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Expression of apoptosis-related proteins and genes in MOLT-3 cells after RNA interference of EIF2AK3 gene. ( A ) Expression of Bax at translational and transcriptional levels. ( B ) Bcl-2 protein and gene expression. ( C ) Relative expression levels of CASP3 gene, total and cleaved forms of caspase-3. ( D ) Protein content of transcription factor CHOP and protein kinase <t>JNK1/2/3.</t> Shown are typical immunoblots for one sample from each group, average data for the levels of studied proteins (n = 6, one-way ANOVA, Dunnett test), and average values for gene expression (n = 8, Kruskal–Wallis criterion, Dunn test). Cont—control without transfection, si-PERK—RNA interference of the EIF2AK3 gene, si-Cont—transfection with control siRNA. ** p < 0.01, *** p < 0.001 compared to control; # p < 0.05, ## p < 0.01, ### p < 0.001 compared to control transfection.
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Expression of apoptosis-related proteins and genes in MOLT-3 cells after RNA interference of EIF2AK3 gene. ( A ) Expression of Bax at translational and transcriptional levels. ( B ) Bcl-2 protein and gene expression. ( C ) Relative expression levels of CASP3 gene, total and cleaved forms of caspase-3. ( D ) Protein content of transcription factor CHOP and protein kinase <t>JNK1/2/3.</t> Shown are typical immunoblots for one sample from each group, average data for the levels of studied proteins (n = 6, one-way ANOVA, Dunnett test), and average values for gene expression (n = 8, Kruskal–Wallis criterion, Dunn test). Cont—control without transfection, si-PERK—RNA interference of the EIF2AK3 gene, si-Cont—transfection with control siRNA. ** p < 0.01, *** p < 0.001 compared to control; # p < 0.05, ## p < 0.01, ### p < 0.001 compared to control transfection.
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Expression of apoptosis-related proteins and genes in MOLT-3 cells after RNA interference of EIF2AK3 gene. ( A ) Expression of Bax at translational and transcriptional levels. ( B ) Bcl-2 protein and gene expression. ( C ) Relative expression levels of CASP3 gene, total and cleaved forms of caspase-3. ( D ) Protein content of transcription factor CHOP and protein kinase <t>JNK1/2/3.</t> Shown are typical immunoblots for one sample from each group, average data for the levels of studied proteins (n = 6, one-way ANOVA, Dunnett test), and average values for gene expression (n = 8, Kruskal–Wallis criterion, Dunn test). Cont—control without transfection, si-PERK—RNA interference of the EIF2AK3 gene, si-Cont—transfection with control siRNA. ** p < 0.01, *** p < 0.001 compared to control; # p < 0.05, ## p < 0.01, ### p < 0.001 compared to control transfection.
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Expression of apoptosis-related proteins and genes in MOLT-3 cells after RNA interference of EIF2AK3 gene. ( A ) Expression of Bax at translational and transcriptional levels. ( B ) Bcl-2 protein and gene expression. ( C ) Relative expression levels of CASP3 gene, total and cleaved forms of caspase-3. ( D ) Protein content of transcription factor CHOP and protein kinase <t>JNK1/2/3.</t> Shown are typical immunoblots for one sample from each group, average data for the levels of studied proteins (n = 6, one-way ANOVA, Dunnett test), and average values for gene expression (n = 8, Kruskal–Wallis criterion, Dunn test). Cont—control without transfection, si-PERK—RNA interference of the EIF2AK3 gene, si-Cont—transfection with control siRNA. ** p < 0.01, *** p < 0.001 compared to control; # p < 0.05, ## p < 0.01, ### p < 0.001 compared to control transfection.
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Expression of apoptosis-related proteins and genes in MOLT-3 cells after RNA interference of EIF2AK3 gene. ( A ) Expression of Bax at translational and transcriptional levels. ( B ) Bcl-2 protein and gene expression. ( C ) Relative expression levels of CASP3 gene, total and cleaved forms of caspase-3. ( D ) Protein content of transcription factor CHOP and protein kinase <t>JNK1/2/3.</t> Shown are typical immunoblots for one sample from each group, average data for the levels of studied proteins (n = 6, one-way ANOVA, Dunnett test), and average values for gene expression (n = 8, Kruskal–Wallis criterion, Dunn test). Cont—control without transfection, si-PERK—RNA interference of the EIF2AK3 gene, si-Cont—transfection with control siRNA. ** p < 0.01, *** p < 0.001 compared to control; # p < 0.05, ## p < 0.01, ### p < 0.001 compared to control transfection.
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Expression of apoptosis-related proteins and genes in MOLT-3 cells after RNA interference of EIF2AK3 gene. ( A ) Expression of Bax at translational and transcriptional levels. ( B ) Bcl-2 protein and gene expression. ( C ) Relative expression levels of CASP3 gene, total and cleaved forms of caspase-3. ( D ) Protein content of transcription factor CHOP and protein kinase JNK1/2/3. Shown are typical immunoblots for one sample from each group, average data for the levels of studied proteins (n = 6, one-way ANOVA, Dunnett test), and average values for gene expression (n = 8, Kruskal–Wallis criterion, Dunn test). Cont—control without transfection, si-PERK—RNA interference of the EIF2AK3 gene, si-Cont—transfection with control siRNA. ** p < 0.01, *** p < 0.001 compared to control; # p < 0.05, ## p < 0.01, ### p < 0.001 compared to control transfection.

Journal: International Journal of Molecular Sciences

Article Title: Differential Effects of PERK and IRE1α Silencing on Expression of Apoptosis and Autophagy Markers in T-Lymphoblastic Leukemia MOLT-3 Cells

doi: 10.3390/ijms27156588

Figure Lengend Snippet: Expression of apoptosis-related proteins and genes in MOLT-3 cells after RNA interference of EIF2AK3 gene. ( A ) Expression of Bax at translational and transcriptional levels. ( B ) Bcl-2 protein and gene expression. ( C ) Relative expression levels of CASP3 gene, total and cleaved forms of caspase-3. ( D ) Protein content of transcription factor CHOP and protein kinase JNK1/2/3. Shown are typical immunoblots for one sample from each group, average data for the levels of studied proteins (n = 6, one-way ANOVA, Dunnett test), and average values for gene expression (n = 8, Kruskal–Wallis criterion, Dunn test). Cont—control without transfection, si-PERK—RNA interference of the EIF2AK3 gene, si-Cont—transfection with control siRNA. ** p < 0.01, *** p < 0.001 compared to control; # p < 0.05, ## p < 0.01, ### p < 0.001 compared to control transfection.

Article Snippet: JNK1/2/3 , a/rabbit , 1:1000 , AbClonal , A18678.

Techniques: Expressing, Gene Expression, Western Blot, Control, Transfection

The effect of RNA interference of ERN1 gene on the expression of apoptosis-related proteins in MOLT-3 cells. ( A ) Expression of Bax at the translational and transcriptional levels. ( B ) Bcl-2 protein and gene expression. ( C ) Relative expression levels of CASP3 gene, total and cleaved forms of caspase-3. ( D ) Cellular levels of transcription factor CHOP and protein kinase JNK1/2/3. Shown are typical immunoblots for one sample from each group, average data for the levels of studied proteins (n = 6, one-way ANOVA, Dunnett test), and average values for gene expression (n = 8, Kruskal–Wallis criterion, Dunn test). Cont—control without transfection, si-IRE1—RNA interference of ERN1 gene, si-Cont—transfection with control RNA. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to control; ## p < 0.01, ### p < 0.001 compared to the control transfection.

Journal: International Journal of Molecular Sciences

Article Title: Differential Effects of PERK and IRE1α Silencing on Expression of Apoptosis and Autophagy Markers in T-Lymphoblastic Leukemia MOLT-3 Cells

doi: 10.3390/ijms27156588

Figure Lengend Snippet: The effect of RNA interference of ERN1 gene on the expression of apoptosis-related proteins in MOLT-3 cells. ( A ) Expression of Bax at the translational and transcriptional levels. ( B ) Bcl-2 protein and gene expression. ( C ) Relative expression levels of CASP3 gene, total and cleaved forms of caspase-3. ( D ) Cellular levels of transcription factor CHOP and protein kinase JNK1/2/3. Shown are typical immunoblots for one sample from each group, average data for the levels of studied proteins (n = 6, one-way ANOVA, Dunnett test), and average values for gene expression (n = 8, Kruskal–Wallis criterion, Dunn test). Cont—control without transfection, si-IRE1—RNA interference of ERN1 gene, si-Cont—transfection with control RNA. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to control; ## p < 0.01, ### p < 0.001 compared to the control transfection.

Article Snippet: JNK1/2/3 , a/rabbit , 1:1000 , AbClonal , A18678.

Techniques: Expressing, Gene Expression, Western Blot, Control, Transfection