells (HUVECs; PCS-100–010), human adult dermal fibroblasts (HDFa; PCS-201–012), and HK-2 cells (CRL-2190) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Inhibition, MTT Assay