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Microcolonies architecture in GSFM (▴) or S-GSFM (●), after 96h at 25°C. A : Macroscopic images are obtained using an automated robot that integrates incubation and high-resolution imaging. Microscopic observations of microcolonies, endpoint stained with the cell-permeant nucleic acid dye Syto 61, are obtained with confocal laser scanning microscopy. Representative pictures correspond to the microcolonies <t>isosurface</t> after fluorescence segmentation using the Imaris isosurface functions. B : Quantitative parameters extracted from the isosurface of microcolonies in Imaris software. Each point represents an independent replicate (n=9), bars correspond to the mean of volume of microcolonies log 10 (µm 3 ) and sphericity (A.U.). A two-way ANOVA was performed to assess the effect of food stress. When a significant effect of the environment was detected, the Bonferroni multiple comparisons test was applied to evaluate the level of significance. Asterisks indicate significant differences between environments (p<0.05).
Imaris Isosurface Function, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Microcolonies architecture in GSFM (▴) or S-GSFM (●), after 96h at 25°C. A : Macroscopic images are obtained using an automated robot that integrates incubation and high-resolution imaging. Microscopic observations of microcolonies, endpoint stained with the cell-permeant nucleic acid dye Syto 61, are obtained with confocal laser scanning microscopy. Representative pictures correspond to the microcolonies <t>isosurface</t> after fluorescence segmentation using the Imaris isosurface functions. B : Quantitative parameters extracted from the isosurface of microcolonies in Imaris software. Each point represents an independent replicate (n=9), bars correspond to the mean of volume of microcolonies log 10 (µm 3 ) and sphericity (A.U.). A two-way ANOVA was performed to assess the effect of food stress. When a significant effect of the environment was detected, the Bonferroni multiple comparisons test was applied to evaluate the level of significance. Asterisks indicate significant differences between environments (p<0.05).
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Microcolonies architecture in GSFM (▴) or S-GSFM (●), after 96h at 25°C. A : Macroscopic images are obtained using an automated robot that integrates incubation and high-resolution imaging. Microscopic observations of microcolonies, endpoint stained with the cell-permeant nucleic acid dye Syto 61, are obtained with confocal laser scanning microscopy. Representative pictures correspond to the microcolonies <t>isosurface</t> after fluorescence segmentation using the Imaris isosurface functions. B : Quantitative parameters extracted from the isosurface of microcolonies in Imaris software. Each point represents an independent replicate (n=9), bars correspond to the mean of volume of microcolonies log 10 (µm 3 ) and sphericity (A.U.). A two-way ANOVA was performed to assess the effect of food stress. When a significant effect of the environment was detected, the Bonferroni multiple comparisons test was applied to evaluate the level of significance. Asterisks indicate significant differences between environments (p<0.05).
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Microcolonies architecture in GSFM (▴) or S-GSFM (●), after 96h at 25°C. A : Macroscopic images are obtained using an automated robot that integrates incubation and high-resolution imaging. Microscopic observations of microcolonies, endpoint stained with the cell-permeant nucleic acid dye Syto 61, are obtained with confocal laser scanning microscopy. Representative pictures correspond to the microcolonies <t>isosurface</t> after fluorescence segmentation using the Imaris isosurface functions. B : Quantitative parameters extracted from the isosurface of microcolonies in Imaris software. Each point represents an independent replicate (n=9), bars correspond to the mean of volume of microcolonies log 10 (µm 3 ) and sphericity (A.U.). A two-way ANOVA was performed to assess the effect of food stress. When a significant effect of the environment was detected, the Bonferroni multiple comparisons test was applied to evaluate the level of significance. Asterisks indicate significant differences between environments (p<0.05).
Matlab Functions Isosurface, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isosurface+function+in/pm40373222-240-10-8?v=MathWorks+Inc
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MathWorks Inc isosurface function
Microcolonies architecture in GSFM (▴) or S-GSFM (●), after 96h at 25°C. A : Macroscopic images are obtained using an automated robot that integrates incubation and high-resolution imaging. Microscopic observations of microcolonies, endpoint stained with the cell-permeant nucleic acid dye Syto 61, are obtained with confocal laser scanning microscopy. Representative pictures correspond to the microcolonies <t>isosurface</t> after fluorescence segmentation using the Imaris isosurface functions. B : Quantitative parameters extracted from the isosurface of microcolonies in Imaris software. Each point represents an independent replicate (n=9), bars correspond to the mean of volume of microcolonies log 10 (µm 3 ) and sphericity (A.U.). A two-way ANOVA was performed to assess the effect of food stress. When a significant effect of the environment was detected, the Bonferroni multiple comparisons test was applied to evaluate the level of significance. Asterisks indicate significant differences between environments (p<0.05).
Isosurface Function, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isosurface+function+in/pm39996730-77-26-34?v=MathWorks+Inc
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MathWorks Inc matlab isosurface function
Microcolonies architecture in GSFM (▴) or S-GSFM (●), after 96h at 25°C. A : Macroscopic images are obtained using an automated robot that integrates incubation and high-resolution imaging. Microscopic observations of microcolonies, endpoint stained with the cell-permeant nucleic acid dye Syto 61, are obtained with confocal laser scanning microscopy. Representative pictures correspond to the microcolonies <t>isosurface</t> after fluorescence segmentation using the Imaris isosurface functions. B : Quantitative parameters extracted from the isosurface of microcolonies in Imaris software. Each point represents an independent replicate (n=9), bars correspond to the mean of volume of microcolonies log 10 (µm 3 ) and sphericity (A.U.). A two-way ANOVA was performed to assess the effect of food stress. When a significant effect of the environment was detected, the Bonferroni multiple comparisons test was applied to evaluate the level of significance. Asterisks indicate significant differences between environments (p<0.05).
Matlab Isosurface Function, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Microcolonies architecture in GSFM (▴) or S-GSFM (●), after 96h at 25°C. A : Macroscopic images are obtained using an automated robot that integrates incubation and high-resolution imaging. Microscopic observations of microcolonies, endpoint stained with the cell-permeant nucleic acid dye Syto 61, are obtained with confocal laser scanning microscopy. Representative pictures correspond to the microcolonies isosurface after fluorescence segmentation using the Imaris isosurface functions. B : Quantitative parameters extracted from the isosurface of microcolonies in Imaris software. Each point represents an independent replicate (n=9), bars correspond to the mean of volume of microcolonies log 10 (µm 3 ) and sphericity (A.U.). A two-way ANOVA was performed to assess the effect of food stress. When a significant effect of the environment was detected, the Bonferroni multiple comparisons test was applied to evaluate the level of significance. Asterisks indicate significant differences between environments (p<0.05).

Journal: bioRxiv

Article Title: Spatial growth in food-like matrices differentially modulates food-related stress responses but enhances digestive tolerance in major foodborne pathogens

doi: 10.64898/2026.03.05.710002

Figure Lengend Snippet: Microcolonies architecture in GSFM (▴) or S-GSFM (●), after 96h at 25°C. A : Macroscopic images are obtained using an automated robot that integrates incubation and high-resolution imaging. Microscopic observations of microcolonies, endpoint stained with the cell-permeant nucleic acid dye Syto 61, are obtained with confocal laser scanning microscopy. Representative pictures correspond to the microcolonies isosurface after fluorescence segmentation using the Imaris isosurface functions. B : Quantitative parameters extracted from the isosurface of microcolonies in Imaris software. Each point represents an independent replicate (n=9), bars correspond to the mean of volume of microcolonies log 10 (µm 3 ) and sphericity (A.U.). A two-way ANOVA was performed to assess the effect of food stress. When a significant effect of the environment was detected, the Bonferroni multiple comparisons test was applied to evaluate the level of significance. Asterisks indicate significant differences between environments (p<0.05).

Article Snippet: Raw fluorescence of each image was then binarised using the automated Imaris isosurface function.

Techniques: Incubation, Imaging, Staining, Confocal Laser Scanning Microscopy, Fluorescence, Software