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Inhibitory effects of tilapia skin collagen peptides on TYR activity in B16F10 cells. TYR activity was determined by measuring dopachrome formation <t>from</t> <t>L-dopa</t> and normalized to total protein. ( A ) Concentration-dependent inhibition after 48 h of treatment at concentrations of 0.1–2.0 mg/mL. ( B ) Time-dependent inhibition by PFRMY (1.0 mg/mL) over 24, 48, and 72 h. ( C ) Colorimetric changes in L-dopa solution following incubation with cell lysates from B16F10 cells treated with different <t>samples.</t> <t>α-MSH-treated</t> cells served as the control. Data are presented as mean ± SD ( n = 3). (* p < 0.05, ** p < 0.01, and *** p < 0.001).
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Inhibitory effects of tilapia skin collagen peptides on TYR activity in B16F10 cells. TYR activity was determined by measuring dopachrome formation <t>from</t> <t>L-dopa</t> and normalized to total protein. ( A ) Concentration-dependent inhibition after 48 h of treatment at concentrations of 0.1–2.0 mg/mL. ( B ) Time-dependent inhibition by PFRMY (1.0 mg/mL) over 24, 48, and 72 h. ( C ) Colorimetric changes in L-dopa solution following incubation with cell lysates from B16F10 cells treated with different <t>samples.</t> <t>α-MSH-treated</t> cells served as the control. Data are presented as mean ± SD ( n = 3). (* p < 0.05, ** p < 0.01, and *** p < 0.001).
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Inhibitory effects of tilapia skin collagen peptides on TYR activity in B16F10 cells. TYR activity was determined by measuring dopachrome formation <t>from</t> <t>L-dopa</t> and normalized to total protein. ( A ) Concentration-dependent inhibition after 48 h of treatment at concentrations of 0.1–2.0 mg/mL. ( B ) Time-dependent inhibition by PFRMY (1.0 mg/mL) over 24, 48, and 72 h. ( C ) Colorimetric changes in L-dopa solution following incubation with cell lysates from B16F10 cells treated with different <t>samples.</t> <t>α-MSH-treated</t> cells served as the control. Data are presented as mean ± SD ( n = 3). (* p < 0.05, ** p < 0.01, and *** p < 0.001).
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Inhibitory effects of tilapia skin collagen peptides on TYR activity in B16F10 cells. TYR activity was determined by measuring dopachrome formation <t>from</t> <t>L-dopa</t> and normalized to total protein. ( A ) Concentration-dependent inhibition after 48 h of treatment at concentrations of 0.1–2.0 mg/mL. ( B ) Time-dependent inhibition by PFRMY (1.0 mg/mL) over 24, 48, and 72 h. ( C ) Colorimetric changes in L-dopa solution following incubation with cell lysates from B16F10 cells treated with different <t>samples.</t> <t>α-MSH-treated</t> cells served as the control. Data are presented as mean ± SD ( n = 3). (* p < 0.05, ** p < 0.01, and *** p < 0.001).
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MedChemExpress l dopa buffer
Inhibitory effects of tilapia skin collagen peptides on TYR activity in B16F10 cells. TYR activity was determined by measuring dopachrome formation <t>from</t> <t>L-dopa</t> and normalized to total protein. ( A ) Concentration-dependent inhibition after 48 h of treatment at concentrations of 0.1–2.0 mg/mL. ( B ) Time-dependent inhibition by PFRMY (1.0 mg/mL) over 24, 48, and 72 h. ( C ) Colorimetric changes in L-dopa solution following incubation with cell lysates from B16F10 cells treated with different <t>samples.</t> <t>α-MSH-treated</t> cells served as the control. Data are presented as mean ± SD ( n = 3). (* p < 0.05, ** p < 0.01, and *** p < 0.001).
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Inhibitory effects of tilapia skin collagen peptides on TYR activity in B16F10 cells. TYR activity was determined by measuring dopachrome formation from L-dopa and normalized to total protein. ( A ) Concentration-dependent inhibition after 48 h of treatment at concentrations of 0.1–2.0 mg/mL. ( B ) Time-dependent inhibition by PFRMY (1.0 mg/mL) over 24, 48, and 72 h. ( C ) Colorimetric changes in L-dopa solution following incubation with cell lysates from B16F10 cells treated with different samples. α-MSH-treated cells served as the control. Data are presented as mean ± SD ( n = 3). (* p < 0.05, ** p < 0.01, and *** p < 0.001).

Journal: Foods

Article Title: Tilapia Skin-Derived Peptide PFRMY Attenuates Melanogenesis and Tyrosinase Activity via α-MSH/PKA/CREB Signaling Pathways in B16F10 Murine Melanoma Cells

doi: 10.3390/foods15081378

Figure Lengend Snippet: Inhibitory effects of tilapia skin collagen peptides on TYR activity in B16F10 cells. TYR activity was determined by measuring dopachrome formation from L-dopa and normalized to total protein. ( A ) Concentration-dependent inhibition after 48 h of treatment at concentrations of 0.1–2.0 mg/mL. ( B ) Time-dependent inhibition by PFRMY (1.0 mg/mL) over 24, 48, and 72 h. ( C ) Colorimetric changes in L-dopa solution following incubation with cell lysates from B16F10 cells treated with different samples. α-MSH-treated cells served as the control. Data are presented as mean ± SD ( n = 3). (* p < 0.05, ** p < 0.01, and *** p < 0.001).

Article Snippet: L-dopa and α-arbutin were purchased from Macklin Co., Ltd. (Beijing, China).

Techniques: Activity Assay, Concentration Assay, Inhibition, Incubation, Control